Origin IGS: aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatatatccctctaaatttaattaaacgctattcacttttatctttagatgaaataaaagtgcggctaatattctcagtatttttttattttagtcaagttttattcattttaattaactattagccgaagaaatatttttataataaagtcattatttttctgtcaaaccaaaaaatattcatataaaggaagtcct Mask Tandem Repeat Region ================================================ aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Find is-nt database================================================ Query_seq: AA00920:AA00921|AA00920:AA00921:DNA-3-methyladenine glycosylase I:conserved hypothetical protein:->->:633274..633471 198 aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00920:AA00921|AA00920:AA00921:DNA-3-methyladenine glycosylase I:conserved hypothetical protein:->->:633274..633471 198 aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00920:AA00921|AA00920:AA00921:DNA-3-methyladenine glycosylase I:conserved hypothetical protein:->->:633274..633471 198 aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: - Start: 77 End: 193 ............................................................................ Y N I L I F L V Q S F Y F F V S F I L R V K I E D L S L L S Y R K I L N L P M ..... aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00920:AA00921|AA00920:AA00921:DNA-3-methyladenine glycosylase I:conserved hypothetical protein:->->:633274..633471 198 aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttattataaaaatatttcttcggctaatagttaattaaaatgaataaaacttgactaaaataaaaaaatactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt Intra-Species Hit: Count: 1 Min: 1 Max: 198 Len: 198 Subject: aact_AA00920_AA00921|DNA-3-methyladenine glycosylase I:conserved hypothetical protein|POSITIVE:POSITIVE|[633274,633471]|198 HSP 1 e-value: 4.0E-38 bit: 159.0 Len: 80 Query Start:119 Query End:198 Subject Strand: POSITIVE Subject Start: 119 Subject End: 198 ......................................................................................................................tactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt ......................................................................................................................tactgagaatattagccgcacttttatttcatctaaagataaaagtgaatagcgtttaattaaatttagagggatatatt HSP 2 e-value: 4.0E-23 bit: 109.0 Len: 55 Query Start:1 Query End:55 Subject Strand: POSITIVE Subject Start: 1 Subject End: 55 aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttatt............................................................................................................................................... aggacttcctttatatgaatattttttggtttgacagaaaaataatgactttatt............................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGACUUCCUUUAUAUGAAUAUUUUUUGGUUUGACAGAAAAAUAAUGACUUUAUUAUAAAAAUAUUUCUUCGGCUAAUAGUUAAUUAAAAUGAAUAAAACUUGACUAAAAUAAAAAAAUACUGAGAAUAUUAGCCGCACUUUUAUUUCAUCUAAAGAUAAAAGUGAAUAGCGUUUAAUUAAAUUUAGAGGGAUAUAUU .....((((((((........(((((((......))))))).....(((.(((((...............((((((((((((((................)))))))......................)))))))(((((((((((.......)))))))))))))))).)))..........))))))))...... (-29.83) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUAUCCCUCUAAAUUUAAUUAAACGCUAUUCACUUUUAUCUUUAGAUGAAAUAAAAGUGCGGCUAAUAUUCUCAGUAUUUUUUUAUUUUAGUCAAGUUUUAUUCAUUUUAAUUAACUAUUAGCCGAAGAAAUAUUUUUAUAAUAAAGUCAUUAUUUUUCUGUCAAACCAAAAAAUAUUCAUAUAAAGGAAGUCCU ..................................(((((...(((((.((((.((((((((((((((((((.....((((......))))(((((.(((..........))).))))).)))))))))......)))))))))...........(((((((.((......))))))))).)))).))))))))))... (-20.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 321 160 114 166 128 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 321 160 114 97 59 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 281 156 113 145 104 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 280 145 113 218 186 aact:AA00084|5end_aminotransferase_60814..61044_POSITIVE 280 145 113 68 36 aact:AA00083|5end_hypothetical_protein_60664..60894_POSITIVE 269 150 111 131 92 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 267 156 114 148 104 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 263 175 133 132 83 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 258 176 133 194 152 aact:AA02340|5end_hypothetical_protein_1598887..1599117_POSITIVE 258 143 114 151 122 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 253 144 113 137 106 aact:AA02814|5end_hypothetical_protein_1980180..1980410_POSITIVE 253 150 101 134 87 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 248 143 110 163 130 aact:AA00239|5end_hypothetical_protein_171213..171443_POSITIVE 247 142 114 121 93 aact:AA01024|5end_thiamine_biosynthesis_lipoprotein_precursor_716437..716667_POSITIVE 246 147 108 148 108 aact:AA02235|5end_stringent_starvation_protein_B_1521446..1521676_POSITIVE 246 147 108 43 3 aact:AA02234|5end_hypothetical_protein_1521341..1521571_POSITIVE 244 144 114 221 191 aact:AA01607|5end_oligopeptide_transporter,_periplasmic-binding_protein_1085644..1085874_POSITIVE 244 145 101 115 72 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 243 166 121 140 100 aact:AA02159|5end_conserved_hypothetical_protein_1477333..1477563_POSITIVE 238 147 102 80 38 aact:AA00530|5end_hypothetical_protein_362373..362603_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 321 160 114 41 3 aact:AA02094|3end_glycogen_phosphorylase;_glycogen_synthase_1428778..1428928_POSITIVE 311 168 122 56 13 aact:AA02540|3end_5-methyltetrahydropteroyltriglutamate-homocystein_e_methyltransferase_1769499..1769649_POSITIVE 281 156 113 54 13 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 280 145 113 91 59 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 269 150 111 54 15 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 267 156 114 52 8 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 258 176 133 112 70 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 258 143 114 146 117 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 257 174 133 142 93 aact:AA02025|3end_hypothetical_protein_1378851..1379001_POSITIVE 254 154 116 105 62 aact:AA02020|3end_conserved_hypothetical_protein_(possible_tetrapyrrole_methylase_family_protein)_1374742..1374892_POSITIVE 253 150 101 111 64 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 248 143 110 39 6 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 247 142 114 39 11 aact:AA01023|3end_conserved_hypothetical_protein_716435..716585_POSITIVE 246 147 108 144 104 aact:AA02234|3end_hypothetical_protein_1521522..1521672_POSITIVE 244 144 114 151 121 aact:AA01605|3end_hypothetical_protein_1085654..1085804_POSITIVE 244 145 101 134 91 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 243 166 121 44 4 aact:AA02158|3end_TonB-dependent_outer_membrane_receptor_1477317..1477467_POSITIVE 237 149 114 132 96 aact:AA01937|3end_sugar_fermentation_stimulation_protein_1309334..1309484_POSITIVE 237 155 114 100 60 aact:AA00579|3end_probable_DNA_recombination_protein_396955..397105_POSITIVE 235 146 114 126 94 aact:AA01044|3end_A/G-specific_adenine_glycosylase_727978..728128_POSITIVE