Origin IGS: atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaacacaccttgtctgaaactttgcgggcgctgcccgggaaataaaaatggcgttaggataacccgaaatggcattttatgctattattttccttaattttttaaggaggaaat Mask Tandem Repeat Region ================================================ atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Find is-nt database================================================ Query_seq: AA00914:AA00915|AA00914:AA00915:aminoacyl-histidine dipeptidase; Beta-alanyl-histidine dipeptidase:xanthine-guanine phosphoribosyltransferase:->->:629636..629749 114 atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00914:AA00915|AA00914:AA00915:aminoacyl-histidine dipeptidase; Beta-alanyl-histidine dipeptidase:xanthine-guanine phosphoribosyltransferase:->->:629636..629749 114 atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00914:AA00915|AA00914:AA00915:aminoacyl-histidine dipeptidase; Beta-alanyl-histidine dipeptidase:xanthine-guanine phosphoribosyltransferase:->->:629636..629749 114 atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Predict ORF larger than 30AA ================================================ atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00914:AA00915|AA00914:AA00915:aminoacyl-histidine dipeptidase; Beta-alanyl-histidine dipeptidase:xanthine-guanine phosphoribosyltransferase:->->:629636..629749 114 atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: aact_AA00914_AA00915|aminoacyl-histidine dipeptidase; Beta-alanyl-histidine dipeptidase:xanthine-guanine phosphoribosyltransferase|POSITIVE:POSITIVE|[629636, 629749]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt atttcctccttaaaaaattaaggaaaataatagcataaaatgccatttcgggttatcctaacgccatttttatttcccgggcagcgcccgcaaagtttcagacaaggtgtgttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUCCUCCUUAAAAAAUUAAGGAAAAUAAUAGCAUAAAAUGCCAUUUCGGGUUAUCCUAACGCCAUUUUUAUUUCCCGGGCAGCGCCCGCAAAGUUUCAGACAAGGUGUGUUU ......(((((((....)))))))........((((...))))......(((....))).(((((............(((((...)))))....(((...)))..))))).... (-22.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAACACACCUUGUCUGAAACUUUGCGGGCGCUGCCCGGGAAAUAAAAAUGGCGUUAGGAUAACCCGAAAUGGCAUUUUAUGCUAUUAUUUUCCUUAAUUUUUUAAGGAGGAAAU .......(((((((......(((.(((((...))))).)))........))))..))).........((((((((...)))))))).((((((((((....))))))))))... (-29.74) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 365 87 41 174 125 aact:AA00202|5end_conserved_hypothetical_protein_(possible_membrane_protein)_140546..140776_POSITIVE 360 109 62 149 86 aact:AA00287|5end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200158..200388_POSITIVE 341 86 41 58 15 aact:AA01857|5end_glutamate-ammonia-ligase_adenylyltransferase_1247347..1247577_POSITIVE 338 90 41 61 17 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 336 90 42 180 128 aact:AA01120|5end_conserved_hypothetical_protein_769449..769679_POSITIVE 335 90 42 47 15 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 329 106 62 130 86 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 328 98 53 71 15 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 320 90 41 159 96 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 316 88 41 230 184 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 316 92 51 37 4 aact:AA00069|5end_conserved_hypothetical_protein_52465..52695_POSITIVE 315 90 41 200 133 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 313 90 44 223 156 aact:AA01623|5end_alanine_racemase_1_1097077..1097307_POSITIVE 308 87 42 230 184 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 306 108 62 198 150 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 306 108 62 78 30 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 305 100 55 196 145 aact:AA02947|5end_riboflavin_biosynthesis_protein_2060871..2061101_POSITIVE 305 90 44 77 34 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 304 109 61 55 9 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 302 110 63 63 16 aact:AA00115|5end_glutamate--cysteine_ligase_(gamma-glutamylcysteine_synthetase)_(gamma-ECS)_84027..84257_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 364 89 42 145 93 aact:AA02352|3end_glycine_betaine/carnitine/choline_ABC_transporter_1611114..1611264_POSITIVE 338 90 41 54 10 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 335 90 42 63 31 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 334 89 45 144 84 aact:AA00286|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200260..200410_POSITIVE 329 106 62 127 83 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 320 90 41 64 1 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 316 88 41 135 89 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 305 100 55 115 64 aact:AA02946|3end_virulence_factor_protein_2060870..2061020_POSITIVE 305 90 44 66 23 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 302 88 44 81 22 aact:AA02268|3end_hypothetical_protein_1545973..1546123_POSITIVE 294 90 42 130 64 aact:AA01656|3end_maltose/maltodextrin_transport_ATP-binding_protein_1122735..1122885_POSITIVE 293 92 62 40 13 aact:AA01740|3end_conserved_hypothetical_protein_1177243..1177393_POSITIVE 290 109 62 69 27 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 288 88 41 65 2 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 285 95 52 127 84 aact:AA02144|3end_iron_(III)_ABC_transporter,_permease_protein_1468679..1468829_POSITIVE 284 93 62 141 94 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 283 90 41 111 58 aact:AA01679|3end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1135236..1135386_POSITIVE 283 108 78 52 11 aact:AA00600|3end_ribosome_recycling_factor_(ribosome_releasing_factor)_408939..409089_POSITIVE 282 95 51 59 10 aact:AA01043|3end_conserved_hypothetical_protein_726810..726960_POSITIVE 281 90 43 146 75 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE