Origin IGS: gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagaaaaattcggaatgtgtaaaactatttcggaaaatcagggtcaaacgggcattcttgcttcagtcgccatttatattaaagccacatatagttacacgtaattcagacttttaaggttgctgtagcgaggcaatgattcatctattttcaacctgaaggaatatc Mask Tandem Repeat Region ================================================ gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Find is-nt database================================================ Query_seq: AA00864:AA00865|AA00864:AA00865:conserved hypothetical protein:conserved hypothetical protein:->->:599444..599612 169 gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00864:AA00865|AA00864:AA00865:conserved hypothetical protein:conserved hypothetical protein:->->:599444..599612 169 gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00864:AA00865|AA00864:AA00865:conserved hypothetical protein:conserved hypothetical protein:->->:599444..599612 169 gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Predict ORF larger than 30AA ================================================ gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00864:AA00865|AA00864:AA00865:conserved hypothetical protein:conserved hypothetical protein:->->:599444..599612 169 gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Intra-Species Hit: Count: 1 Min: 1 Max: 169 Len: 169 Subject: aact_AA00864_AA00865|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[599444,599612]|169 HSP 1 e-value: 3.0E-91 bit: 335.0 Len: 169 Query Start:1 Query End:169 Subject Strand: POSITIVE Subject Start: 1 Subject End: 169 gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta gatattccttcaggttgaaaatagatgaatcattgcctcgctacagcaaccttaaaagtctgaattacgtgtaactatatgtggctttaatataaatggcgactgaagcaagaatgcccgtttgaccctgattttccgaaatagttttacacattccgaatttttctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUAUUCCUUCAGGUUGAAAAUAGAUGAAUCAUUGCCUCGCUACAGCAACCUUAAAAGUCUGAAUUACGUGUAACUAUAUGUGGCUUUAAUAUAAAUGGCGACUGAAGCAAGAAUGCCCGUUUGACCCUGAUUUUCCGAAAUAGUUUUACACAUUCCGAAUUUUUCUUA .........((((((..((..........((((((((..((....)).......((((((......((((((....)))))))))))).........))))).))).(((....)))...))..).)))))......((((.(((((..........)))))))))... (-25.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAAAAUUCGGAAUGUGUAAAACUAUUUCGGAAAAUCAGGGUCAAACGGGCAUUCUUGCUUCAGUCGCCAUUUAUAUUAAAGCCACAUAUAGUUACACGUAAUUCAGACUUUUAAGGUUGCUGUAGCGAGGCAAUGAUUCAUCUAUUUUCAACCUGAAGGAAUAUC .........((((((((((.....)).))))))))...(((((...(((..((...(((((((((.((.((.............)).)).....((((((.((((((...........))))))))))))))))))).))....))..)))....)))))......... (-32.72) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 140 93 150 88 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 313 130 82 125 81 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 302 158 114 151 105 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 297 158 115 34 1 aact:AA00914|5end_aminoacyl-histidine_dipeptidase;_Beta-alanyl-histidine_dipeptidase_628038..628268_POSITIVE 291 139 96 75 21 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 288 128 81 53 3 aact:AA00309|5end_3-hydroxyisobutyrate_dehydrogenase;_2-hydroxy-3-oxopropionate_reductase_209285..209515_POSITIVE 286 127 81 146 86 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 283 75 32 161 110 aact:AA01347|5end_conserved_hypothetical_protein_913209..913439_POSITIVE 279 138 96 183 124 aact:AA01629|5end_phosphatidylglycerophosphatase_A_1101881..1102111_POSITIVE 277 138 96 185 136 aact:AA02579|5end_aminotransferase_nifS_protein_1795773..1796003_POSITIVE 277 130 81 223 165 aact:AA00950|5end_conserved_hypothetical_protein_656922..657152_POSITIVE 277 138 94 142 98 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 272 159 111 140 81 aact:AA01194|5end_acyl_carrier_protein_819595..819825_POSITIVE 268 140 96 79 23 aact:AA03014|5end_conserved_hypothetical_protein_2104815..2105045_POSITIVE 267 128 83 116 65 aact:AA02523|5end_conserved_hypothetical_protein_1752990..1753220_POSITIVE 267 158 116 183 128 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 265 62 24 43 5 aact:AA01872|5end_conserved_hypothetical_protein_1258804..1259034_POSITIVE 258 137 97 179 138 aact:AA02538|5end_conserved_hypothetical_protein_1765187..1765417_POSITIVE 258 138 96 209 173 aact:AA02407|5end_thymidine_kinase_1652666..1652896_POSITIVE 258 139 92 66 17 aact:AA01366|5end_16S_rRNA_processing_protein_923117..923347_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 140 93 150 88 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 302 158 114 137 91 aact:AA02937|3end_conserved_hypothetical_protein_2056947..2057097_POSITIVE 301 123 82 148 109 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 283 75 32 133 82 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE 277 138 94 139 95 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 272 159 111 137 78 aact:AA01193|3end_AMP-binding_enzyme_819672..819822_POSITIVE 267 128 83 76 25 aact:AA02521|3end_conserved_hypothetical_protein_1753030..1753180_POSITIVE 256 51 11 139 100 aact:AA02762|3end_ribonuclease_H_1933708..1933858_POSITIVE 252 124 81 120 67 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 251 124 96 114 91 aact:AA00468|3end_tryptophan-specific_transport_protein_/_tyrosine-specific_transport_protein_326124..326274_POSITIVE 249 128 81 60 12 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 248 126 84 125 85 aact:AA02691|3end_hypothetical_protein_1876315..1876465_POSITIVE 248 126 84 45 5 aact:AA02689|3end_hydrogenase-4_component_F_1876235..1876385_POSITIVE 248 130 81 147 102 aact:AA01274|3end_hypothetical_protein_864832..864982_POSITIVE 247 138 109 81 52 aact:AA01453|3end_D-xylose_isomerase_974853..975003_POSITIVE 246 139 95 79 30 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 245 138 92 145 92 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 245 140 97 50 7 aact:AA01519.1|3end_conserved_hypothetical_protein_1022920..1023070_POSITIVE 244 144 111 150 122 aact:AA01146|3end_conserved_hypothetical_protein_784770..784920_POSITIVE 243 51 22 83 56 aact:AA02169|3end_hypothetical_protein_1484664..1484814_POSITIVE