Origin IGS: aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagaggcaaactcacagaacagcggcactagcaaaagataagcggcagttacgccgctctatcttctctttattttacattgtaatttttatttaccattggtaatggatt Mask Tandem Repeat Region ================================================ aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Find is-nt database================================================ Query_seq: AA00847:AA00849|AA00847:AA00849:S-adenosyl-dependent methyltransferase:MraZ cell division protein:->->:586066..586177 112 aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00847:AA00849|AA00847:AA00849:S-adenosyl-dependent methyltransferase:MraZ cell division protein:->->:586066..586177 112 aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00847:AA00849|AA00847:AA00849:S-adenosyl-dependent methyltransferase:MraZ cell division protein:->->:586066..586177 112 aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Predict ORF larger than 30AA ================================================ aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 76 HP_score: -4.0 Tail_Score: -5.29846 Start: 27 End: 37 Full_Region: tatcttctctttatt ttac att gtaa tttttatttaccatt ..........................ttacattgtaa........................................................................... TransTerm Strand: - Conf: 65 HP_score: -3.1 Tail_Score: -4.73092 Start: 44 End: 80 Full_Region: gaacagcggcactag caaaagataagcggc agttac gccgctctatcttctc tttattttacattgt ...........................................caaaagataagcggcagttacgccgctctatcttctc................................ TransTerm Strand: + Conf: 83 HP_score: -8.1 Tail_Score: -4.30576 Start: 49 End: 75 Full_Region: GTAAAATAAAGAGAA GATAGAGCGGCG TAAC TGCCGCTTATC TTTTGCTAGTGCCGC ................................................GATAGAGCGGCGTAACTGCCGCTTATC..................................... TransTerm Strand: + Conf: 79 HP_score: -8.2 Tail_Score: -4.00084 Start: 55 End: 70 Full_Region: TAAAGAGAAGATAGA GCGGCG TAAC TGCCGC TTATCTTTTGCTAGT ......................................................GCGGCGTAACTGCCGC.......................................... Find igs database================================================ Query_seq: AA00847:AA00849|AA00847:AA00849:S-adenosyl-dependent methyltransferase:MraZ cell division protein:->->:586066..586177 112 aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: aact_AA00847_AA00849|S-adenosyl-dependent methyltransferase:MraZ cell division protein|POSITIVE:POSITIVE|[586066,586177]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta aatccattaccaatggtaaataaaaattacaatgtaaaataaagagaagatagagcggcgtaactgccgcttatcttttgctagtgccgctgttctgtgagtttgcctctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCCAUUACCAAUGGUAAAUAAAAAUUACAAUGUAAAAUAAAGAGAAGAUAGAGCGGCGUAACUGCCGCUUAUCUUUUGCUAGUGCCGCUGUUCUGUGAGUUUGCCUCUUA ..............(((((((.....................(((((((((.((((((((....))))))))))))))).)).....(((......))).)))))))..... (-27.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAGGCAAACUCACAGAACAGCGGCACUAGCAAAAGAUAAGCGGCAGUUACGCCGCUCUAUCUUCUCUUUAUUUUACAUUGUAAUUUUUAUUUACCAUUGGUAAUGGAUU .(((((................((.......))..((((((((((((......)))))).))))))))))).........................((((.....))))... (-23.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 396 108 66 191 138 aact:AA00351|5end_50S_ribosomal_protein_L7/L12_234155..234385_POSITIVE 396 108 66 187 134 aact:AA00353|5end_hypothetical_protein_234151..234381_POSITIVE 394 90 54 200 161 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 382 92 56 144 104 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 377 100 52 102 40 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 372 97 55 47 2 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 372 100 52 60 3 aact:AA01743|5end_lysyl-tRNA_synthetase_1177808..1178038_POSITIVE 371 99 51 179 120 aact:AA00579|5end_probable_DNA_recombination_protein_395265..395495_POSITIVE 364 90 46 210 163 aact:AA01682|5end_molybdate_ABC_transporter_protein_1135568..1135798_POSITIVE 353 107 64 180 134 aact:AA00805|5end_fumarate_reductase_iron-sulfur_protein_551583..551813_POSITIVE 350 95 53 62 14 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 349 99 56 191 136 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 347 90 41 179 114 aact:AA00741|5end_possible_2-oxoglutarate/malate_transporter_506659..506889_POSITIVE 341 97 57 115 75 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 337 106 62 219 164 aact:AA02325|5end_membrane_protein;_possible_ABC_transporter,_permease_1587309..1587539_POSITIVE 336 93 54 157 124 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 335 100 54 202 142 aact:AA01434|5end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_961631..961861_POSITIVE 334 99 51 214 148 aact:AA00770|5end_outer_membrane_protein_precursor_530788..531018_POSITIVE 332 89 41 88 29 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 330 99 51 155 92 aact:AA02827|5end_conserved_hypothetical_protein_1987021..1987251_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 396 108 66 145 92 aact:AA00350|3end_50S_ribosomal_protein_L10_234189..234339_POSITIVE 382 92 56 79 39 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 355 82 43 48 9 aact:AA00847|3end_S-adenosyl-dependent_methyltransferase_586005..586155_POSITIVE 351 99 55 150 100 aact:AA01393|3end_ABC_transporter,_ATP-binding_protein_942029..942179_POSITIVE 349 99 56 111 56 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 327 100 58 98 61 aact:AA02014|3end_probable_hemolysin_1370977..1371127_POSITIVE 323 106 63 65 18 aact:AA00132|3end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_95299..95449_POSITIVE 318 109 64 111 61 aact:AA01890|3end_cell-cycle_protein_1275967..1276117_POSITIVE 318 95 56 149 99 aact:AA00998|3end_small_protein_A_homolog_699015..699165_POSITIVE 316 100 56 76 38 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 308 99 52 150 112 aact:AA00943|3end_conserved_hypothetical_protein_654542..654692_POSITIVE 305 99 56 64 13 aact:AA01759|3end_hydrogenase_isoenzymes_formation_protein_1189864..1190014_POSITIVE 304 93 51 149 104 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 304 107 64 74 8 aact:AA02019|3end_hypothetical_protein_1373925..1374075_POSITIVE 304 97 57 44 1 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 302 95 63 80 42 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 302 106 64 147 99 aact:AA00804|3end_fumarate_reductase_flavoprotein_subunit_A_551655..551805_POSITIVE 302 97 55 41 3 aact:AA00374|3end_short-chain_fatty_acids_transporter_253101..253251_POSITIVE 301 92 53 48 4 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 299 90 41 128 77 aact:AA01365|3end_30S_ribosomal_protein_S16_923168..923318_POSITIVE