Origin IGS: aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataaaatgattctaagagctatatttcttcggaaatatagctctttttttcgctaccttttcataaagccaccttaaaatagtggatttttttgtctttcttttccataaaagtgcggttaaaaaagtaatatattttcaatacttattttatttattgcctttgaggtgtaacaaatgtgaatacaaaggaaatatt Mask Tandem Repeat Region ================================================ aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Find is-nt database================================================ Query_seq: AA00825:AA00826|AA00825:AA00826:P-protein (chorismate mutase/prephenate dehydratase):UDP-3-O-acyl-GlcNAc deacetylase:->->:568318..568517 200 aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00825:AA00826|AA00825:AA00826:P-protein (chorismate mutase/prephenate dehydratase):UDP-3-O-acyl-GlcNAc deacetylase:->->:568318..568517 200 aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00825:AA00826|AA00825:AA00826:P-protein (chorismate mutase/prephenate dehydratase):UDP-3-O-acyl-GlcNAc deacetylase:->->:568318..568517 200 aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 68 End: 184 ................................................................... M T F L T A L L W K R K T K K S T I L R W L Y E K V A K K R A I F P K K Y S S ................ Protein_Len: 40 Strand: - Start: 1 End: 120 I N G K Y E C K N C R L P L L Y F L Y Q F Y I V K K V A S K H F L F V F F D V M ................................................................................ aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 85 HP_score: -11.2 Tail_Score: -3.09131 Start: 158 End: 181 Full_Region: GTAGCGAAAAAAAGA GCTATATTTC CGAA GAAATATAGC TCTTAGAATCATTTT .............................................................................................................................................................GCTATATTTCCGAAGAAATATAGC................... Find igs database================================================ Query_seq: AA00825:AA00826|AA00825:AA00826:P-protein (chorismate mutase/prephenate dehydratase):UDP-3-O-acyl-GlcNAc deacetylase:->->:568318..568517 200 aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Intra-Species Hit: Count: 1 Min: 1 Max: 200 Len: 200 Subject: aact_AA00825_AA00826|P-protein (chorismate mutase/prephenate dehydratase):UDP-3-O-acyl-GlcNAc deacetylase|POSITIVE:POSITIVE|[568318,568517]|200 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 200 Query Start:1 Query End:200 Subject Strand: POSITIVE Subject Start: 1 Subject End: 200 aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacnnnnnnntccactattttaaggtggctttatgaaaaggtagcgnnnnnnngagctatatttccgaagaaatatagctcttagaatcattttatta aatatttcctttgtattcacatttgttacacctcaaaggcaataaataaaataagtattgaaaatatattacttttttaaccgcacttttatggaaaagaaagacaaaaaaatccactattttaaggtggctttatgaaaaggtagcgaaaaaaagagctatatttccgaagaaatatagctcttagaatcattttatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUUUCCUUUGUAUUCACAUUUGUUACACCUCAAAGGCAAUAAAUAAAAUAAGUAUUGAAAAUAUAUUACUUUUUUAACCGCACUUUUAUGGAAAAGAAAGACAAAAAAAUCCACUAUUUUAAGGUGGCUUUAUGAAAAGGUAGCGAAAAAAAGAGCUAUAUUUCCGAAGAAAUAUAGCUCUUAGAAUCAUUUUAUUA ..((((.((((((.....((....)).......)))))).)))).....((((((...(((.......(((((((((((......(((((.(....).)))))......(((.(((((.......))))).))).))))))))))).......((((((((((((((....))))))))))))))....))).)))))). (-43.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAAAUGAUUCUAAGAGCUAUAUUUCUUCGGAAAUAUAGCUCUUUUUUUCGCUACCUUUUCAUAAAGCCACCUUAAAAUAGUGGAUUUUUUUGUCUUUCUUUUCCAUAAAAGUGCGGUUAAAAAAGUAAUAUAUUUUCAAUACUUAUUUUAUUUAUUGCCUUUGAGGUGUAACAAAUGUGAAUACAAAGGAAAUAUU .........((....((((((((((((((....))))))))))))))....))....((((((((((....(((((((((...(((((.................)))))......(((((.((.((((((((((.......))).))))))).)).))))).))))))))).......)))))....)))))....... (-47.73) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 130 81 210 142 aact:AA02080|5end_ABC_transporter,_ATP-binding/permease_1412744..1412974_POSITIVE 301 130 81 78 10 aact:AA02078|5end_hypothetical_protein_1412612..1412842_POSITIVE 281 120 77 183 127 aact:AA01694|5end_PTS_system,_IIA_component_1149929..1150159_POSITIVE 279 94 51 139 97 aact:AA02512|5end_O-succinylbenzoate--CoA_ligase_1745379..1745609_POSITIVE 271 96 59 103 63 aact:AA00233|5end_hypothetical_protein_162339..162569_POSITIVE 266 168 126 73 34 aact:AA02811|5end_hypothetical_protein_1979582..1979812_POSITIVE 256 184 146 78 47 aact:AA02151|5end_ferric_enterobactin_receptor;_TonB-dependent_outer_membrane_siderophore_receptor_protein_1470856..1471086_POSITIVE 253 90 44 95 47 aact:AA00235|5end_hypothetical_protein_168596..168826_POSITIVE 252 119 77 209 170 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 248 129 81 136 84 aact:AA02024|5end_acyl_carrier_protein_phosphodiesterase_1378144..1378374_POSITIVE 246 104 73 126 96 aact:AA02785|5end_ribose_ABC_transporter,_permease_1951808..1952038_POSITIVE 244 193 151 122 84 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 243 116 75 173 127 aact:AA01371|5end_rod_shape-determining_protein_926579..926809_POSITIVE 242 169 127 128 86 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 241 120 73 219 172 aact:AA01965|5end_poly(A)_polymerase_1329478..1329708_POSITIVE 240 95 59 84 48 aact:AA02213|5end_hypothetical_protein_1506996..1507226_POSITIVE 237 91 58 154 121 aact:AA01069|5end_translation_elongation_factor_(EF-Ts)_739775..740005_POSITIVE 237 91 58 48 15 aact:AA01068|5end_hypothetical_protein_739669..739899_POSITIVE 234 102 61 178 135 aact:AA02128|5end_DNA_topoisomerase_I_1451395..1451625_POSITIVE 234 129 81 157 103 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 130 81 94 26 aact:AA02077|3end_probable_outer_membrane_protein_1412708..1412858_POSITIVE 289 107 74 140 104 aact:AA02078|3end_hypothetical_protein_1412793..1412943_POSITIVE 281 120 77 103 47 aact:AA01692|3end_hexulose-6-phosphate_synthase_1149929..1150079_POSITIVE 279 94 51 127 85 aact:AA02511|3end_transmembrane_protein_1745447..1745597_POSITIVE 271 96 59 83 43 aact:AA00231|3end_long-chain-fatty-acid--CoA_ligase_162399..162549_POSITIVE 266 172 135 77 34 aact:AA01508|3end_anaerobic_C4-dicarboxylate_transporter_1014597..1014747_POSITIVE 256 184 146 127 96 aact:AA02150|3end_conserved_hypothetical_protein_1470985..1471135_POSITIVE 253 90 44 60 12 aact:AA00234|3end_collagen_adhesin_168641..168791_POSITIVE 246 104 73 37 7 aact:AA02784|3end_NAD+_dependent_acetaldehyde-alcohol_dehydrogenase,_iron-containing_1951799..1951949_POSITIVE 245 95 54 81 41 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 244 193 151 75 37 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 243 116 75 100 54 aact:AA01370|3end_rod_shape-determining_protein_926586..926736_POSITIVE 242 169 127 74 32 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 241 120 73 99 52 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 240 95 59 86 50 aact:AA02212|3end_ribonucleoside-diphosphate_reductase_beta_chain_1507078..1507228_POSITIVE 237 91 58 149 116 aact:AA01068|3end_hypothetical_protein_739850..740000_POSITIVE 234 102 61 87 44 aact:AA02126|3end_conserved_hypothetical_protein_1451384..1451534_POSITIVE 233 109 69 72 25 aact:AA02937|3end_conserved_hypothetical_protein_2056947..2057097_POSITIVE 233 187 142 129 90 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 232 97 55 73 30 aact:AA00135|3end_S-adenosylmethionine:_2-demethylmenaquinone_methyltransferase_(menaquinone_biosynthesis_protein)_96880..97030_POSITIVE