Origin IGS: acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaactttccttaaacttgattatgttttaagctaattatctttgaatttccatttcacgtccaccacattaatttctaactgttctacaacacccttatccagccaatgttaataccccacgcatgggaaccgtatgttgagggtgaatgataggttcttgaccgcattttattaacacgcgt Mask Tandem Repeat Region ================================================ acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Find is-nt database================================================ Query_seq: AA00813:AA00814|AA00813:AA00814:hypothetical protein:conserved hypothetical protein:->->:559082..559264 183 acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00813:AA00814|AA00813:AA00814:hypothetical protein:conserved hypothetical protein:->->:559082..559264 183 acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00813:AA00814|AA00813:AA00814:hypothetical protein:conserved hypothetical protein:->->:559082..559264 183 acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Predict ORF larger than 30AA ================================================ acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00813:AA00814|AA00813:AA00814:hypothetical protein:conserved hypothetical protein:->->:559082..559264 183 acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Intra-Species Hit: Count: 1 Min: 1 Max: 183 Len: 183 Subject: aact_AA00813_AA00814|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[559082,559264]|183 HSP 1 e-value: 1.0E-99 bit: 363.0 Len: 183 Query Start:1 Query End:183 Subject Strand: POSITIVE Subject Start: 1 Subject End: 183 acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt acgcgtgttaataaaatgcggtcaagaacctatcattcaccctcaacatacggttcccatgcgtggggtattaacattggctggataagggtgttgtagaacagttagaaattaatgtggtggacgtgaaatggaaattcaaagataattagcttaaaacataatcaagtttaaggaaagttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGCGUGUUAAUAAAAUGCGGUCAAGAACCUAUCAUUCACCCUCAACAUACGGUUCCCAUGCGUGGGGUAUUAACAUUGGCUGGAUAAGGGUGUUGUAGAACAGUUAGAAAUUAAUGUGGUGGACGUGAAAUGGAAAUUCAAAGAUAAUUAGCUUAAAACAUAAUCAAGUUUAAGGAAAGUUU .(((((.........)))))........((..(((((((((....((((.....(((((....)))))........(((((((.((((.....))))....)))))))......)))))))))..))))...))............((((..(((.((((........)))).)))..)))). (-30.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAACUUUCCUUAAACUUGAUUAUGUUUUAAGCUAAUUAUCUUUGAAUUUCCAUUUCACGUCCACCACAUUAAUUUCUAACUGUUCUACAACACCCUUAUCCAGCCAAUGUUAAUACCCCACGCAUGGGAACCGUAUGUUGAGGGUGAAUGAUAGGUUCUUGACCGCAUUUUAUUAACACGCGU .........(((((..((((((.((.....))))))))..)))))...........((((..............................((((((((...(((....))).....((((....))))..........)))))))).(((...((((...)))).)))...........)))) (-25.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 127 82 150 100 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 336 99 52 186 131 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 323 97 51 65 19 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 322 100 51 81 28 aact:AA02358|5end_homoserine_kinase_1617128..1617358_POSITIVE 321 90 41 55 1 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 316 59 17 209 157 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 314 110 61 55 6 aact:AA00591|5end_ABC_transporter,_ATP-binding_protein_403613..403843_POSITIVE 314 110 61 69 16 aact:AA00426|5end_conserved_hypothetical_protein_288027..288257_POSITIVE 313 70 38 197 162 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 309 109 61 80 38 aact:AA02554|5end_conserved_hypothetical_protein_1779735..1779965_POSITIVE 308 74 37 116 78 aact:AA01128|5end_hypothetical_protein_774030..774260_POSITIVE 303 100 52 75 19 aact:AA02789|5end_oxidoreductase-dehydrogenase_1956641..1956871_POSITIVE 303 97 52 196 158 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 295 89 41 84 39 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 294 99 51 122 75 aact:AA01108|5end_hypothetical_protein_763646..763876_POSITIVE 293 93 51 56 16 aact:AA02609|5end_ABC_transporter,_ATP-binding_protein/permease_1818446..1818676_POSITIVE 292 25 1 136 108 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 291 96 56 73 17 aact:AA01116|5end_hypothetical_protein_767629..767859_POSITIVE 291 122 81 199 149 aact:AA00141|5end_hypoxanthine_phosphoribosyl_transferase_(HPRT)_100814..101044_POSITIVE 288 97 51 60 10 aact:AA01099|5end_dimethyladenosine_transferase_758542..758772_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 127 82 142 92 aact:AA00576|3end_conserved_hypothetical_protein_(possible_translation_factor)_394648..394798_POSITIVE 322 100 51 65 12 aact:AA02357|3end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1617192..1617342_POSITIVE 321 90 41 83 29 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 320 96 51 68 9 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 294 99 51 150 103 aact:AA01107|3end_adenylosuccinate_lyase_763754..763904_POSITIVE 292 25 1 146 118 aact:AA00719|3end_hypothetical_protein_494829..494979_POSITIVE 292 25 1 40 12 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 283 93 51 105 62 aact:AA02666|3end_DNA_helicase_II_1856583..1856733_POSITIVE 277 93 52 142 109 aact:AA02459|3end_conserved_hypothetical_protein_1700643..1700793_POSITIVE 275 86 47 145 97 aact:AA03008|3end_GTP-binding_protein_era_homolog_(GTPase)_2101072..2101222_POSITIVE 273 97 52 92 48 aact:AA00254|3end_penicillin-binding_protein_5_178774..178924_POSITIVE 272 100 53 105 62 aact:AA02601|3end_ATP-dependent_helicase_1813309..1813459_POSITIVE 272 91 51 95 58 aact:AA01523|3end_conserved_hypothetical_protein_1023569..1023719_POSITIVE 271 96 53 151 102 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 270 91 51 66 20 aact:AA00092|3end_serine_transporter_69716..69866_POSITIVE 269 96 57 85 42 aact:AA02631|3end_undecaprenyl-phosphate_galactosephosphotransferase_homolog_1831964..1832114_POSITIVE 264 97 59 67 30 aact:AA02677|3end_formate_dehydrogenase,_beta_subunit_1865105..1865255_POSITIVE 263 90 41 82 29 aact:AA02700|3end_succinyl-CoA_synthase,_alpha_subunit_1884205..1884355_POSITIVE 263 94 51 138 102 aact:AA02678|3end_formate_dehydrogenase,_gamma_subunit_1865523..1865673_POSITIVE 263 85 50 147 112 aact:AA00424|3end_hypothetical_protein_287403..287553_POSITIVE