Origin IGS: attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacatttaaaaattagttcaattcaatatgtcatgtgaaattaaacttaaaaattcacagtttcccttgtttaaattagtttaatttaatatgctgtattgagttaaactaattttgagagtgaat Mask Tandem Repeat Region ================================================ attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Find is-nt database================================================ Query_seq: AA00811:AA00812|AA00811:AA00812:conserved hypothetical protein:ATPase (PP-loop superfamily), confers aluminum resistance:->->:558088..558214 127 attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00811:AA00812|AA00811:AA00812:conserved hypothetical protein:ATPase (PP-loop superfamily), confers aluminum resistance:->->:558088..558214 127 attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00811:AA00812|AA00811:AA00812:conserved hypothetical protein:ATPase (PP-loop superfamily), confers aluminum resistance:->->:558088..558214 127 attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Predict ORF larger than 30AA ================================================ attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00811:AA00812|AA00811:AA00812:conserved hypothetical protein:ATPase (PP-loop superfamily), confers aluminum resistance:->->:558088..558214 127 attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: aact_AA00811_AA00812|conserved hypothetical protein:ATPase (PP-loop superfamily), confers aluminum resistance|POSITIVE:POSITIVE|[558088,558214]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta attcactctcaaaattagtttaactcaatacagcatattaaattaaactaatttaaacaagggaaactgtgaatttttaagtttaatttcacatgacatattgaattgaactaatttttaaatgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCACUCUCAAAAUUAGUUUAACUCAAUACAGCAUAUUAAAUUAAACUAAUUUAAACAAGGGAAACUGUGAAUUUUUAAGUUUAAUUUCACAUGACAUAUUGAAUUGAACUAAUUUUUAAAUGUUA ..........(((((((((((((.((((((...(((.(.((((((((((((((((.....((....)).))))))....)))))))))).).)))...)))))).)))))))))))))......... (-29.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAUUUAAAAAUUAGUUCAAUUCAAUAUGUCAUGUGAAAUUAAACUUAAAAAUUCACAGUUUCCCUUGUUUAAAUUAGUUUAAUUUAAUAUGCUGUAUUGAGUUAAACUAAUUUUGAGAGUGAAU ...(((((.((((((((((.(((((((((((.(((((.((((((((((.........((((......)))).......)))))))))).))))).))))))))))).))))))))))..)))))... (-34.89) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 173 39 6 222 193 aact:AA01104|5end_conserved_hypothetical_protein_761155..761385_POSITIVE 172 110 61 216 173 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 169 72 32 110 65 aact:AA02265|5end_exodeoxyribonuclease_V,_alpha_chain_1539701..1539931_POSITIVE 167 93 59 148 117 aact:AA01668|5end_transcription_elongation_factor_1129830..1130060_POSITIVE 164 78 31 165 118 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 159 110 61 87 37 aact:AA00044|5end_formate_dependent_nitrite_reductase,_Fe-S_protein/_anaerobic_DMSO_reductase_B_35340..35570_POSITIVE 154 90 58 185 155 aact:AA00782|5end_conserved_hypothetical_protein_538723..538953_POSITIVE 145 111 96 223 208 aact:AA02574|5end_dGTP_triphosphohydrolase_1792395..1792625_POSITIVE 145 82 61 139 118 aact:AA00553|5end_NapD_component_of_periplasmic_nitrate_reductase_379851..380081_POSITIVE 144 63 30 115 84 aact:AA01498|5end_conserved_hypothetical_protein_1007957..1008187_POSITIVE 143 74 31 114 75 aact:AA02830|5end_colicin_tolerance_protein_1988729..1988959_POSITIVE 143 64 28 49 17 aact:AA02135|5end_hypothetical_protein_1458886..1459116_POSITIVE 143 109 61 117 69 aact:AA02092|5end_glycogen_synthase_1424674..1424904_POSITIVE 142 99 61 111 66 aact:AA02105|5end_killing_protein_1431885..1432115_POSITIVE 142 64 26 108 74 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 140 105 61 64 19 aact:AA01043|5end_conserved_hypothetical_protein_726455..726685_POSITIVE 139 77 31 136 76 aact:AA02399|5end_30S_ribosomal_protein_S21_1650900..1651130_POSITIVE 138 81 42 159 130 aact:AA02173|5end_anthranilate_synthase_component_I_1486741..1486971_POSITIVE 138 71 46 145 118 aact:AA01476|5end_hypothetical_protein_991402..991632_POSITIVE 137 90 59 73 44 aact:AA02005|5end_vancomycin_sensitivity;_SanA_protein_1363143..1363373_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 177 118 80 80 42 aact:AA00811|3end_conserved_hypothetical_protein_558027..558177_POSITIVE 173 39 6 80 51 aact:AA01102|3end_pyrimidine_operon_regulatory_protein_761093..761243_POSITIVE 169 72 32 94 49 aact:AA02263|3end_conserved_hypothetical_protein_1539765..1539915_POSITIVE 164 78 31 134 87 aact:AA01546|3end_deoxyribose_operon_repressor_1036320..1036470_POSITIVE 159 110 61 87 37 aact:AA00043|3end_formate_dependent_nitrite_reductase,_transmembrane_protein_35420..35570_POSITIVE 145 82 61 102 81 aact:AA00552|3end_periplasmic_nitrate_reductase_precursor_379894..380044_POSITIVE 144 63 30 55 24 aact:AA01497|3end_MioC_protein_1007977..1008127_POSITIVE 143 74 31 96 57 aact:AA02829|3end_peptidoglycan-associated_outer_membrane_1988791..1988941_POSITIVE 143 64 28 150 118 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 142 64 26 46 12 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 140 105 61 58 13 aact:AA01042|3end_membrane-bound_lytic_murein_transglycosylase_C_726529..726679_POSITIVE 139 91 57 139 111 aact:AA01250|3end_nadAB_transcriptional_regulator_851780..851930_POSITIVE 138 98 60 115 73 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 138 71 46 93 66 aact:AA01475|3end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_991430..991580_POSITIVE 136 109 65 59 22 aact:AA00186|3end_o-succinylbenzoate-CoA_synthase;_OSB_synthase_132445..132595_POSITIVE 134 106 69 124 82 aact:AA01528|3end_probable_oxidoreductase_1027260..1027410_POSITIVE 134 103 61 133 81 aact:AA00151|3end_polynucleotide_phosphorylase_111071..111221_POSITIVE 132 104 66 41 1 aact:AA01954|3end_excinuclease_ABC,_subunit_C_1322314..1322464_POSITIVE 131 97 60 121 82 aact:AA00279|3end_8-amino-7-oxonoanoate_synthase_196514..196664_POSITIVE 130 93 58 114 77 aact:AA01211|3end_ATP-dependent_Clp_protease,_ATPase_subunit_829682..829832_POSITIVE