Origin IGS: accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agcttttcctcgtaaaagtgagtaagttttttaatgattaagtttattttaaggataaaaagttgatattttcaataggtttaaaattaatgtaaaaattccccttacctatttcgctttatttaatatgtacaaatccggattttcttaaaagtgcggtcagttttcacgatgaatttaaaatatccgcaaaatccaccgcacttcaacctatttaacggctaaaactgctataattccgccaattttttctacatatttgagaccggt Mask Tandem Repeat Region ================================================ accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Find is-nt database================================================ Query_seq: AA00809:AA00810|AA00809:AA00810:possible oxidoreductase:alcohol dehydrogenase:->->:555496..555765 270 accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00809:AA00810|AA00809:AA00810:possible oxidoreductase:alcohol dehydrogenase:->->:555496..555765 270 accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00809:AA00810|AA00809:AA00810:possible oxidoreductase:alcohol dehydrogenase:->->:555496..555765 270 accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 56 End: 160 ....................................................... I P Q L A T S K A S I K F E D H F S V A S K L F D P N T C I L Y L S M .............................................................................................................. accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00809:AA00810|AA00809:AA00810:possible oxidoreductase:alcohol dehydrogenase:->->:555496..555765 270 accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Intra-Species Hit: Count: 1 Min: 1 Max: 270 Len: 270 Subject: aact_AA00809_AA00810|possible oxidoreductase:alcohol dehydrogenase|POSITIVE:POSITIVE|[555496,555765]|270 HSP 1 e-value: 1.0E-151 bit: 535.0 Len: 270 Query Start:1 Query End:270 Subject Strand: POSITIVE Subject Start: 1 Subject End: 270 accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct accggtctcaaatatgtagaaaaaattggcggaattatagcagttttagccgttaaataggttgaagtgcggtggattttgcggatattttaaattcatcgtgaaaactgaccgcacttttaagaaaatccggatttgtacatattaaataaagcgaaataggtaaggggaatttttacattaattttaaacctattgaaaatatcaactttttatccttaaaataaacttaatcattaaaaaacttactcacttttacgaggaaaagct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCGGUCUCAAAUAUGUAGAAAAAAUUGGCGGAAUUAUAGCAGUUUUAGCCGUUAAAUAGGUUGAAGUGCGGUGGAUUUUGCGGAUAUUUUAAAUUCAUCGUGAAAACUGACCGCACUUUUAAGAAAAUCCGGAUUUGUACAUAUUAAAUAAAGCGAAAUAGGUAAGGGGAAUUUUUACAUUAAUUUUAAACCUAUUGAAAAUAUCAACUUUUUAUCCUUAAAAUAAACUUAAUCAUUAAAAAACUUACUCACUUUUACGAGGAAAAGCU ..........((((((((.(((...(((((((....((....)).....)))))))...((..((((((((((((.(((..(((..............)))..))).)).)))))))))).........))...))).))))))))......(((..(((((((....((((((........))))))..)))))))...................................................(((........))).....))) (-48.14) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUUUUCCUCGUAAAAGUGAGUAAGUUUUUUAAUGAUUAAGUUUAUUUUAAGGAUAAAAAGUUGAUAUUUUCAAUAGGUUUAAAAUUAAUGUAAAAAUUCCCCUUACCUAUUUCGCUUUAUUUAAUAUGUACAAAUCCGGAUUUUCUUAAAAGUGCGGUCAGUUUUCACGAUGAAUUUAAAAUAUCCGCAAAAUCCACCGCACUUCAACCUAUUUAACGGCUAAAACUGCUAUAAUUCCGCCAAUUUUUUCUACAUAUUUGAGACCGGU ........(((...((((((((((((.((((((.(((((...((((((.....))))))..(((((.....)))))........)))))...)))))).....))))).....)))))))....((((((((.(((..((((.........(((((((((..(((((...((((.........))))...)))))..))))))))).............(((.......))).....)))).......))).)))))))).)))...... (-44.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 447 100 58 168 131 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 416 120 80 150 114 aact:AA02084|5end_conserved_hypothetical_protein_1417843..1418073_POSITIVE 407 120 71 176 127 aact:AA01564|5end_transaldolase_B_1049099..1049329_POSITIVE 399 120 71 136 86 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 393 84 41 223 181 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 386 120 80 156 120 aact:AA00473|5end_tryptophanyl-tRNA_synthetase_328996..329226_POSITIVE 379 133 96 68 37 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 377 130 100 172 143 aact:AA02927|5end_hypothetical_protein_2048233..2048463_POSITIVE 377 130 100 75 46 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 365 120 72 226 179 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 358 83 46 114 72 aact:AA00186|5end_o-succinylbenzoate-CoA_synthase;_OSB_synthase_131367..131597_POSITIVE 357 125 81 176 133 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 354 100 60 92 45 aact:AA00264|5end_hypothetical_protein_185052..185282_POSITIVE 351 138 95 185 143 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 351 120 72 122 66 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 350 120 74 201 156 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 350 120 74 48 3 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 341 84 41 71 35 aact:AA00130|5end_mercuric_transport_protein_(periplasmic_mercury_ion_binding_protein)_(mercury_scavenger_protein)_94217..94447_POSITIVE 340 120 80 188 150 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 339 110 62 209 160 aact:AA02743|5end_outer_membrane_lipoprotein_precursor/glutamyl-tRNA_reductase_subunit_1922650..1922880_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 447 100 58 150 113 aact:AA01582|3end_hypothetical_protein_1065651..1065801_POSITIVE 430 120 71 76 23 aact:AA01351|3end_probable_sulfatase_916393..916543_POSITIVE 416 120 80 75 39 aact:AA02082|3end_glutaminyl-tRNA_synthetase_1417848..1417998_POSITIVE 399 120 71 149 99 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 393 84 41 139 97 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 386 120 80 63 27 aact:AA00471|3end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328983..329133_POSITIVE 379 133 96 96 65 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 357 125 81 84 41 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 354 100 60 55 8 aact:AA00263|3end_ATP-dependent_RNA_helicase_185095..185245_POSITIVE 342 84 42 76 30 aact:AA02747|3end_ribose-phosphate_pyrophosphokinase;_phosphoribosylpyrophosphate_synthase_1925258..1925408_POSITIVE 340 120 80 84 46 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 339 120 74 115 67 aact:AA01492|3end_glucose_inhibited_division_protein_B_1003566..1003716_POSITIVE 336 120 72 71 19 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 334 120 71 120 76 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 333 90 45 74 31 aact:AA01451|3end_D-xylulokinase_973484..973634_POSITIVE 331 120 71 71 21 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 328 130 81 78 32 aact:AA01179|3end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_808801..808951_POSITIVE 326 120 71 74 28 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 324 120 81 48 9 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 322 76 31 57 14 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE