Origin IGS: taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaaaacgcgcaaaaaagagaccgcactttttggtaaaacaatgatgacagacataaaaaacgccccgcattgagagcgttttttgttgctatttgggtta Mask Tandem Repeat Region ================================================ taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Find is-nt database================================================ Query_seq: AA00807:AA00809|AA00807:AA00809:fumarate reductase 13 kD hydrophobic protein:possible oxidoreductase:->->:553249..553350 102 taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00807:AA00809|AA00807:AA00809:fumarate reductase 13 kD hydrophobic protein:possible oxidoreductase:->->:553249..553350 102 taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00807:AA00809|AA00807:AA00809:fumarate reductase 13 kD hydrophobic protein:possible oxidoreductase:->->:553249..553350 102 taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 9 End: 101 ........ M A T K N A L N A G R F L C L S S L F Y Q K V R S L F C A F S . taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00807:AA00809|AA00807:AA00809:fumarate reductase 13 kD hydrophobic protein:possible oxidoreductase:->->:553249..553350 102 taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: aact_AA00807_AA00809|fumarate reductase 13 kD hydrophobic protein:possible oxidoreductase|POSITIVE:POSITIVE|[553249,553350]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta taacccaaatagcaacaaaaaacgctctcaatgcggggcgttttttatgtctgtcatcattgttttaccaaaaagtgcggtctcttttttgcgcgttttcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCCAAAUAGCAACAAAAAACGCUCUCAAUGCGGGGCGUUUUUUAUGUCUGUCAUCAUUGUUUUACCAAAAAGUGCGGUCUCUUUUUUGCGCGUUUUCUA ........((((((..((((((((((((......)))))))))))).)).))))....................((((((........))))))........ (-21.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAAACGCGCAAAAAAGAGACCGCACUUUUUGGUAAAACAAUGAUGACAGACAUAAAAAACGCCCCGCAUUGAGAGCGUUUUUUGUUGCUAUUUGGGUUA ........((.((((......((((.......))))..............((..(((((((((((.(........).)))))))))))..)).)))).)).. (-16.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 364 100 51 170 121 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 352 99 53 176 118 aact:AA01385|5end_50S_ribosomal_protein_L9_935509..935739_POSITIVE 339 43 3 72 34 aact:AA00994|5end_sodium/glutamate_symport_carrier_protein_(glutamate_permease)_695088..695318_POSITIVE 337 99 52 156 103 aact:AA01559|5end_RecA_regulatory_protein,_RecX_1045970..1046200_POSITIVE 336 64 23 154 119 aact:AA02635|5end_conserved_hypothetical_protein_1833678..1833908_POSITIVE 332 80 34 176 132 aact:AA00039|5end_cytochrome_c-type_biogenesis_protein_30312..30542_POSITIVE 331 80 35 185 139 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 331 99 58 189 151 aact:AA01401|5end_3-deoxy-D-manno-octulosonic-acid_transferase_946165..946395_POSITIVE 331 99 51 121 69 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 329 99 68 214 183 aact:AA02053|5end_acetyl-CoA_carboxylase_carboxyltransferase,_beta_1398928..1399158_POSITIVE 322 99 52 97 48 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 321 99 60 183 140 aact:AA02915|5end_thioredoxin_m_2040652..2040882_POSITIVE 311 59 12 174 123 aact:AA02357|5end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1614668..1614898_POSITIVE 306 99 51 209 145 aact:AA01284|5end_heat_shock_protein_(hsp60)_868574..868804_POSITIVE 306 99 62 63 27 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 303 99 51 205 154 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 302 99 69 129 99 aact:AA02418|5end_peptidyl-tRNA_hydrolase_1661090..1661320_POSITIVE 301 100 68 122 87 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 301 100 68 53 18 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 298 66 21 100 47 aact:AA02402|5end_O-sialoglycoprotein_endopeptidase_1651337..1651567_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 364 100 51 89 40 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 352 99 53 127 69 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 337 99 52 71 18 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 331 80 35 47 1 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 329 99 68 115 84 aact:AA02052|3end_probable_exodeoxyribonuclease_VII_small_subunit_1398909..1399059_POSITIVE 327 53 23 151 123 aact:AA02634|3end_rhamnosyltransferase;_glycosyltransferase_homolog_2_1833762..1833912_POSITIVE 322 99 52 82 33 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 311 59 12 82 31 aact:AA02356|3end_hypothetical_protein_1614656..1614806_POSITIVE 306 99 51 85 21 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 306 99 62 45 9 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 303 99 51 69 18 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 302 100 52 145 100 aact:AA02990|3end_beta_hexosaminadase_2090596..2090746_POSITIVE 298 66 21 74 21 aact:AA02400|3end_hypothetical_protein_1651391..1651541_POSITIVE 298 99 56 85 34 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 295 99 52 115 70 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 293 99 51 86 38 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 293 98 51 149 108 aact:AA00003|3end_DNA_replication_and_repair_protein_3490..3640_POSITIVE 289 99 60 127 91 aact:AA01597|3end_oligoribonuclease_1078936..1079086_POSITIVE 285 99 67 81 51 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE 285 99 51 55 12 aact:AA01107|3end_adenylosuccinate_lyase_763754..763904_POSITIVE