Origin IGS: taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgccaaataatgtttttactcgaattgatagctttctgaatgaatttttaacgggtattacagggcatattcagaaattgttatcgttggggtgggattattcgatagtttacttgaaaccacttctgacttttcagatgttgttggaagagttggaaagtagaattgcctatagcaacactaagttgtattggttttaaattaatggtgattccaattttaagtcatttattttagattttaaaataaataagaatta Mask Tandem Repeat Region ================================================ taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Find is-nt database================================================ Query_seq: AA00780:AA00781|AA00780:AA00781:monofunctional biosynthetic peptidoglycan transglycosylase:hypothetical protein:->->:537765..538026 262 taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00780:AA00781|AA00780:AA00781:monofunctional biosynthetic peptidoglycan transglycosylase:hypothetical protein:->->:537765..538026 262 taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00780:AA00781|AA00780:AA00781:monofunctional biosynthetic peptidoglycan transglycosylase:hypothetical protein:->->:537765..538026 262 taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 42 End: 176 ......................................... M G I T I N L K P I Q L S V A I G N S T F Q L F Q Q H L K S Q K W F Q V N Y R I I P P Q R ...................................................................................... Protein_Len: 39 Strand: - Start: 100 End: 216 ................................................................................................... K G V R G V V D S F D S T T E L L S D F L G V G V I V I E S Y A R Y Y G N F M .............................................. Protein_Len: 60 Strand: - Start: 63 End: 260 .............................................................. N S Y A I R S E L E E L L M Q F T L L P K L Y V I S Y D W G L S L L K Q I H G T I G T L F E N L F S D I R T F V N N P M .. taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 14 End: 43 .............TTTTAAAATCTAAAATAAATGACTTAAAAT........................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 48 HP_score: -3.4 Tail_Score: -3.43033 Start: 72 End: 84 Full_Region: tagaattgcctatag caac actaa gttg tattggttttaaatt .......................................................................caacactaagttg.................................................................................................................................................................................. Find igs database================================================ Query_seq: AA00780:AA00781|AA00780:AA00781:monofunctional biosynthetic peptidoglycan transglycosylase:hypothetical protein:->->:537765..538026 262 taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Intra-Species Hit: Count: 1 Min: 1 Max: 262 Len: 262 Subject: aact_AA00780_AA00781|monofunctional biosynthetic peptidoglycan transglycosylase:hypothetical protein|POSITIVE:POSITIVE|[537765,538026]|262 HSP 1 e-value: 1.0E-146 bit: 519.0 Len: 262 Query Start:1 Query End:262 Subject Strand: POSITIVE Subject Start: 1 Subject End: 262 taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta taattcttatttattttaaaatctaaaataaatgacttaaaattggaatcaccattaatttaaaaccaatacaacttagtgttgctataggcaattctactttccaactcttccaacaacatctgaaaagtcagaagtggtttcaagtaaactatcgaataatcccaccccaacgataacaatttctgaatatgccctgtaatacccgttaaaaattcattcagaaagctatcaattcgagtaaaaacattatttggcatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCUUAUUUAUUUUAAAAUCUAAAAUAAAUGACUUAAAAUUGGAAUCACCAUUAAUUUAAAACCAAUACAACUUAGUGUUGCUAUAGGCAAUUCUACUUUCCAACUCUUCCAACAACAUCUGAAAAGUCAGAAGUGGUUUCAAGUAAACUAUCGAAUAAUCCCACCCCAACGAUAACAAUUUCUGAAUAUGCCCUGUAAUACCCGUUAAAAAUUCAUUCAGAAAGCUAUCAAUUCGAGUAAAAACAUUAUUUGGCAUUA ..((((.(((((((((((.....)))))))))))..(((...((((((((((........................(((.(((((.....))))).)))......................(((((....))))).)))))))))).)))......))))..............((((....(((((((((........((((....)))).......)))))))))..))))...((((((((.....))))))))..... (-43.06) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGCCAAAUAAUGUUUUUACUCGAAUUGAUAGCUUUCUGAAUGAAUUUUUAACGGGUAUUACAGGGCAUAUUCAGAAAUUGUUAUCGUUGGGGUGGGAUUAUUCGAUAGUUUACUUGAAACCACUUCUGACUUUUCAGAUGUUGUUGGAAGAGUUGGAAAGUAGAAUUGCCUAUAGCAACACUAAGUUGUAUUGGUUUUAAAUUAAUGGUGAUUCCAAUUUUAAGUCAUUUAUUUUAGAUUUUAAAAUAAAUAAGAAUUA ................((((((((.(((.((((((((((((((((..(((.(((......))).)))...))))))))))..))))))))).))))))))..((((........((((((((..(((((..(((((((.(((...))).)))))))..).)))).((((..((....(((((.....))))).(((((.....))))).))..))))...)))))))).(((((((((((...)))))))))))..)))).. (-57.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 125 81 47 1 aact:AA00900|5end_conserved_hypothetical_protein_623442..623672_POSITIVE 272 195 152 210 158 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 269 197 152 224 174 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 268 177 133 215 156 aact:AA01209|5end_conserved_hypothetical_protein_826430..826660_POSITIVE 268 177 133 73 14 aact:AA01207|5end_hypothetical_protein_826288..826518_POSITIVE 263 198 154 152 106 aact:AA00199|5end_hypothetical_protein_138575..138805_POSITIVE 250 210 167 78 36 aact:AA02260|5end_pseudouridylate_synthase_I_1537067..1537297_POSITIVE 249 116 78 229 183 aact:AA00931|5end_elongation_factor_Tu_(tufA)_641920..642150_POSITIVE 243 239 194 60 6 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 241 195 155 155 122 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 237 229 186 65 22 aact:AA00185|5end_3-dehydroquinate_dehydratase_II_130792..131022_POSITIVE 234 107 66 231 189 aact:AA02844|5end_Holliday_junction_DNA_helicase_1996942..1997172_POSITIVE 229 176 136 220 185 aact:AA01670|5end_conserved_hypothetical_protein_1130382..1130612_POSITIVE 228 169 123 220 171 aact:AA02558|5end_conserved_hypothetical_protein_1780930..1781160_POSITIVE 227 118 76 217 163 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 227 200 156 158 119 aact:AA01867|5end_thiamine_transport_ATP-binding_protein_1254853..1255083_POSITIVE 224 259 231 58 30 aact:AA00811|5end_conserved_hypothetical_protein_556736..556966_POSITIVE 222 176 136 118 65 aact:AA00890|5end_conserved_hypothetical_protein_617075..617305_POSITIVE 221 199 155 61 6 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 221 209 168 68 18 aact:AA01919|5end_conserved_hypothetical_protein_1292719..1292949_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 263 198 154 140 94 aact:AA00198|3end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138643..138793_POSITIVE 257 148 107 132 96 aact:AA00716|3end_conserved_hypothetical_protein_491244..491394_POSITIVE 252 223 188 137 97 aact:AA00314|3end_conserved_hypothetical_protein_(possible_Kmt1_protein)_214326..214476_POSITIVE 250 210 167 59 17 aact:AA02259|3end_possible_RelE-like_protein_1537128..1537278_POSITIVE 237 239 196 49 1 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 237 229 186 104 61 aact:AA00184|3end_hypothetical_protein_130911..131061_POSITIVE 229 176 136 141 106 aact:AA01668|3end_transcription_elongation_factor_1130383..1130533_POSITIVE 222 210 162 96 49 aact:AA01911|3end_hypothetical_protein_1288284..1288434_POSITIVE 222 176 136 73 20 aact:AA00889|3end_conserved_hypothetical_protein_617110..617260_POSITIVE 219 159 115 108 59 aact:AA02084|3end_conserved_hypothetical_protein_1418354..1418504_POSITIVE 217 207 165 109 68 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 216 98 52 77 38 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 215 198 165 136 105 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 213 179 136 125 81 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 212 201 165 145 105 aact:AA02076|3end_conserved_hypothetical_protein_1411094..1411244_POSITIVE 210 209 163 137 95 aact:AA01477|3end_type_I_restriction_enzyme_R_protein_994479..994629_POSITIVE 210 175 134 59 25 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 209 169 121 77 20 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 209 237 195 69 25 aact:AA02568|3end_sugar_phosphotransferase_component_II_B_1788725..1788875_POSITIVE 209 191 155 151 122 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE