Origin IGS: taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgtgaccttcggtatgcgaattttacttctgtggataaatgaaaataattcgcgcaatgataatgaaattcatttcttttgcaagaaataaagataagttacattgagcttatttgatatatgtcatgattgttcagcttttgaaggaaataaacttcatcggtggaataggtcttatcataaagttatta Mask Tandem Repeat Region ================================================ taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Find is-nt database================================================ Query_seq: AA00761:AA00762|AA00761:AA00762:conserved hypothetical protein (possible peptidase):hemoglobin binding protein A:->->:524147..524338 192 taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00761:AA00762|AA00761:AA00762:conserved hypothetical protein (possible peptidase):hemoglobin binding protein A:->->:524147..524338 192 taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00761:AA00762|AA00761:AA00762:conserved hypothetical protein (possible peptidase):hemoglobin binding protein A:->->:524147..524338 192 taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 97 End: 192 ................................................................................................ M F I S C K R N E F H Y H C A N Y F H L S T E V K F A Y R R S H taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00761:AA00762|AA00761:AA00762:conserved hypothetical protein (possible peptidase):hemoglobin binding protein A:->->:524147..524338 192 taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Intra-Species Hit: Count: 1 Min: 1 Max: 192 Len: 192 Subject: aact_AA00761_AA00762|conserved hypothetical protein (possible peptidase):hemoglobin binding protein A|POSITIVE:POSITIVE|[524147,524338]|192 HSP 1 e-value: 1.0E-105 bit: 381.0 Len: 192 Query Start:1 Query End:192 Subject Strand: POSITIVE Subject Start: 1 Subject End: 192 taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat taataactttatgataagacctattccaccgatgaagtttatttccttcaaaagctgaacaatcatgacatatatcaaataagctcaatgtaacttatctttatttcttgcaaaagaaatgaatttcattatcattgcgcgaattattttcatttatccacagaagtaaaattcgcataccgaaggtcacat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAACUUUAUGAUAAGACCUAUUCCACCGAUGAAGUUUAUUUCCUUCAAAAGCUGAACAAUCAUGACAUAUAUCAAAUAAGCUCAAUGUAACUUAUCUUUAUUUCUUGCAAAAGAAAUGAAUUUCAUUAUCAUUGCGCGAAUUAUUUUCAUUUAUCCACAGAAGUAAAAUUCGCAUACCGAAGGUCACAU .................(((((........(((((((((((((((.......((((.........................))))...(((((..............)))))...)))))))))))))))........(((((((..((((...........))))...))))))).......))))).... (-33.75) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGUGACCUUCGGUAUGCGAAUUUUACUUCUGUGGAUAAAUGAAAAUAAUUCGCGCAAUGAUAAUGAAAUUCAUUUCUUUUGCAAGAAAUAAAGAUAAGUUACAUUGAGCUUAUUUGAUAUAUGUCAUGAUUGUUCAGCUUUUGAAGGAAAUAAACUUCAUCGGUGGAAUAGGUCUUAUCAUAAAGUUAUUA (((.((((........(((((((....(((...........)))...)))))))...((((((.........(((((((....)))))))..(((((((((......)))))))))......))))))...(((((.(((..(((((........)))))..))).)))))))))....))).......... (-39.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 64 21 176 130 aact:AA02727|5end_hypothetical_protein_1909735..1909965_POSITIVE 234 58 13 192 147 aact:AA01962|5end_recombination_protein_rec2_1326200..1326430_POSITIVE 233 190 147 140 99 aact:AA00876|5end_tight_adherence_protein_C_607761..607991_POSITIVE 229 58 13 226 177 aact:AA02545|5end_conserved_hypothetical_protein_1773965..1774195_POSITIVE 229 65 26 155 108 aact:AA01947|5end_iron(III)-transport_ATP-binding_protein_1316390..1316620_POSITIVE 229 189 158 71 37 aact:AA01172|5end_conserved_hypothetical_protein_803516..803746_POSITIVE 228 176 131 117 63 aact:AA00530|5end_hypothetical_protein_362373..362603_POSITIVE 227 58 11 149 100 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 226 77 31 183 119 aact:AA02383|5end_oxidoreductase_1631255..1631485_POSITIVE 219 176 136 101 63 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 218 90 53 130 89 aact:AA00199|5end_hypothetical_protein_138575..138805_POSITIVE 217 59 14 86 30 aact:AA02193|5end_oxaloacetate_decarboxylase_gamma_chain_1494219..1494449_POSITIVE 214 59 26 173 147 aact:AA00784|5end_hypothetical_protein_539025..539255_POSITIVE 213 70 24 138 77 aact:AA01782|5end_molybdenum_transport_protein;_transcriptional_regulator_1202879..1203109_POSITIVE 212 162 124 205 166 aact:AA02723|5end_hypothetical_protein_1906263..1906493_POSITIVE 210 116 84 196 164 aact:AA00684|5end_adenylosuccinate_synthase_467486..467716_POSITIVE 208 78 37 55 4 aact:AA02684|5end_hydrogenase_4_membrane_subunit_1869514..1869744_POSITIVE 208 49 6 102 50 aact:AA01804|5end_sugar_nucleotide_epimerase;_cell_division_inhibitor_1215037..1215267_POSITIVE 208 140 109 96 62 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 204 180 137 78 33 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 64 21 83 37 aact:AA02726|3end_anaerobic_C4-dicarboxylate_transporter_1909722..1909872_POSITIVE 234 58 13 142 97 aact:AA01961|3end_lipid_A_export_ATP-binding/permease_protein_msbA_1326230..1326380_POSITIVE 233 190 147 140 99 aact:AA00875|3end_tight_adherence_protein_B_607841..607991_POSITIVE 229 58 13 126 77 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 229 189 158 55 21 aact:AA01171|3end_LysA_activator_protein_803580..803730_POSITIVE 227 58 11 140 91 aact:AA02523|3end_conserved_hypothetical_protein_1753384..1753534_POSITIVE 218 90 53 118 77 aact:AA00198|3end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138643..138793_POSITIVE 212 49 6 107 57 aact:AA00981|3end_carboxylesterase_686377..686527_POSITIVE 210 116 84 105 73 aact:AA00682|3end_S-adenosylmethionine-dependent_methyltransferase_467475..467625_POSITIVE 208 49 6 75 23 aact:AA01803|3end_arginine_repressor_1215090..1215240_POSITIVE 208 140 109 96 62 aact:AA01755|3end_cobalt_membrane_transport_protein_1186555..1186705_POSITIVE 204 180 137 66 21 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 204 186 153 81 49 aact:AA00156|3end_hypothetical_protein_112102..112252_POSITIVE 203 165 121 117 81 aact:AA02465|3end_glucosamine-6-phosphate_isomerase_1704885..1705035_POSITIVE 197 177 136 140 92 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 196 48 2 116 74 aact:AA01244|3end_transposase_848093..848243_POSITIVE 195 179 135 116 72 aact:AA02512|3end_O-succinylbenzoate--CoA_ligase_1746871..1747021_POSITIVE 195 48 28 110 87 aact:AA00924|3end_hypothetical_protein_635457..635607_POSITIVE 193 65 25 66 21 aact:AA02879|3end_octaprenyl-diphosphate_synthase_2020589..2020739_POSITIVE 193 90 44 112 58 aact:AA01136|3end_hypothetical_protein_778502..778652_POSITIVE