Origin IGS: tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tggtcttgctttcctctgttaattttcaagatggctatatgatacgccgattttgcctaactggtctaacgttataattaatgcttgcaatattctgatttcatagtaaattgcctttcagctaacatatgtaagtcgaatttccctctaacagattaaggaacagaga Mask Tandem Repeat Region ================================================ tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Find is-nt database================================================ Query_seq: AA00759:AA00760|AA00759:AA00760:3-oxoacyl-[acyl-carrier-protein] synthase I:conserved hypothetical protein (possible peptidase):->->:521961..522129 169 tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00759:AA00760|AA00759:AA00760:3-oxoacyl-[acyl-carrier-protein] synthase I:conserved hypothetical protein (possible peptidase):->->:521961..522129 169 tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00759:AA00760|AA00759:AA00760:3-oxoacyl-[acyl-carrier-protein] synthase I:conserved hypothetical protein (possible peptidase):->->:521961..522129 169 tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 59 End: 148 .......................................................... M Y Y E I R I L Q A L I I T L D Q L G K I G V S Y S H L E N ..................... tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00759:AA00760|AA00759:AA00760:3-oxoacyl-[acyl-carrier-protein] synthase I:conserved hypothetical protein (possible peptidase):->->:521961..522129 169 tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Intra-Species Hit: Count: 1 Min: 1 Max: 169 Len: 169 Subject: aact_AA00759_AA00760|3-oxoacyl-[acyl-carrier-protein] synthase I:conserved hypothetical protein (possible peptidase)|POSITIVE:POSITIVE|[521961,522129]|169 HSP 1 e-value: 3.0E-91 bit: 335.0 Len: 169 Query Start:1 Query End:169 Subject Strand: POSITIVE Subject Start: 1 Subject End: 169 tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca tctctgttccttaatctgttagagggaaattcgacttacatatgttagctgaaaggcaatttactatgaaatcagaatattgcaagcattaattataacgttagaccagttaggcaaaatcggcgtatcatatagccatcttgaaaattaacagaggaaagcaagacca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUCUGUUCCUUAAUCUGUUAGAGGGAAAUUCGACUUACAUAUGUUAGCUGAAAGGCAAUUUACUAUGAAAUCAGAAUAUUGCAAGCAUUAAUUAUAACGUUAGACCAGUUAGGCAAAAUCGGCGUAUCAUAUAGCCAUCUUGAAAAUUAACAGAGGAAAGCAAGACCA ((((((((((((.........))))....(((((.....(((((...(((((...(((((......((....))....)))))..((.((((((.............))))))))....)))))....))))).......)))))....))))))))............ (-28.92) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGUCUUGCUUUCCUCUGUUAAUUUUCAAGAUGGCUAUAUGAUACGCCGAUUUUGCCUAACUGGUCUAACGUUAUAAUUAAUGCUUGCAAUAUUCUGAUUUCAUAGUAAAUUGCCUUUCAGCUAACAUAUGUAAGUCGAAUUUCCCUCUAACAGAUUAAGGAACAGAGA ...((((...(((((((((((.....(((((((((..........))).)))))).......((.....(((((....)))))........((((.(((((((((.......((......)).....)))).)))))))))...))..)))))))....))))..)))) (-28.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 317 149 106 69 19 aact:AA01499|5end_fructose_1,6-bisphosphatase_protein_1008357..1008587_POSITIVE 307 87 44 90 44 aact:AA02955|5end_trigger_factor_2067022..2067252_POSITIVE 286 128 81 114 69 aact:AA02103|5end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431105..1431335_POSITIVE 285 138 99 45 1 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 262 59 14 144 96 aact:AA01394|5end_transcriptional_regulator_(mtr_efflux_pump)_942301..942531_POSITIVE 255 126 81 116 76 aact:AA00279|5end_8-amino-7-oxonoanoate_synthase_195262..195492_POSITIVE 249 127 98 227 199 aact:AA01615|5end_lipoprotein_releasing_system_transmembrane_1091186..1091416_POSITIVE 248 125 97 138 109 aact:AA01655|5end_hypothetical_protein_1121187..1121417_POSITIVE 248 136 99 95 46 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 247 116 73 166 124 aact:AA02628|5end_exodeoxyribonuclease_III_1829125..1829355_POSITIVE 247 100 55 76 31 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 247 140 113 29 5 aact:AA00106|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase_75544..75774_POSITIVE 246 144 103 171 132 aact:AA01248|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849734..849964_POSITIVE 246 144 103 101 62 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 244 137 98 209 171 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 242 158 114 166 103 aact:AA00003|5end_DNA_replication_and_repair_protein_2337..2567_POSITIVE 240 137 105 151 122 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 240 160 112 119 64 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 239 49 12 200 163 aact:AA02775|5end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_(Queuosine_biosynthesis_protein_queA)_1938555..1938785_POSITIVE 238 130 82 130 78 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 286 128 81 117 72 aact:AA02101|3end_penicillin-binding_protein_7_1431188..1431338_POSITIVE 275 160 111 97 33 aact:AA01276|3end_heptosyltransferase_III_865844..865994_POSITIVE 255 126 81 132 92 aact:AA00278|3end_conserved_hypothetical_protein_195358..195508_POSITIVE 248 125 97 34 5 aact:AA01654|3end_periplasmic_maltose-binding_protein_precursor_1121163..1121313_POSITIVE 248 136 99 84 35 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 247 100 55 102 57 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 242 128 83 151 105 aact:AA00983|3end_aldo-keto_reductase_688492..688642_POSITIVE 238 130 82 127 75 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 234 159 113 43 1 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 232 126 81 65 32 aact:AA00845|3end_hypothetical_protein_584730..584880_POSITIVE 231 139 99 75 43 aact:AA00291|3end_hypothetical_protein_201905..202055_POSITIVE 230 140 99 41 5 aact:AA00969|3end_conserved_hypothetical_protein_(possible_NADH_pyrophosphatase)_676206..676356_POSITIVE 229 123 81 66 15 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 229 136 91 128 87 aact:AA01502|3end_serine_acetyltransferase_1010888..1011038_POSITIVE 229 126 95 43 10 aact:AA01419|3end_D-ribulose-phosphate-3_epimerase_955129..955279_POSITIVE 229 59 14 143 91 aact:AA00196|3end_conserved_hypothetical_protein_138015..138165_POSITIVE 227 136 98 55 13 aact:AA01805|3end_electron_transport_complex_protein_1216773..1216923_POSITIVE 227 125 99 145 114 aact:AA00165|3end_conserved_hypothetical_protein_117647..117797_POSITIVE 226 59 16 51 1 aact:AA02470|3end_glucose_kinase_1707694..1707844_POSITIVE 226 138 91 106 58 aact:AA01666|3end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1129621..1129771_POSITIVE