Origin IGS: aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaacgcttactttaaaaattgccgggttcgtcacaaaattttccgttttctgacgtgtttttacgataaagcatgggcgaaatgtatttttgtgatcccgctaacagtttatttcgttaaatatggtatggtatgacatattttttgtaatcgttttcataagctaaaaatcaatttatcgaggcacatt Mask Tandem Repeat Region ================================================ aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Find is-nt database================================================ Query_seq: AA00747:AA00748|AA00747:AA00748:UTP--hexose-1-phosphate uridylyltransferase / UDPglucose-hexose-1-phosphate uridylyltransferas:hypothetical protein:->->:513565..513757 193 aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00747:AA00748|AA00747:AA00748:UTP--hexose-1-phosphate uridylyltransferase / UDPglucose-hexose-1-phosphate uridylyltransferas:hypothetical protein:->->:513565..513757 193 aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00747:AA00748|AA00747:AA00748:UTP--hexose-1-phosphate uridylyltransferase / UDPglucose-hexose-1-phosphate uridylyltransferas:hypothetical protein:->->:513565..513757 193 aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 84 End: 176 ................................................................................... M L A G S Q K Y I S P M L Y R K N T S E N G K F C D E P G N F ................. Protein_Len: 32 Strand: - Start: 58 End: 153 ......................................................... V M G Y K V F Y V T L P I V F I C K A W A K D Y F C T L F R F M ........................................ aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00747:AA00748|AA00747:AA00748:UTP--hexose-1-phosphate uridylyltransferase / UDPglucose-hexose-1-phosphate uridylyltransferas:hypothetical protein:->->:513565..513757 193 aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Intra-Species Hit: Count: 1 Min: 1 Max: 193 Len: 193 Subject: aact_AA00747_AA00748|UTP--hexose-1-phosphate uridylyltransferase / UDPglucose-hexose-1-phosphate uridylyltransferas:hypothetical protein|POSITIVE:POSITIVE|[513565,513757]|193 HSP 1 e-value: 1.0E-105 bit: 383.0 Len: 193 Query Start:1 Query End:193 Subject Strand: POSITIVE Subject Start: 1 Subject End: 193 aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca aatgtgcctcgataaattgatttttagcttatgaaaacgattacaaaaaatatgtcataccataccatatttaacgaaataaactgttagcgggatcacaaaaatacatttcgcccatgctttatcgtaaaaacacgtcagaaaacggaaaattttgtgacgaacccggcaatttttaaagtaagcgttttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGUGCCUCGAUAAAUUGAUUUUUAGCUUAUGAAAACGAUUACAAAAAAUAUGUCAUACCAUACCAUAUUUAACGAAAUAAACUGUUAGCGGGAUCACAAAAAUACAUUUCGCCCAUGCUUUAUCGUAAAAACACGUCAGAAAACGGAAAAUUUUGUGACGAACCCGGCAAUUUUUAAAGUAAGCGUUUUCA ...............................((((((((.((((.((((((.((((.............(((.((((..((((..((..(((..((...........))..)))..))..)))))))).)))...(((((.((((.......)))).))))).....))))))))))...)))).)))))))) (-33.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAACGCUUACUUUAAAAAUUGCCGGGUUCGUCACAAAAUUUUCCGUUUUCUGACGUGUUUUUACGAUAAAGCAUGGGCGAAAUGUAUUUUUGUGAUCCCGCUAACAGUUUAUUUCGUUAAAUAUGGUAUGGUAUGACAUAUUUUUUGUAAUCGUUUUCAUAAGCUAAAAAUCAAUUUAUCGAGGCACAUU ((((((((.((((.....((((((.((((...(((((((((.....((((((....((((((((......))))))))...))))))...)))))))))))))....))))))..............((((((......))))))....)))).))))))))...(((.................)))..... (-42.63) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 127 84 213 168 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 283 188 145 200 163 aact:AA01039|5end_transcriptional_regulator_(possible_murein_gene_regulator)_723864..724094_POSITIVE 283 188 145 55 18 aact:AA01037|5end_hypothetical_protein_723719..723949_POSITIVE 268 123 83 228 176 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 259 190 146 75 24 aact:AA01097|5end_conserved_hypothetical_protein_757189..757419_POSITIVE 258 120 75 59 7 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 252 188 146 72 27 aact:AA01959|5end_tetraacyldisaccharide_4'-kinase_1323412..1323642_POSITIVE 252 128 85 164 115 aact:AA00612|5end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_417840..418070_POSITIVE 251 170 126 58 1 aact:AA02749|5end_L-lactate_dehydrogenase_1925814..1926044_POSITIVE 248 128 84 218 176 aact:AA02575|5end_transmembrane_protein_1793749..1793979_POSITIVE 248 149 106 228 191 aact:AA02416|5end_conserved_hypothetical_protein_1658920..1659150_POSITIVE 246 154 113 92 44 aact:AA00190|5end_triosephosphate_isomerase_134088..134318_POSITIVE 244 172 136 164 127 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 243 151 112 36 1 aact:AA00886|5end_hypothetical_protein_616584..616814_POSITIVE 242 147 107 47 10 aact:AA01928|5end_ATP-dependent_RNA_helicase_1301986..1302216_POSITIVE 241 127 81 148 88 aact:AA00521|5end_phosphatase_357744..357974_POSITIVE 239 147 111 190 151 aact:AA00545|5end_glutathionylspermidine_synthetase;amidase_373607..373837_POSITIVE 238 187 146 81 40 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 238 188 146 37 1 aact:AA00148|5end_ATP-dependent_RNA_helicase_105955..106185_POSITIVE 237 128 84 176 132 aact:AA00725|5end_translation_initiation_factor_1_497981..498211_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 127 84 118 73 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 259 190 146 88 37 aact:AA01096|3end_conserved_hypothetical_protei_757282..757432_POSITIVE 258 120 75 112 60 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 252 188 146 47 2 aact:AA01957|3end_conserved_hypothetical_protein_1323467..1323617_POSITIVE 252 128 85 140 91 aact:AA00611|3end_(3R)-hydroxymyristoyl-acyl_carrier_protein_dehydrase_417896..418046_POSITIVE 248 149 106 149 112 aact:AA02414|3end_hypothetical_protein_1658921..1659071_POSITIVE 241 127 81 145 85 aact:AA00519|3end_O-acetyltransferase_357821..357971_POSITIVE 237 128 84 151 107 aact:AA00724|3end_hypothetical_protein_498036..498186_POSITIVE 235 162 126 70 35 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 234 149 105 68 29 aact:AA01962|3end_recombination_protein_rec2_1328706..1328856_POSITIVE 232 150 105 148 105 aact:AA00132|3end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_95299..95449_POSITIVE 230 150 107 150 104 aact:AA01948|3end_ABC_transporter_permease,_membrane_spanning_protein_1318196..1318346_POSITIVE 226 127 84 133 72 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 225 121 82 150 99 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 225 115 72 83 40 aact:AA00605|3end_CDP-diglyceride_pyrophosphorylase_411876..412026_POSITIVE 223 187 145 137 90 aact:AA02742|3end_tRNA_nucleotidyltransferase_1922698..1922848_POSITIVE 223 172 145 143 114 aact:AA01166|3end_sulphite_synthesis_pathway_protein_799485..799635_POSITIVE 223 168 132 132 90 aact:AA00585|3end_glycerol-3-phosphate_regulon_repressor_399778..399928_POSITIVE 221 99 51 137 90 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 220 148 104 60 12 aact:AA01682|3end_molybdate_ABC_transporter_protein_1136442..1136592_POSITIVE