Origin IGS: tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atatactccttaaatttatctgtattaaacgatatttagcggttagtaagtgaagatagaatcattttagttattctatatttttcttacaactaagctaaattgaacaggaattcccaccacgccataccgatagttaagtggacgattaagctgccgaaggcaatgatggcgccggcggtccatca Mask Tandem Repeat Region ================================================ tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Find is-nt database================================================ Query_seq: AA00741:AA00742|AA00741:AA00742:possible 2-oxoglutarate/malate transporter:hypothetical protein:->->:508137..508324 188 tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00741:AA00742|AA00741:AA00742:possible 2-oxoglutarate/malate transporter:hypothetical protein:->->:508137..508324 188 tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00741:AA00742|AA00741:AA00742:possible 2-oxoglutarate/malate transporter:hypothetical protein:->->:508137..508324 188 tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 3 End: 110 .. M D R R R H H C L R Q L N R P L N Y R Y G V V G I P V Q F S L V V R K I .............................................................................. tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 96 End: 125 ...............................................................................................GTTGTAAGAAAAATATAGAATAACTAAAAT............................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00741:AA00742|AA00741:AA00742:possible 2-oxoglutarate/malate transporter:hypothetical protein:->->:508137..508324 188 tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Intra-Species Hit: Count: 1 Min: 1 Max: 188 Len: 188 Subject: aact_AA00741_AA00742|possible 2-oxoglutarate/malate transporter:hypothetical protein|POSITIVE:POSITIVE|[508137,508324]|188 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat tgatggaccgccggcgccatcattgccttcggcagcttaatcgtccacttaactatcggtatggcgtggtgggaattcctgttcaatttagcttagttgtaagaaaaatatagaataactaaaatgattctatcttcacttactaaccgctaaatatcgtttaatacagataaatttaaggagtatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUGGACCGCCGGCGCCAUCAUUGCCUUCGGCAGCUUAAUCGUCCACUUAACUAUCGGUAUGGCGUGGUGGGAAUUCCUGUUCAAUUUAGCUUAGUUGUAAGAAAAAUAUAGAAUAACUAAAAUGAUUCUAUCUUCACUUACUAACCGCUAAAUAUCGUUUAAUACAGAUAAAUUUAAGGAGUAUAU .......((((((.(((((...(((((...)))))....((((.............)))).)))))))))))..((((((.....(((((((...((((((((...((.(((((((..........))))))).))..))))).))).)))))))...((.....))...........)))))).... (-43.82) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUACUCCUUAAAUUUAUCUGUAUUAAACGAUAUUUAGCGGUUAGUAAGUGAAGAUAGAAUCAUUUUAGUUAUUCUAUAUUUUUCUUACAACUAAGCUAAAUUGAACAGGAAUUCCCACCACGCCAUACCGAUAGUUAAGUGGACGAUUAAGCUGCCGAAGGCAAUGAUGGCGCCGGCGGUCCAUCA .....(..(((((...((((.((((....((..(((((((((((.(((((.(((((((((((.((....)).))))))).)))).)))))))))..)))))))......((.....))....))..)))).)))).)))))..)..(((...(((((((..(.((....)).)..)))))))..))). (-45.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 466 37 4 47 15 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 430 49 1 105 42 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 400 44 4 55 9 aact:AA02334|5end_outer_membrane_protein_P1_1595307..1595537_POSITIVE 377 48 3 221 169 aact:AA00008|5end_hypothetical_protein_(possible_transposase_fragment)_5032..5262_POSITIVE 370 47 4 46 8 aact:AA02021|5end_anaerobic_glycerol-3-phosphate_dehydrogenase_1374935..1375165_POSITIVE 370 50 3 130 86 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 369 25 8 71 54 aact:AA02023|5end_NADH_dehydrogenase_1376701..1376931_POSITIVE 365 50 3 191 136 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 364 45 4 202 159 aact:AA00528|5end_conserved_hypothetical_protein_361396..361626_POSITIVE 363 45 4 95 45 aact:AA00652|5end_arginyl-tRNA_synthetase_(arginine--tRNA_ligase)_445718..445948_POSITIVE 361 48 7 217 176 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 360 47 7 90 39 aact:AA02756|5end_phosphoserine_aminotransferase_1929477..1929707_POSITIVE 358 50 8 127 66 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 357 47 8 58 26 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 354 50 5 75 15 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 353 80 32 212 170 aact:AA02551|5end_iron_(chelated)_ABC_transporter,_permease_protein_1777641..1777871_POSITIVE 353 45 9 188 137 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 353 44 1 101 45 aact:AA00512|5end_conserved_hypothetical_protein_354411..354641_POSITIVE 349 43 8 84 46 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 349 46 7 53 6 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 436 37 9 27 2 aact:AA01407|3end_outer_membrane_antigenic_lipoprotein_b_precursor_950251..950401_POSITIVE 404 47 1 118 69 aact:AA00741|3end_possible_2-oxoglutarate/malate_transporter_508076..508226_POSITIVE 369 25 8 44 27 aact:AA02022|3end_hypothetical_protein_1376754..1376904_POSITIVE 358 50 8 146 98 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 353 47 9 64 20 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 353 44 1 92 36 aact:AA00511|3end_nicotinamide_mononucleotide_transporter_354482..354632_POSITIVE 349 43 8 58 20 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 349 46 7 53 6 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 348 47 1 83 26 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 342 32 3 73 32 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 340 47 2 73 16 aact:AA00525|3end_hypothetical_protein_359459..359609_POSITIVE 336 48 3 105 48 aact:AA01614|3end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_1090719..1090869_POSITIVE 336 42 3 65 1 aact:AA01546|3end_deoxyribose_operon_repressor_1036320..1036470_POSITIVE 334 50 3 93 42 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 334 48 4 146 100 aact:AA01236|3end_50S_ribosomal_protein_L15_842970..843120_POSITIVE 334 70 24 71 35 aact:AA01202|3end_integrase/recombinase_824207..824357_POSITIVE 334 69 21 52 5 aact:AA00969|3end_conserved_hypothetical_protein_(possible_NADH_pyrophosphatase)_676206..676356_POSITIVE 330 75 32 139 92 aact:AA02368|3end_conserved_hypothetical_protein_1624286..1624436_POSITIVE 330 31 5 136 105 aact:AA00682|3end_S-adenosylmethionine-dependent_methyltransferase_467475..467625_POSITIVE 327 47 1 137 86 aact:AA02495|3end_conserved_hypothetical_protein_1731992..1732142_POSITIVE