Origin IGS: aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggatcgaacaataagccctttcgtgaaaacagaatggctttttaacagagaagtgtgctctgtttttttatattaatttaattatttaatgagagtaagttagcaaaaggttacaaaacttctacaaggaattttt Mask Tandem Repeat Region ================================================ aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Find is-nt database================================================ Query_seq: AA00721:AA00722|AA00721:AA00722:probable beta-N-acetylhexosaminidase (lacto-N-biosidase); dispersin B:nucleoside diphosphate kinase:->->:496022..496159 138 aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00721:AA00722|AA00721:AA00722:probable beta-N-acetylhexosaminidase (lacto-N-biosidase); dispersin B:nucleoside diphosphate kinase:->->:496022..496159 138 aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00721:AA00722|AA00721:AA00722:probable beta-N-acetylhexosaminidase (lacto-N-biosidase); dispersin B:nucleoside diphosphate kinase:->->:496022..496159 138 aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Predict ORF larger than 30AA ================================================ aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -5.1 Tail_Score: -5.82172 Start: 75 End: 91 Full_Region: agaatggctttttaa cagagaa gtg tgctctg tttttttatattaat ..........................................................................cagagaagtgtgctctg............................................... Find igs database================================================ Query_seq: AA00721:AA00722|AA00721:AA00722:probable beta-N-acetylhexosaminidase (lacto-N-biosidase); dispersin B:nucleoside diphosphate kinase:->->:496022..496159 138 aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: aact_AA00721_AA00722|probable beta-N-acetylhexosaminidase (lacto-N-biosidase); dispersin B:nucleoside diphosphate kinase|POSITIVE:POSITIVE|[496022,496159]|138 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatatnnnnnnncagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta aaaaattccttgtagaagttttgtaaccttttgctaacttactctcattaaataattaaattaatataaaaaaacagagcacacttctctgttaaaaagccattctgttttcacgaaagggcttattgttcgatccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAUUCCUUGUAGAAGUUUUGUAACCUUUUGCUAACUUACUCUCAUUAAAUAAUUAAAUUAAUAUAAAAAAACAGAGCACACUUCUCUGUUAAAAAGCCAUUCUGUUUUCACGAAAGGGCUUAUUGUUCGAUCCUA ...........(((((((.........)))))))............((..(((((.................(((((((.......)))))))...(((((.(((((....)).)))..))))).)))))..)).... (-19.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGAUCGAACAAUAAGCCCUUUCGUGAAAACAGAAUGGCUUUUUAACAGAGAAGUGUGCUCUGUUUUUUUAUAUUAAUUUAAUUAUUUAAUGAGAGUAAGUUAGCAAAAGGUUACAAAACUUCUACAAGGAAUUUUU ...((((.......(((((.((..((....))..)).)))))...(((((((.......)))))))........((((((((.((((...))))...)))))))).....))))..((((.((((....)))).)))) (-21.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 299 115 79 110 77 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 258 123 84 137 97 aact:AA01838|5end_conserved_hypothetical_protein_1236396..1236626_POSITIVE 243 123 82 199 161 aact:AA01568|5end_dipeptide_transport_system_ATP-binding_protein_1056037..1056267_POSITIVE 229 124 97 186 164 aact:AA01404|5end_hypothetical_protein_948578..948808_POSITIVE 222 128 87 217 171 aact:AA02271|5end_hypothetical_protein_1548623..1548853_POSITIVE 218 128 90 231 190 aact:AA00002|5end_DNA_polymerase_III,_beta_subunit_1233..1463_POSITIVE 217 128 86 162 124 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 217 113 79 86 49 aact:AA00902|5end_RNA_polymerase_sigma-E_factor_sigma-24_623829..624059_POSITIVE 215 47 17 128 93 aact:AA00033|5end_conserved_hypothetical_protein_25643..25873_POSITIVE 214 130 98 188 155 aact:AA00870|5end_rough_colony_protein_C_601735..601965_POSITIVE 212 106 69 176 137 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 212 128 85 63 28 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 212 48 5 109 62 aact:AA00110|5end_ATPase_involved_in_DNA_replication_79085..79315_POSITIVE 211 124 82 58 17 aact:AA02369|5end_conserved_hypothetical_protein_1624248..1624478_POSITIVE 210 130 85 85 35 aact:AA00081|5end_fatty_acid_metabolism_regulator_protein_59959..60189_POSITIVE 208 119 89 65 33 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 208 120 77 72 35 aact:AA00785|5end_conserved_hypothetical_protein_(possible_integrase)_539221..539451_POSITIVE 207 124 84 177 130 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 205 130 82 153 108 aact:AA02883|5end_conserved_hypothetical_protein_2021418..2021648_POSITIVE 204 129 96 42 2 aact:AA02610|5end_acyl-CoA_thioesterase_II_1820371..1820601_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 299 115 79 126 93 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 258 123 84 57 17 aact:AA01837|3end_transcriptional_regulatory_protein_1236396..1236546_POSITIVE 248 123 97 80 50 aact:AA02972|3end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077622..2077772_POSITIVE 225 126 87 149 104 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE 221 123 87 42 13 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 221 120 75 144 98 aact:AA00512|3end_conserved_hypothetical_protein_354649..354799_POSITIVE 219 123 82 143 83 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 217 128 86 67 29 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 216 124 99 33 4 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 215 47 17 122 87 aact:AA00031|3end_conserved_hypothetical_protein_25717..25867_POSITIVE 214 130 98 133 100 aact:AA00869|3end_tight_adherence_protein_V;_prepilin_peptidase_601760..601910_POSITIVE 212 48 5 97 50 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 208 120 77 82 45 aact:AA00784|3end_hypothetical_protein_539311..539461_POSITIVE 205 130 82 78 33 aact:AA02881|3end_50S_ribosomal_protein_L27_2021423..2021573_POSITIVE 204 122 84 70 31 aact:AA00164|3end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_117445..117595_POSITIVE 201 126 87 68 24 aact:AA01227|3end_hypothetical_protein_838845..838995_POSITIVE 200 126 89 91 54 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 199 116 79 52 15 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 197 119 76 87 45 aact:AA01135|3end_possible_cell_filamentation_protein_777664..777814_POSITIVE 196 108 79 85 55 aact:AA00944|3end_transcription_elongation_factor_655232..655382_POSITIVE