Origin IGS: taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attttatttctccaaaactaacaaatcggacgataaccgcgatctttcttaatttagaaaaagacaaaagtgcggtggaatttttcggtgttttgaaaaacatacagaaaagcgaccgcacttttatgttatta Mask Tandem Repeat Region ================================================ taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Find is-nt database================================================ Query_seq: AA00709:AA00711|AA00709:AA00711:metallocluster assembly (NifU protein):conserved hypothetical protein:->->:487899..488032 134 taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00709:AA00711|AA00709:AA00711:metallocluster assembly (NifU protein):conserved hypothetical protein:->->:487899..488032 134 taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00709:AA00711|AA00709:AA00711:metallocluster assembly (NifU protein):conserved hypothetical protein:->->:487899..488032 134 taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 8 End: 133 ....... M K V R S L F C M F F K T P K N S T A L L S F S K L R K I A V I V R F V S F G E I K . Protein_Len: 43 Strand: - Start: 1 End: 129 L L M F T R D S K Q I N K L V G F F E V A S K D K E L N L F I A T I T R N T L K P S M ..... taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00709:AA00711|AA00709:AA00711:metallocluster assembly (NifU protein):conserved hypothetical protein:->->:487899..488032 134 taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Intra-Species Hit: Count: 1 Min: 1 Max: 134 Len: 134 Subject: aact_AA00709_AA00711|metallocluster assembly (NifU protein):conserved hypothetical protein|POSITIVE:POSITIVE|[487899,488032]|134 HSP 1 e-value: 2.0E-70 bit: 266.0 Len: 134 Query Start:1 Query End:134 Subject Strand: POSITIVE Subject Start: 1 Subject End: 134 taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat taataacataaaagtgcggtcgcttttctgtatgtttttcaaaacaccgaaaaattccaccgcacttttgtctttttctaaattaagaaagatcgcggttatcgtccgatttgttagttttggagaaataaaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAACAUAAAAGUGCGGUCGCUUUUCUGUAUGUUUUUCAAAACACCGAAAAAUUCCACCGCACUUUUGUCUUUUUCUAAAUUAAGAAAGAUCGCGGUUAUCGUCCGAUUUGUUAGUUUUGGAGAAAUAAAAU .......(((((((((((((.((......))..(((((((........)))))))...))))))))))))).((((((((((((((.(((.....(((.......))).))).)))).))))))))))...... (-33.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUAUUUCUCCAAAACUAACAAAUCGGACGAUAACCGCGAUCUUUCUUAAUUUAGAAAAAGACAAAAGUGCGGUGGAAUUUUUCGGUGUUUUGAAAAACAUACAGAAAAGCGACCGCACUUUUAUGUUAUUA ..........(((..............))).((((((.......(((((......))))).....(((((((((((.(...(((((.((((((....))))))...))))).).)))))))))))..)))))). (-31.44) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 439 79 36 140 93 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 439 79 36 74 27 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 412 70 24 171 117 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 410 71 41 115 85 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 394 54 11 150 109 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 384 60 11 188 132 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 383 69 22 151 107 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 376 84 41 230 189 aact:AA00580|5end_conserved_hypothetical_protein_(possible_membrane_protein)_397003..397233_POSITIVE 374 69 23 205 156 aact:AA00296|5end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_202384..202614_POSITIVE 374 69 23 88 39 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 362 74 32 179 138 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 358 79 35 195 140 aact:AA02668|5end_gluconate_permease_1856758..1856988_POSITIVE 345 58 11 190 142 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 338 70 32 154 117 aact:AA01737|5end_exoribonuclease_II_1172647..1172877_POSITIVE 338 70 32 40 3 aact:AA01735|5end_hypothetical_protein_1172533..1172763_POSITIVE 338 49 8 153 111 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 337 79 46 46 6 aact:AA02667|5end_hypothetical_protein_1856609..1856839_POSITIVE 333 49 10 148 102 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 329 49 9 194 158 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 328 68 21 136 95 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 432 79 41 38 2 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE 410 71 41 134 104 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 383 69 22 146 102 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 376 84 41 102 61 aact:AA00579|3end_probable_DNA_recombination_protein_396955..397105_POSITIVE 374 69 23 79 30 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 362 74 32 72 31 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 353 60 11 146 88 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 345 58 11 109 61 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 341 58 13 54 1 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 338 70 32 141 104 aact:AA01735|3end_hypothetical_protein_1172714..1172864_POSITIVE 329 49 9 93 57 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 328 68 21 149 108 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 327 69 24 146 100 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 327 69 24 70 24 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 319 71 41 91 61 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 318 51 11 99 61 aact:AA00684|3end_adenylosuccinate_synthase_468861..469011_POSITIVE 317 100 51 118 73 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 314 48 5 106 65 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 312 49 7 87 49 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 311 48 10 144 106 aact:AA01474|3end_hypothetical_protein_990431..990581_POSITIVE