Origin IGS: taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttgtcctcaagtttgaattttatcggttccccaaaggggaacacctatacccaaccataggtaatttattacctatggcagtttctaaaacgtcgtaaatgattactcataaatcacaaaaagtgatttattcgcccctatggggccgctggcagagccaacgttcaaaaaacgttgttttttgtgcccgcacttaaggcttgtgtgctcattaaaacacacaagccctaatcttttgaatacaaagtgcggttaaaatttcaattattttttaatttta Mask Tandem Repeat Region ================================================ taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Find is-nt database================================================ Query_seq: AA00700:AA00702|AA00700:AA00702:iron(III) ABC transporter, ATP-binding protein:conserved hypothetical protein:->->:481519..481799 281 taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00700:AA00702|AA00700:AA00702:iron(III) ABC transporter, ATP-binding protein:conserved hypothetical protein:->->:481519..481799 281 taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00700:AA00702|AA00700:AA00702:iron(III) ABC transporter, ATP-binding protein:conserved hypothetical protein:->->:481519..481799 281 taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 59 End: 175 .......................................................... M C F N E H T S L K C G H K K Q R F L N V G S A S G P I G A N K S L F V I Y E .......................................................................................................... Protein_Len: 32 Strand: - Start: 87 End: 182 ...................................................................................... T R A C F V V N K S R Q S Q W R G W L P S Y I V K Q S K H T I M ................................................................................................... Protein_Len: 33 Strand: - Start: 53 End: 151 .................................................... P K H T K I L V C A K L A P V F F L T K Q V N A R G A A G Y P R M .................................................................................................................................. Protein_Len: 47 Strand: - Start: 28 End: 168 ........................... G C K T N L L N P S T H K L S C V L R L H P C L F C R K K F T P E A L P G M P A F L D S K T M ................................................................................................................. taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 55 HP_score: -3.0 Tail_Score: -4.18197 Start: 48 End: 89 Full_Region: gttttttgtgcccgc acttaaggcttgtgtgct cattaa aacacacaagccctaatc ttttgaatacaaagt ...............................................acttaaggcttgtgtgctcattaaaacacacaagccctaatc................................................................................................................................................................................................ TransTerm Strand: - Conf: 80 HP_score: -10.0 Tail_Score: -3.69216 Start: 50 End: 87 Full_Region: tttttgtgcccgcac ttaaggcttgtgtgct cattaa aacacacaagccctaa tcttttgaatacaaa .................................................ttaaggcttgtgtgctcattaaaacacacaagccctaa.................................................................................................................................................................................................. TransTerm Strand: + Conf: 95 HP_score: -13.7 Tail_Score: -3.05384 Start: 54 End: 83 Full_Region: TATTCAAAAGATTAG GGCTTGTGTGTTT TAAT GAGCACACAAGCC TTAAGTGCGGGCACA .....................................................GGCTTGTGTGTTTTAATGAGCACACAAGCC...................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -7.5 Tail_Score: -5.24129 Start: 104 End: 122 Full_Region: ggccgctggcagagc caacgtt caaaa aacgttg ttttttgtgcccgca .......................................................................................................caacgttcaaaaaacgttg............................................................................................................................................................... TransTerm Strand: - Conf: 72 HP_score: -7.9 Tail_Score: -3.87075 Start: 200 End: 229 Full_Region: gggaacacctatacc caaccataggtaa ttta ttacctatggcag tttctaaaacgtcgt .......................................................................................................................................................................................................caaccataggtaatttattacctatggcag.................................................... TransTerm Strand: + Conf: 91 HP_score: -12.6 Tail_Score: -3.14294 Start: 203 End: 226 Full_Region: ACGTTTTAGAAACTG CCATAGGTAA TAAA TTACCTATGG TTGGGTATAGGTGTT ..........................................................................................................................................................................................................CCATAGGTAATAAATTACCTATGG....................................................... Find igs database================================================ Query_seq: AA00700:AA00702|AA00700:AA00702:iron(III) ABC transporter, ATP-binding protein:conserved hypothetical protein:->->:481519..481799 281 taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Intra-Species Hit: Count: 1 Min: 1 Max: 281 Len: 281 Subject: aact_AA00700_AA00702|iron(III) ABC transporter, ATP-binding protein:conserved hypothetical protein|POSITIVE:POSITIVE|[481519,481799]|281 HSP 1 e-value: 1.0E-158 bit: 557.0 Len: 281 Query Start:1 Query End:281 Subject Strand: POSITIVE Subject Start: 1 Subject End: 281 taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac taaaattaaaaaataattgaaattttaaccgcactttgtattcaaaagattagggcttgtgtgttttaatgagcacacaagccttaagtgcgggcacaaaaaacaacgttttttgaacgttggctctgccagcggccccataggggcgaataaatcactttttgtgatttatgagtaatcatttacgacgttttagaaactgccataggtaataaattacctatggttgggtataggtgttcccctttggggaaccgataaaattcaaacttgaggacaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUAAAAAAUAAUUGAAAUUUUAACCGCACUUUGUAUUCAAAAGAUUAGGGCUUGUGUGUUUUAAUGAGCACACAAGCCUUAAGUGCGGGCACAAAAAACAACGUUUUUUGAACGUUGGCUCUGCCAGCGGCCCCAUAGGGGCGAAUAAAUCACUUUUUGUGAUUUAUGAGUAAUCAUUUACGACGUUUUAGAAACUGCCAUAGGUAAUAAAUUACCUAUGGUUGGGUAUAGGUGUUCCCCUUUGGGGAACCGAUAAAAUUCAAACUUGAGGACAAC ................(((((.(((((.((((((.(((....)))....(((((((((((((((((....)))))))))))))))))))))))...((((((((...))))))))..(((((((...))))))).(((((.(((((...(((((((((.....)))))))))((....))......((((..(...(..(.(((((((((((....))))))))))).)..)..)..))))))))).)))))......))))))))))............. (-100.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUGUCCUCAAGUUUGAAUUUUAUCGGUUCCCCAAAGGGGAACACCUAUACCCAACCAUAGGUAAUUUAUUACCUAUGGCAGUUUCUAAAACGUCGUAAAUGAUUACUCAUAAAUCACAAAAAGUGAUUUAUUCGCCCCUAUGGGGCCGCUGGCAGAGCCAACGUUCAAAAAACGUUGUUUUUUGUGCCCGCACUUAAGGCUUGUGUGCUCAUUAAAACACACAAGCCCUAAUCUUUUGAAUACAAAGUGCGGUUAAAAUUUCAAUUAUUUUUUAAUUUUA ..........((((.((((((((.(((..(((((.(((((........(((....((((((((((....))))))))))..(((.....)))...)))...........(((((((((.....)))))))))...))))).))))))))...((((((.(((((((.....)))))))..))))))..((((((((..((((((((((..........))))))))))....((....)).....)))))))).)))))))).)))).............. (-86.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 680 149 103 225 175 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 641 150 104 129 82 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 626 149 104 157 112 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 576 256 211 168 123 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 563 139 91 214 166 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 533 146 104 188 145 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 514 153 112 51 1 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 514 150 101 192 142 aact:AA01090|5end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_753548..753778_POSITIVE 460 250 202 164 116 aact:AA00978|5end_conserved_hypothetical_protein_683399..683629_POSITIVE 458 150 101 184 136 aact:AA02666|5end_DNA_helicase_II_1854410..1854640_POSITIVE 438 118 73 231 189 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 412 149 108 72 33 aact:AA01465|5end_mannose-specific_phosphotransferase_element_984472..984702_POSITIVE 395 149 115 167 116 aact:AA00413|5end_conserved_hypothetical_protein_280142..280372_POSITIVE 389 96 52 69 18 aact:AA01753|5end_conserved_hypothetical_protein_1185322..1185552_POSITIVE 375 130 81 229 174 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 374 147 104 229 177 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 367 257 217 70 22 aact:AA02599|5end_outer_membrane_protein,_Slp_family_1810083..1810313_POSITIVE 362 149 115 166 116 aact:AA02422|5end_conserved_hypothetical_protein_1668014..1668244_POSITIVE 362 149 115 166 116 aact:AA02298|5end_conserved_hypothetical_protein_1570588..1570818_POSITIVE 362 149 115 166 116 aact:AA00622|5end_conserved_hypothetical_protein_429692..429922_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 643 138 91 116 69 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 567 153 117 38 1 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 556 149 104 58 12 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 490 130 86 148 96 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 453 150 105 47 1 aact:AA01088|3end_rare_lipoprotein_B_precursor_753483..753633_POSITIVE 435 87 50 41 4 aact:AA00700|3end_iron(III)_ABC_transporter,_ATP-binding_protein_481458..481608_POSITIVE 427 155 116 50 11 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE 389 96 52 59 8 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 354 125 81 148 104 aact:AA02106|3end_hypothetical_protein_1433455..1433605_POSITIVE 346 148 118 131 105 aact:AA03010|3end_thiophene_and_furan_oxidation_protein_2102534..2102684_POSITIVE 332 147 108 113 68 aact:AA01299|3end_competence_protein_C_881502..881652_POSITIVE 331 150 104 144 100 aact:AA00723|3end_peptidase_B_(leucyl_aminopeptidase)_497841..497991_POSITIVE 327 148 101 151 97 aact:AA02762|3end_ribonuclease_H_1933708..1933858_POSITIVE 324 157 120 127 88 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 317 152 116 139 109 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 314 139 91 120 56 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 311 148 105 126 60 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 307 155 112 138 94 aact:AA02532|3end_conserved_hypothetical_protein_1762009..1762159_POSITIVE 307 149 104 131 74 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 304 60 28 98 67 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE