Origin IGS: aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcctcgacgatgtaaaagtgcggtggattttgccggcgtttttaggtcgtggagaggcattaaaaaacactgccaaaatccaccgcactttccgtgaaaaccaacaaaggagatctt Mask Tandem Repeat Region ================================================ aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Find is-nt database================================================ Query_seq: AA00675:AA00679|AA00675:AA00679:glutaredoxin 2:cytoplasmic axial filament (Ribonuclease G):->->:463309..463427 119 aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00675:AA00679|AA00675:AA00679:glutaredoxin 2:cytoplasmic axial filament (Ribonuclease G):->->:463309..463427 119 aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00675:AA00679|AA00675:AA00679:glutaredoxin 2:cytoplasmic axial filament (Ribonuclease G):->->:463309..463427 119 aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 4 End: 117 ... M S F V G F H G K C G G F W Q C F L M P L H D L K T P A K S T A L L H R R G .. Protein_Len: 30 Strand: + Start: 30 End: 119 ............................. M R W I L A V F F N A S P R P K N A G K I H R T F T S S R L Protein_Len: 33 Strand: - Start: 2 End: 100 . S R R Q Q N E R F T R H I K A T N K L A E G R G L F A P L I W R M ................... aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00675:AA00679|AA00675:AA00679:glutaredoxin 2:cytoplasmic axial filament (Ribonuclease G):->->:463309..463427 119 aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Intra-Species Hit: Count: 1 Min: 1 Max: 119 Len: 119 Subject: aact_AA00675_AA00679|glutaredoxin 2:cytoplasmic axial filament (Ribonuclease G)|POSITIVE:POSITIVE|[463309,463427]|119 HSP 1 e-value: 1.0E-61 bit: 236.0 Len: 119 Query Start:1 Query End:119 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta aagatctcctttgttggttttcacggaaagtgcggtggattttggcagtgttttttaatgcctctccacgacctaaaaacgccggcaaaatccaccgcacttttacatcgtcgaggcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAUCUCCUUUGUUGGUUUUCACGGAAAGUGCGGUGGAUUUUGGCAGUGUUUUUUAAUGCCUCUCCACGACCUAAAAACGCCGGCAAAAUCCACCGCACUUUUACAUCGUCGAGGCUA ..............(((((((.((((((((((((((((((((((.(.((((((((...................)))))))).).)))))))))))))))))))....))).))))))) (-46.01) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCUCGACGAUGUAAAAGUGCGGUGGAUUUUGCCGGCGUUUUUAGGUCGUGGAGAGGCAUUAAAAAACACUGCCAAAAUCCACCGCACUUUCCGUGAAAACCAACAAAGGAGAUCUU .....((..(..(((.((((((((((((((((((.(((.((((((..(((.......)))....)))))).))).))))))))))))))))))..((....))..)))..)..)).... (-43.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 445 104 61 205 158 aact:AA00296|5end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_202384..202614_POSITIVE 445 104 61 88 41 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 444 100 56 68 25 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 420 110 61 156 110 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 418 62 23 153 113 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 416 65 25 129 88 aact:AA01741|5end_6-pyruvoyl_tetrahydrobiopterin_synthase_1177193..1177423_POSITIVE 411 70 21 185 138 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 410 104 69 201 166 aact:AA02944|5end_30S_ribosomal_protein_S20_2058688..2058918_POSITIVE 410 104 69 48 13 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 409 61 25 147 110 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 408 69 21 153 104 aact:AA02860|5end_conserved_hypothetical_protein_2006074..2006304_POSITIVE 406 50 1 65 9 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 406 56 23 134 100 aact:AA00789|5end_DNA_topoisomerase_III_540486..540716_POSITIVE 405 70 21 112 57 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 403 68 23 157 113 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 401 100 51 128 68 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 401 100 51 62 2 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 393 104 74 177 147 aact:AA01564|5end_transaldolase_B_1049099..1049329_POSITIVE 393 70 26 151 106 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 390 70 25 228 182 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 445 104 61 79 32 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 444 100 56 87 44 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 422 110 79 98 63 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 420 110 61 151 105 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 418 62 23 46 6 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 416 65 25 99 58 aact:AA01740|3end_conserved_hypothetical_protein_1177243..1177393_POSITIVE 410 104 69 85 50 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 409 61 25 53 16 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 408 69 21 67 18 aact:AA02858|3end_conserved_hypothetical_protein_2006068..2006218_POSITIVE 406 50 1 74 18 aact:AA00865|3end_conserved_hypothetical_protein_600014..600164_POSITIVE 403 68 23 140 96 aact:AA02824|3end_conserved_hypothetical_protein_1986399..1986549_POSITIVE 393 104 74 41 11 aact:AA01563|3end_transketolase_isozyme_1_1049043..1049193_POSITIVE 390 70 25 54 8 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 387 62 21 85 44 aact:AA01019|3end_conserved_hypothetical_protein_712719..712869_POSITIVE 384 106 65 93 50 aact:AA02722|3end_cytidine_deaminase_1906352..1906502_POSITIVE 383 70 24 80 33 aact:AA01463|3end_probable_transport_protein_984200..984350_POSITIVE 380 98 52 65 7 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 379 55 15 57 13 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE 379 110 65 82 37 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 378 60 14 136 79 aact:AA00083|3end_hypothetical_protein_60851..61001_POSITIVE