Origin IGS: ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aggattgctttaggttgccattgattggagagttattgtaaatcagattatgccttttttaaatggtaatttcgtaaaattttgtcgcaaaaagggttttgttatcagaccggattagcgttaatatgcttgtcaacttatgtataaatcatcaaaataatttatttgctccgccagagttgttagctatgccaacgtttaaaaaatgtaaattttggtgcaccgcactttttctttgattttataggactctcc Mask Tandem Repeat Region ================================================ ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Find is-nt database================================================ Query_seq: AA00663:AA00664|AA00663:AA00664:cystathionine beta-lyase:phosphatidylglycerophosphate synthase (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase):->->:455995..456249 255 ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00663:AA00664|AA00663:AA00664:cystathionine beta-lyase:phosphatidylglycerophosphate synthase (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase):->->:455995..456249 255 ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00663:AA00664|AA00663:AA00664:cystathionine beta-lyase:phosphatidylglycerophosphate synthase (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase):->->:455995..456249 255 ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 141 End: 236 ............................................................................................................................................ D P R I V F G K K R C F K V F N G N L F P M I Q N V I V R W D M ................... Protein_Len: 46 Strand: - Start: 20 End: 157 ................... L F L A T C W F K C K K F T P M A L L E P P A F L N N Q H N I C L N V L M N V S I R D S L M .................................................................................................. ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 192 End: 221 ...............................................................................................................................................................................................ATTTAAAAAAGGCATAATCTGATTTACAAT.................................. PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 98 End: 127 .................................................................................................CTTGTCAACTTATGTATAAATCATCAAAAT................................................................................................................................ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 129 End: 158 ................................................................................................................................TTTGTTATCAGACCGGATTAGCGTTAATAT................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 93 HP_score: -6.8 Tail_Score: -5.43974 Start: 28 End: 38 Full_Region: aaaatgtaaattttg gtgc acc gcac tttttctttgatttt ...........................gtgcaccgcac......................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA00663:AA00664|AA00663:AA00664:cystathionine beta-lyase:phosphatidylglycerophosphate synthase (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase):->->:455995..456249 255 ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Intra-Species Hit: Count: 1 Min: 1 Max: 255 Len: 255 Subject: aact_AA00663_AA00664|cystathionine beta-lyase:phosphatidylglycerophosphate synthase (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase)|POSITIVE:POSITIVE|[455995, 456249]|255 HSP 1 e-value: 1.0E-142 bit: 505.0 Len: 255 Query Start:1 Query End:255 Subject Strand: POSITIVE Subject Start: 1 Subject End: 255 ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct ggagagtcctataaaatcaaagaaaaagtgcggtgcaccaaaatttacattttttaaacgttggcatagctaacaactctggcggagcaaataaattattttgatgatttatacataagttgacaagcatattaacgctaatccggtctgataacaaaaccctttttgcgacaaaattttacgaaattaccatttaaaaaaggcataatctgatttacaataactctccaatcaatggcaacctaaagcaatcct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAGAGUCCUAUAAAAUCAAAGAAAAAGUGCGGUGCACCAAAAUUUACAUUUUUUAAACGUUGGCAUAGCUAACAACUCUGGCGGAGCAAAUAAAUUAUUUUGAUGAUUUAUACAUAAGUUGACAAGCAUAUUAACGCUAAUCCGGUCUGAUAACAAAACCCUUUUUGCGACAAAAUUUUACGAAAUUACCAUUUAAAAAAGGCAUAAUCUGAUUUACAAUAACUCUCCAAUCAAUGGCAACCUAAAGCAAUCCU (((((((......((((((........((.(((...(((....................((((((...)))))).....(((((......(((((((((....)))))))))......(((....))).......)))))....))))))...)).....(((((((.......(((((....)))))........))))))).......))))))......)))))))........((........))...... (-43.86) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGAUUGCUUUAGGUUGCCAUUGAUUGGAGAGUUAUUGUAAAUCAGAUUAUGCCUUUUUUAAAUGGUAAUUUCGUAAAAUUUUGUCGCAAAAAGGGUUUUGUUAUCAGACCGGAUUAGCGUUAAUAUGCUUGUCAACUUAUGUAUAAAUCAUCAAAAUAAUUUAUUUGCUCCGCCAGAGUUGUUAGCUAUGCCAACGUUUAAAAAAUGUAAAUUUUGGUGCACCGCACUUUUUCUUUGAUUUUAUAGGACUCUCC .((...((.....))..)).......((((((((.....((((((((...(((.((((((((((((((.........(((((((.((((((...(((((.......))))).(((.(((((....))))).)))..............................)))))...).)))))))........)))))...))))))))).)))......(((((...))))).....)))))))).....)))))))) (-53.83) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 70 25 65 21 aact:AA01590|5end_hypothetical_protein_1070766..1070996_POSITIVE 331 70 25 107 63 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 308 70 21 128 62 aact:AA00587|5end_hypothetical_protein_399975..400205_POSITIVE 302 171 136 155 121 aact:AA02329|5end_conserved_hypothetical_protein_1589659..1589889_POSITIVE 294 70 21 167 115 aact:AA02666|5end_DNA_helicase_II_1854410..1854640_POSITIVE 291 60 12 178 132 aact:AA02380|5end_iron_chelatin_ABC_transporter,periplasmic-binding_protein_1628057..1628287_POSITIVE 291 60 12 75 29 aact:AA02379|5end_hypothetical_protein_1627954..1628184_POSITIVE 291 70 24 204 159 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 288 67 25 78 32 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 283 89 48 147 101 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 282 57 19 133 96 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 280 65 21 172 124 aact:AA02263|5end_conserved_hypothetical_protein_1539092..1539322_POSITIVE 274 100 58 95 48 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 270 70 22 160 99 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 264 70 24 170 125 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 261 87 57 204 161 aact:AA02605|5end_hypothetical_protein_1816555..1816785_POSITIVE 259 39 3 222 183 aact:AA01592|5end_hypothetical_protein_1070950..1071180_POSITIVE 259 70 30 90 51 aact:AA01177|5end_beta-ketoacyl-ACP_synthase_IV_806732..806962_POSITIVE 257 86 50 220 184 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 257 169 124 47 4 aact:AA00585|5end_glycerol-3-phosphate_regulon_repressor_399195..399425_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 70 25 90 46 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 330 65 23 102 61 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 308 70 21 99 33 aact:AA00586|3end_glycerol-3-phosphate_regulon_repressor_400026..400176_POSITIVE 287 70 21 75 21 aact:AA00135|3end_S-adenosylmethionine:_2-demethylmenaquinone_methyltransferase_(menaquinone_biosynthesis_protein)_96880..97030_POSITIVE 286 170 136 151 118 aact:AA02328|3end_anti-sigma_factor_B_antagonist_1589736..1589886_POSITIVE 283 89 48 95 49 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 274 100 58 92 45 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 261 87 57 151 108 aact:AA02604|3end_thioredoxin_reductase_1816582..1816732_POSITIVE 259 49 8 73 31 aact:AA01893|3end_acetyl-coenzyme-A_carboxylase_subunit_A_1277872..1278022_POSITIVE 256 87 59 75 47 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 256 173 137 69 36 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 253 65 24 147 107 aact:AA02106|3end_hypothetical_protein_1433455..1433605_POSITIVE 253 74 43 53 17 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 252 63 21 84 39 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 251 89 49 76 31 aact:AA02332|3end_conserved_hypothetical_protein;_possible_glycosyltransferase_1594392..1594542_POSITIVE 251 93 58 45 1 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 250 88 47 105 65 aact:AA02891|3end_oligopeptide-binding_protein_precursor_2026370..2026520_POSITIVE 245 90 58 55 19 aact:AA02684|3end_hydrogenase_4_membrane_subunit_1871612..1871762_POSITIVE 245 210 162 142 87 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 244 88 53 110 69 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE