Origin IGS: ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtctacctattcctaaaaggcttttggcgaaatccgaaagccttttttacagcctaaacttcaacaacattttcattctgtgctagaatgctccgatttttttatttccttttctattttccttctgaaggtcaaataatgaaaccctctattatcaataaattagatagcttaaaagaacgttatgaagagctggaagccttgttaggcgatgcttcc Mask Tandem Repeat Region ================================================ ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Find is-nt database================================================ Query_seq: AA00649:AA00650|AA00649:AA00650:peptide chain release factor 1 (RF-1):15 kDa peptidoglycan-associated protein:->->:444416..444637 222 ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00649:AA00650|AA00649:AA00650:peptide chain release factor 1 (RF-1):15 kDa peptidoglycan-associated protein:->->:444416..444637 222 ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00649:AA00650|AA00649:AA00650:peptide chain release factor 1 (RF-1):15 kDa peptidoglycan-associated protein:->->:444416..444637 222 ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 84 End: 188 ................................................................................... K V K L L F I S F S I F F D S C E L V S H F H Q Q L K P Q L F P K R M .................................. Protein_Len: 44 Strand: - Start: 1 End: 132 S A D G L L A E L E E Y R E K L S D L K N I I S P K M I Q G E S P F Y F L F Y F F R L M .......................................................................................... ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 70 HP_score: -6.7 Tail_Score: -4.11692 Start: 178 End: 201 Full_Region: TTAGGCTGTAAAAAA GGCTTTCGG ATTTCG CCAAAAGCC TTTTAGGAATAGGTA .................................................................................................................................................................................GGCTTTCGGATTTCGCCAAAAGCC..................... Find igs database================================================ Query_seq: AA00649:AA00650|AA00649:AA00650:peptide chain release factor 1 (RF-1):15 kDa peptidoglycan-associated protein:->->:444416..444637 222 ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Intra-Species Hit: Count: 1 Min: 1 Max: 222 Len: 222 Subject: aact_AA00649_AA00650|peptide chain release factor 1 (RF-1):15 kDa peptidoglycan-associated protein|POSITIVE:POSITIVE|[444416,444637]|222 HSP 1 e-value: 1.0E-110 bit: 398.0 Len: 222 Query Start:1 Query End:222 Subject Strand: POSITIVE Subject Start: 1 Subject End: 222 ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaatnnnnnnntcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta ggaagcatcgcctaacaaggcttccagctcttcataacgttcttttaagctatctaatttattgataatagagggtttcattatttgaccttcagaaggaaaatagaaaaggaaataaaaaaatcggagcattctagcacagaatgaaaatgttgttgaagtttaggctgtaaaaaaggctttcggatttcgccaaaagccttttaggaataggtagactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAAGCAUCGCCUAACAAGGCUUCCAGCUCUUCAUAACGUUCUUUUAAGCUAUCUAAUUUAUUGAUAAUAGAGGGUUUCAUUAUUUGACCUUCAGAAGGAAAAUAGAAAAGGAAAUAAAAAAAUCGGAGCAUUCUAGCACAGAAUGAAAAUGUUGUUGAAGUUUAGGCUGUAAAAAAGGCUUUCGGAUUUCGCCAAAAGCCUUUUAGGAAUAGGUAGACUUA .........(((((..........((((((((((((((((((((((....((((.........))))...(((((((.........))))))).)))))).............................((((((.....))))))...)))))).)))))....)))))...((((((((((.((......)).)))))))))).....)))))....... (-50.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUCUACCUAUUCCUAAAAGGCUUUUGGCGAAAUCCGAAAGCCUUUUUUACAGCCUAAACUUCAACAACAUUUUCAUUCUGUGCUAGAAUGCUCCGAUUUUUUUAUUUCCUUUUCUAUUUUCCUUCUGAAGGUCAAAUAAUGAAACCCUCUAUUAUCAAUAAAUUAGAUAGCUUAAAAGAACGUUAUGAAGAGCUGGAAGCCUUGUUAGGCGAUGCUUCC .((((............(((((((((((((......))))))))))))).....((((((.((((.......(((((((..(((.((.....(((....((((.(((((((((((..............)))))..)))))).))))...((((............)))).))).....)).)))..)))))))....)))).....))))))...)))).. (-43.74) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 218 176 147 103 aact:AA02844|5end_Holliday_junction_DNA_helicase_1996942..1997172_POSITIVE 288 50 6 183 125 aact:AA02404|5end_hypothetical_protein_1652378..1652608_POSITIVE 276 210 163 229 176 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 275 50 1 224 169 aact:AA01209|5end_conserved_hypothetical_protein_826430..826660_POSITIVE 275 50 1 82 27 aact:AA01207|5end_hypothetical_protein_826288..826518_POSITIVE 267 170 122 59 10 aact:AA02590|5end_conserved_hypothetical_protein_1801113..1801343_POSITIVE 267 170 121 64 9 aact:AA01629|5end_phosphatidylglycerophosphatase_A_1101881..1102111_POSITIVE 266 44 6 226 177 aact:AA02405|5end_conserved_hypothetical_protein_1652430..1652660_POSITIVE 265 194 151 192 134 aact:AA01357|5end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919133..919363_POSITIVE 265 220 189 224 186 aact:AA00684|5end_adenylosuccinate_synthase_467486..467716_POSITIVE 264 200 151 190 124 aact:AA02538|5end_conserved_hypothetical_protein_1765187..1765417_POSITIVE 264 218 180 197 168 aact:AA02315|5end_conserved_hypothetical_protein_1580482..1580712_POSITIVE 264 41 4 145 110 aact:AA00233|5end_hypothetical_protein_162339..162569_POSITIVE 263 194 153 155 108 aact:AA00120|5end_ATP-dependent_Clp_protease_ATP-binding_subunit_88963..89193_POSITIVE 262 219 179 81 32 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 262 205 169 191 157 aact:AA01369|5end_rod_shape-determining_protein_924948..925178_POSITIVE 262 207 166 182 139 aact:AA00519|5end_O-acetyltransferase_357139..357369_POSITIVE 261 219 176 209 169 aact:AA00049|5end_conserved_hypothetical_protein_39161..39391_POSITIVE 260 41 5 104 48 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 257 202 166 201 162 aact:AA02952|5end_lipoprotein_signal_peptidase_2065116..2065346_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 218 176 87 43 aact:AA02842|3end_conserved_hypothetical_protein_1996962..1997112_POSITIVE 276 210 163 135 82 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 266 41 3 151 110 aact:AA01113|3end_transcriptional_regulator_(merR_family)_765249..765399_POSITIVE 265 194 151 150 92 aact:AA01356|3end_lipooligosaccharide_galactosyltransferase_II_(comL_related)_919171..919321_POSITIVE 265 220 189 133 95 aact:AA00682|3end_S-adenosylmethionine-dependent_methyltransferase_467475..467625_POSITIVE 264 41 4 125 90 aact:AA00231|3end_long-chain-fatty-acid--CoA_ligase_162399..162549_POSITIVE 263 194 153 142 95 aact:AA00119|3end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_89030..89180_POSITIVE 262 205 169 75 41 aact:AA01368|3end_50S_ribosomal_protein_L19_924912..925062_POSITIVE 260 41 5 73 17 aact:AA01546|3end_deoxyribose_operon_repressor_1036320..1036470_POSITIVE 257 202 166 118 79 aact:AA02950|3end_isoleucyl-tRNA_synthetase_2065113..2065263_POSITIVE 253 214 179 83 35 aact:AA02956|3end_integrase-recombinase_2069454..2069604_POSITIVE 252 199 162 124 87 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE 252 201 166 144 109 aact:AA01519.1|3end_conserved_hypothetical_protein_1022920..1023070_POSITIVE 252 201 166 111 76 aact:AA01520|3end_hypothetical_protein_1022887..1023037_POSITIVE 252 41 1 74 42 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 251 216 179 91 43 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 250 186 141 128 83 aact:AA01801|3end_malate_dehydrogenase_1214370..1214520_POSITIVE 248 50 1 139 92 aact:AA01923|3end_type_III_restriction-modification_system_methylation_subunit_1299936..1300086_POSITIVE 248 195 154 121 72 aact:AA01624|3end_glucose-6-phosphate_isomerase_1099901..1100051_POSITIVE 247 170 126 47 5 aact:AA02589|3end_NAD(P)H_oxidoreductase_1801181..1801331_POSITIVE