Origin IGS: gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtttgattagattgaataaaaagtattctgcatcccaatcagataccacaaccaactaaactatggatttttatggtcaaacattattcaatcgaattcaaacatcatatcatccgccgacgtaagcaagcggaaaaaaacggtcatattgcgtcaaacttactcac Mask Tandem Repeat Region ================================================ gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Find is-nt database================================================ Query_seq: AA00643:AA00644|AA00643:AA00644:hypothetical protein:lipoprotein:->->:440755..440924 170 gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00643:AA00644|AA00643:AA00644:hypothetical protein:lipoprotein:->->:440755..440924 170 gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00643:AA00644|AA00643:AA00644:hypothetical protein:lipoprotein:->->:440755..440924 170 gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: - Start: 3 End: 152 .. L L N S A I H G N K E A Q K R R R I I H H K F E I S Y H K V M F I W L K T P Q P I Q N P H L I S K M .................. Protein_Len: 45 Strand: - Start: 2 End: 136 . S Y T Q R L I V T K K R K S V D A S S I I N S N S Q I I N S W L F G Y N L Q N H Y R I P M .................................. gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00643:AA00644|AA00643:AA00644:hypothetical protein:lipoprotein:->->:440755..440924 170 gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Intra-Species Hit: Count: 1 Min: 1 Max: 170 Len: 170 Subject: aact_AA00643_AA00644|hypothetical protein:lipoprotein|POSITIVE:POSITIVE|[440755, 440924]|170 HSP 1 e-value: 2.0E-79 bit: 295.0 Len: 170 Query Start:1 Query End:170 Subject Strand: POSITIVE Subject Start: 1 Subject End: 170 gtgagtaagtttgacgcaatatgaccgnnnnnnnccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta gtgagtaagtttgacgcaatatgaccgtttttttccgcttgcttacgtcggcggatgatatgatgtttgaattcgattgaataatgtttgaccataaaaatccatagtttagttggttgtggtatctgattgggatgcagaatactttttattcaatctaatcaaactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGAGUAAGUUUGACGCAAUAUGACCGUUUUUUUCCGCUUGCUUACGUCGGCGGAUGAUAUGAUGUUUGAAUUCGAUUGAAUAAUGUUUGACCAUAAAAAUCCAUAGUUUAGUUGGUUGUGGUAUCUGAUUGGGAUGCAGAAUACUUUUUAUUCAAUCUAAUCAAACUUA .....(((((((((...........((((.((.((((((.((....)).)))))).))...)))).........((((((((((.....(((((...((((.....))))...)))))...((((((.....))))))..........))))))))))...))))))))) (-40.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUUUGAUUAGAUUGAAUAAAAAGUAUUCUGCAUCCCAAUCAGAUACCACAACCAACUAAACUAUGGAUUUUUAUGGUCAAACAUUAUUCAAUCGAAUUCAAACAUCAUAUCAUCCGCCGACGUAAGCAAGCGGAAAAAAACGGUCAUAUUGCGUCAAACUUACUCAC ((((((((((..((((((((((...((((.(((.((....))))))))).............(((((((....)))))))......))))))))))...................(((((...(....)...)))))..................))))))))))..... (-34.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 442 53 12 165 114 aact:AA01152|5end_sec-independent_protein_translocase_protein_789364..789594_POSITIVE 435 100 54 209 163 aact:AA02630|5end_hypothetical_protein_1830296..1830526_POSITIVE 398 49 5 230 183 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 370 140 92 68 13 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 359 54 12 139 97 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 342 54 15 146 106 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 342 77 34 222 180 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 336 54 24 157 130 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 325 55 14 101 49 aact:AA02544|5end_ATP-dependent_RNA_helicase_1773317..1773547_POSITIVE 323 57 11 181 142 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 321 54 11 62 13 aact:AA01106|5end_conserved_hypothetical_protein_761675..761905_POSITIVE 319 79 40 48 18 aact:AA00055|5end_cytochrome_c-type_biogenesis_protein_41967..42197_POSITIVE 316 59 11 151 97 aact:AA02565|5end_transketolase,_N-terminal_subunit_1786366..1786596_POSITIVE 316 69 26 144 92 aact:AA01843|5end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1238377..1238607_POSITIVE 311 69 26 87 26 aact:AA02756|5end_phosphoserine_aminotransferase_1929477..1929707_POSITIVE 308 61 25 213 170 aact:AA01927|5end_conserved_hypothetical_protein_1300928..1301158_POSITIVE 306 75 35 86 35 aact:AA02724|5end_seryl-tRNA_ligase_1906357..1906587_POSITIVE 301 79 34 50 12 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 301 54 11 172 133 aact:AA01566|5end_conserved_hypothetical_protein_1051811..1052041_POSITIVE 301 54 16 35 1 aact:AA01496|5end_glucose_inhibited_division_protein_A_1005125..1005355_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 442 53 12 143 92 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 435 100 54 107 61 aact:AA02629|3end_hypothetical_protein_1830274..1830424_POSITIVE 380 93 54 150 112 aact:AA02419|3end_hypothetical_protein_1661954..1662104_POSITIVE 359 54 12 134 92 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 342 54 15 143 103 aact:AA01417|3end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_954028..954178_POSITIVE 330 102 61 145 104 aact:AA02681|3end_hypothetical_protein_1865951..1866101_POSITIVE 325 55 14 138 86 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 325 54 15 137 98 aact:AA00076|3end_dihydroneopterin_aldolase_56083..56233_POSITIVE 320 141 109 51 16 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 306 75 35 102 51 aact:AA02723|3end_hypothetical_protein_1906453..1906603_POSITIVE 306 68 21 53 2 aact:AA00729|3end_possible_hemolysin_(TlyC-like)_501177..501327_POSITIVE 301 57 11 64 21 aact:AA00333|3end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_226561..226711_POSITIVE 299 57 25 146 107 aact:AA01117|3end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_769375..769525_POSITIVE 295 75 46 66 29 aact:AA01098|3end_bis(5'-nucleosyl)-tetraphosphatase_758603..758753_POSITIVE 293 77 36 41 1 aact:AA00534|3end_hypothetical_protein_365594..365744_POSITIVE 288 60 14 147 96 aact:AA02821|3end_ribosomal-protein-alanine_acetyltransferase_1982775..1982925_POSITIVE 287 67 23 86 35 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 287 86 41 77 32 aact:AA01588|3end_N-acetylmuramoyl-L-alanine_amidase_II_1070380..1070530_POSITIVE 287 60 15 53 5 aact:AA01182|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_810510..810660_POSITIVE 286 59 15 89 36 aact:AA02735|3end_DNA_repair_protein_1917424..1917574_POSITIVE