Origin IGS: tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaacgccttacaatattgttctgtaagacacctatagtgtgtaacaaaaatttacaaaggcaagtatttttttgttaaaacttgcttaaaaatagcaaagtacaattaataaaaagtcacaataagatgaaatatatcgttttttagattttaattttgaagcaaaataaaatttacaaaaaatttaatataggtca Mask Tandem Repeat Region ================================================ tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Find is-nt database================================================ Query_seq: AA00637:AA00640|AA00637:AA00640:phosphohistidine phosphatase:hypothetical protein:->->:438735..438932 198 tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00637:AA00640|AA00637:AA00640:phosphohistidine phosphatase:hypothetical protein:->->:438735..438932 198 tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00637:AA00640|AA00637:AA00640:phosphohistidine phosphatase:hypothetical protein:->->:438735..438932 198 tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 14 End: 109 ............. M F C K F Y F A S K L K S K K R Y I S S Y C D F L L I V L C Y F ......................................................................................... tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00637:AA00640|AA00637:AA00640:phosphohistidine phosphatase:hypothetical protein:->->:438735..438932 198 tgacctatattaaattttttgtaaattttattttgcttcaaaattaaaatctaaaaaacgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Intra-Species Hit: Count: 1 Min: 59 Max: 198 Len: 140 Subject: aact_AA00637_AA00640|phosphohistidine phosphatase:hypothetical protein|POSITIVE:POSITIVE|[438735,438932]|198 HSP 1 e-value: 2.0E-61 bit: 236.0 Len: 140 Query Start:59 Query End:198 Subject Strand: POSITIVE Subject Start: 59 Subject End: 198 ..........................................................cgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacnnnnnnntacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta ..........................................................cgatatatttcatcttattgtgactttttattaattgtactttgctatttttaagcaagttttaacaaaaaaatacttgcctttgtaaatttttgttacacactataggtgtcttacagaacaatattgtaaggcgttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAUAUAUUUCAUCUUAUUGUGACUUUUUAUUAAUUGUACUUUGCUAUUUUUAAGCAAGUUUUAACAAAAAAAUACUUGCCUUUGUAAAUUUUUGUUACACACUAUAGGUGUCUUACAGAACAAUAUUGUAAGGCGUUUA .((......))..(((((.(((..................((((((.......))))))...(((((((((..(((........)))..)))))))))..))).))))).(((((((((.......)))))))))..... (-26.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACGCCUUACAAUAUUGUUCUGUAAGACACCUAUAGUGUGUAACAAAAAUUUACAAAGGCAAGUAUUUUUUUGUUAAAACUUGCUUAAAAAUAGCAAAGUACAAUUAAUAAAAAGUCACAAUAAGAUGAAAUAUAUCG .....(((((.....(((....(((.(.((((.....)))).).)))..))).....)))))..((..(((((((((((.(((((((.......))).))))....)))))))))))..)).....((((.....)))). (-24.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 243 123 83 53 7 aact:AA00938|5end_L-xylulokinase_647556..647786_POSITIVE 240 137 103 157 123 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 222 80 31 155 102 aact:AA01839|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_1236870..1237100_POSITIVE 218 137 101 168 127 aact:AA00079|5end_disulfide_bond_formation_protein_B_(disulfide_oxidoreductase)_57660..57890_POSITIVE 206 140 105 154 119 aact:AA00639|5end_conserved_hypothetical_protein_438821..439051_POSITIVE 203 115 78 203 161 aact:AA02172|5end_conserved_hypothetical_protein_1486019..1486249_POSITIVE 198 58 17 150 114 aact:AA01994|5end_chorismate_mutase;_prephenate_dehydrogenase_1354959..1355189_POSITIVE 198 49 6 210 156 aact:AA01462|5end_xylose_operon_regulatory_protein_981530..981760_POSITIVE 198 49 6 92 38 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 197 118 81 222 183 aact:AA01533|5end_conserved_hypothetical_protein_1028931..1029161_POSITIVE 197 118 81 62 23 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 193 109 77 220 184 aact:AA01325|5end_formamidopyrimidine-DNA_glycosylase_894747..894977_POSITIVE 192 119 79 145 100 aact:AA02677|5end_formate_dehydrogenase,_beta_subunit_1864102..1864332_POSITIVE 189 74 34 189 145 aact:AA02549|5end_iron_(chelated)_ABC_transporter,_periplasmic-binding_protein_1775870..1776100_POSITIVE 189 111 71 73 24 aact:AA01166|5end_sulphite_synthesis_pathway_protein_798599..798829_POSITIVE 184 126 81 65 6 aact:AA02814|5end_hypothetical_protein_1980180..1980410_POSITIVE 182 138 107 62 26 aact:AA02225|5end_ABC_transporter_ATP-binding_protein_1513202..1513432_POSITIVE 177 120 77 205 160 aact:AA00788|5end_hypothetical_protein_540351..540581_POSITIVE 175 136 94 221 160 aact:AA01873|5end_mannose-6-phosphate_isomerase_1260658..1260888_POSITIVE 175 138 91 206 164 aact:AA00021|5end_DNA_helicase_IV_14630..14860_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 222 80 31 148 95 aact:AA01838|3end_conserved_hypothetical_protein_1236943..1237093_POSITIVE 218 137 101 119 78 aact:AA00078|3end_hypothetical_protein_57691..57841_POSITIVE 201 120 79 75 30 aact:AA02421|3end_conserved_hypothetical_protein_1667300..1667450_POSITIVE 201 120 79 104 59 aact:AA02297|3end_conserved_hypothetical_protein_1569900..1570050_POSITIVE 201 120 79 104 59 aact:AA00621|3end_conserved_hypothetical_protein_429007..429157_POSITIVE 201 120 79 104 59 aact:AA00412|3end_conserved_hypothetical_protein_279454..279604_POSITIVE 198 58 17 115 79 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 198 49 6 92 38 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 197 118 81 88 49 aact:AA01531|3end_hypothetical_protein_1028877..1029027_POSITIVE 193 109 77 135 99 aact:AA01324|3end_50S_ribosomal_protein_L33_894742..894892_POSITIVE 192 119 79 140 95 aact:AA02676|3end_formate_dehydrogenase_alpha_subunit_1864177..1864327_POSITIVE 189 74 34 88 44 aact:AA02548|3end_desulfoviridin_gamma_subunit_1775849..1775999_POSITIVE 184 126 81 103 44 aact:AA02813|3end_hypothetical_protein_1980298..1980448_POSITIVE 182 138 107 62 26 aact:AA02224|3end_ABC_transport_system_permease_protein_1513282..1513432_POSITIVE 179 90 53 51 16 aact:AA01551|3end_hypothetical_protein_1038374..1038524_POSITIVE 175 136 94 127 66 aact:AA01872|3end_conserved_hypothetical_protein_1260644..1260794_POSITIVE 174 138 105 104 78 aact:AA01637|3end_ribonucleoside_diphosphate_reductase_1109004..1109154_POSITIVE 171 140 93 62 8 aact:AA02319|3end_nitrogen_regulatory_IIA_protein_1584505..1584655_POSITIVE 170 119 103 55 37 aact:AA00570|3end_hypothetical_protein_391785..391935_POSITIVE 168 81 41 123 82 aact:AA02024|3end_acyl_carrier_protein_phosphodiesterase_1378739..1378889_POSITIVE