Origin IGS: taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgggtattttaggtttagtataaggtgattttacgccatcaagtcctctttcacgaaaggcttttaatcaataaatgacttgagaatgaggaatctgatgaagtttagccacctcacggataccaaatcctgttcggtgacctgtttgataacttttagatgaaattgataagagtagggcataatctgcacttcaaattagatgcccgggtttgggggcagattaaagtgcggtcagtttgaaaaaagttttttaaaccaaccgcactttttaggcgttgaatta Mask Tandem Repeat Region ================================================ taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Find is-nt database================================================ Query_seq: AA00629:AA00631|AA00629:AA00631:inosine 5' monophosphate dehydrogenase (IMP dehydrogenase):hypothetical protein:->->:433530..433817 288 taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00629:AA00631|AA00629:AA00631:inosine 5' monophosphate dehydrogenase (IMP dehydrogenase):hypothetical protein:->->:433530..433817 288 taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00629:AA00631|AA00629:AA00631:inosine 5' monophosphate dehydrogenase (IMP dehydrogenase):hypothetical protein:->->:433530..433817 288 taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 3 End: 128 .. M Q R L K S A V G L K N F F Q T D R T L I C P Q T R A S N L K C R L C P T L I N F I ................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 121 End: 216 ........................................................................................................................ M S S K S Y Q T G H R T G F G I R E V A K L H Q I P H S Q V I Y ........................................................................ Protein_Len: 42 Strand: + Start: 95 End: 220 .............................................................................................. M Q I M P Y S Y Q F H L K V I K Q V T E Q D L V S V R W L N F I R F L I L K S F I D .................................................................... Protein_Len: 30 Strand: - Start: 127 End: 216 .............................................................................................................................. R F T I L C T V S C S K T D T L H S F K M L N R M R L D N M ........................................................................ taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 93 HP_score: -11.6 Tail_Score: -4.62471 Start: 19 End: 58 Full_Region: tgggggcagattaaa gtgcggtcagtttgaaaa aagt tttttaaaccaaccgcac tttttaggcgttgaa ..................gtgcggtcagtttgaaaaaagttttttaaaccaaccgcac...................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA00629:AA00631|AA00629:AA00631:inosine 5' monophosphate dehydrogenase (IMP dehydrogenase):hypothetical protein:->->:433530..433817 288 taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Intra-Species Hit: Count: 4 Min: 1 Max: 288 Len: 288 Subject: aact_AA00629_AA00631|inosine 5' monophosphate dehydrogenase (IMP dehydrogenase):hypothetical protein|POSITIVE:POSITIVE|[433530,433817]|288 HSP 1 e-value: 1.0E-162 bit: 571.0 Len: 288 Query Start:1 Query End:288 Subject Strand: POSITIVE Subject Start: 1 Subject End: 288 taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa taattcaacgcctaaaaagtgcggttggtttaaaaaacttttttcaaactgaccgcactttaatctgcccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggatttggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaatacccaaa Subject: aact_AA01402_AA01404|beta 1-4 glucosyltransferase:hypothetical protein|POSITIVE:POSITIVE|[948443,948717]|275 HSP 1 e-value: 2.0E-56 bit: 220.0 Len: 213 Query Start:68 Query End:280 Subject Strand: POSITIVE Subject Start: 23 Subject End: 236 ...................................................................cccccaaacccgggcatctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggattt-ggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattg-attaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaa........ ...................................................................ccccaaaacctggacacctaatttgaggtgcagattatgtcttactcttatcaatttcgtttagaaattatcaaacaggtcacctagcaggattttggtatccgtgaggtggctgaacttcatcagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacac-aaacctaaaa........ Subject: aact_AA01547_AA01549|deoxyribose-phosphate aldolase:hypothetical protein|POSITIVE:POSITIVE|[1037080,1037583]|504 HSP 1 e-value: 7.0E-44 bit: 178.0 Len: 196 Query Start:85 Query End:280 Subject Strand: POSITIVE Subject Start: 173 Subject End: 366 ....................................................................................ctaatttgaagtgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggattt-ggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaa........ ....................................................................................ctaatttgaggtgcagattatgtcttactcttaccaatttcgtttaaaaattatcaagccggtcaccgagcaggatttcggtatatgagcggtggctaaatttcatcagatttctcgttctcaagtcatttattgg--aaaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatac-aaacctaaaa........ Subject: aact_AA00227_AA00229|hypothetical sequence:DNA polymerase III, alpha subunit|POSITIVE:POSITIVE|[156703,157002]|300 HSP 1 e-value: 6.0E-26 bit: 119.0 Len: 185 Query Start:96 Query End:280 Subject Strand: NEGATIVE Subject Start: 40 Subject End: 212 ...............................................................................................tgcagattatgccctactcttatcaatttcatctaaaagttatcaaacaggtcaccgaacaggattt-ggtatccgtgaggtggctaaacttcatcagattcctcattctcaagtcatttattgattaaaagcctttcgtgaaagaggacttgatggcgtaaaatcaccttatactaaacctaaaa........ ...............................................................................................tgcagattatgtcctactcttatcaatttcgtttaaaaattatcaagctagtcaccgagcaggatttcggtatatgagaggtggctaa----------atttctcgttctcaagtcatttattgg--aaaagcctttcgtgaaagagggcttgatggcgtaaaatcaccttacac-aaacctaaaa........ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCAACGCCUAAAAAGUGCGGUUGGUUUAAAAAACUUUUUUCAAACUGACCGCACUUUAAUCUGCCCCCAAACCCGGGCAUCUAAUUUGAAGUGCAGAUUAUGCCCUACUCUUAUCAAUUUCAUCUAAAAGUUAUCAAACAGGUCACCGAACAGGAUUUGGUAUCCGUGAGGUGGCUAAACUUCAUCAGAUUCCUCAUUCUCAAGUCAUUUAUUGAUUAAAAGCCUUUCGUGAAAGAGGACUUGAUGGCGUAAAAUCACCUUAUACUAAACCUAAAAUACCCAAA .........((((..((((((((((..((((.((((....)))).))))..)))))))))).................(((((..(((((((.....))))))))))))..................................))))........(((.((((((((....((((((..(..((.(((((((..(((((.(((.((((.(.((((.....)))).).)))..).))).))))).))))))).))..)..)))))))))))))))))............ (-74.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGGGUAUUUUAGGUUUAGUAUAAGGUGAUUUUACGCCAUCAAGUCCUCUUUCACGAAAGGCUUUUAAUCAAUAAAUGACUUGAGAAUGAGGAAUCUGAUGAAGUUUAGCCACCUCACGGAUACCAAAUCCUGUUCGGUGACCUGUUUGAUAACUUUUAGAUGAAAUUGAUAAGAGUAGGGCAUAAUCUGCACUUCAAAUUAGAUGCCCGGGUUUGGGGGCAGAUUAAAGUGCGGUCAGUUUGAAAAAAGUUUUUUAAACCAACCGCACUUUUUAGGCGUUGAAUUA ...(((.((((..(((...((...(((((..(..((..(((((..(((((.(((.(....)........((((........))))))).)))))...)))))..))..)..))))).))....))))))))))((((..((.((((.......(((((((...........)))))))......((((((((.((((((((..(.....)..))))))))))))))))(((((((((..(((((((((....)))))))))..)))))))))..))))))..)))).. (-80.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 787 190 142 50 1 aact:AA01445|5end_hypothetical_protein_969899..970129_POSITIVE 702 106 63 217 172 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 683 100 51 162 111 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 512 70 21 168 118 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 510 60 12 149 102 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 506 60 15 219 174 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 500 96 51 155 105 aact:AA00536|5end_5'-nucleotidase_precursor_366151..366381_POSITIVE 499 140 93 224 176 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 496 60 15 168 123 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 496 60 15 56 11 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 488 100 51 160 101 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 468 60 16 162 118 aact:AA01967|5end_hypothetical_protein_1331511..1331741_POSITIVE 463 60 11 74 29 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 452 60 14 150 104 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 446 70 21 177 125 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 420 60 15 158 113 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 411 83 41 179 140 aact:AA00632|5end_inner_membrane_protein_ImpA_434127..434357_POSITIVE 401 83 51 177 143 aact:AA01407|5end_outer_membrane_antigenic_lipoprotein_b_precursor_949005..949235_POSITIVE 400 60 14 170 124 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 395 60 19 152 116 aact:AA00300|5end_conserved_hypothetical_protein_203785..204015_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 717 280 231 51 3 aact:AA00227|3end_hypothetical_sequence_156642..156792_POSITIVE 702 106 63 93 48 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 683 100 51 101 50 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 510 60 12 75 28 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 506 60 15 81 36 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 496 60 15 72 27 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 474 60 15 74 29 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 468 60 16 85 41 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 452 60 14 88 42 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 426 83 50 81 46 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 413 69 21 151 100 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 410 60 16 74 30 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 390 60 12 84 34 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 388 60 15 73 20 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 375 98 51 57 22 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 368 97 51 78 24 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 360 67 21 44 1 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 360 63 21 44 2 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 358 78 46 105 64 aact:AA02190|3end_hydrogenase-2_component_protein_1493271..1493421_POSITIVE 351 100 52 127 78 aact:AA00131|3end_transcriptional_regulator_(MerR_family)_94984..95134_POSITIVE