Origin IGS: taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaattagtgacttgtctagtaaataagtaaacgcaggcgataaagataaaataataagcggcagtatttttgtttatgctgacttaaacgactttttctcattgggtattctaacctgaatggaattttgtttcgttatctagtacagtccttaaacctaagttaaaattaggttcttttgaatttatgaacaaaaagtgcatttttttgccaacatcacccgattcaaaagtatgacacacaaaatcatcttcagcgctataagtgcccgcactgcaataaagtgttttctttcaaaagtaaacttaatccaatacaaatttgggaagattataaaaattactctttggtaagagcctgcaatgttatcttgataaagcgcctaaaaccacgctaaattcctctgaatatccttactggcattactttttttgttgggatttttacgtgcttgcgcttttaaaactagtgtttattaaatattttctgaataaaaagaaccagtaaaaacttgtttggctgttctgttaaaacaactaaagagcattatatttttaacggtgctta Mask Tandem Repeat Region ================================================ taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Find is-nt database================================================ Query_seq: AA00618:AA00619|AA00618:AA00619:molybdopterin biosynthesis protein:hypothetical protein:->->:423703..424270 568 taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00618:AA00619|AA00618:AA00619:molybdopterin biosynthesis protein:hypothetical protein:->->:423703..424270 568 taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00618:AA00619|AA00618:AA00619:molybdopterin biosynthesis protein:hypothetical protein:->->:423703..424270 568 taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: + Start: 204 End: 380 ........................................................................................................................................................................................................... M A G S Y Q R V I F I I F P N L Y W I K F T F E R K H F I A V R A L I A L K M I L C V I L L N R V M L A K K C T F C S ............................................................................................................................................................................................ Protein_Len: 43 Strand: + Start: 326 End: 454 ..................................................................................................................................................................................................................................................................................................................................... M C H T F E S G D V G K K M H F L F I N S K E P N F N L G L R T V L D N E T K F H S G .................................................................................................................. Protein_Len: 36 Strand: + Start: 442 End: 549 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................... M P F R L E Y P M R K S R L S Q H K Q K Y C R L L F Y L Y R L R L L I Y ................... Protein_Len: 56 Strand: - Start: 218 End: 385 ......................................................................................................................................................................................................................... W L T I K I I K G F K Y Q I L N V K S L F C K I A T R A S I A S F I I K H T M S K F R T I N A F F H V K Q E Y M ....................................................................................................................................................................................... Protein_Len: 57 Strand: - Start: 174 End: 344 ............................................................................................................................................................................. R P K L R K I L I V N C A R V L S Y N K Y D E W I Q I P N L K S K F S F V K N C H P C K Y R Q L H N Q T D Y K Q M ................................................................................................................................................................................................................................ Protein_Len: 49 Strand: - Start: 64 End: 210 ............................................................... Q N K K N L F Y K I F V L K L L A L V Y F D W C F F Y H W Y P Y E S S N L T T K P A K D L Y C Q M ...................................................................................................................................................................................................................................................................................................................................................................... taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00618:AA00619|AA00618:AA00619:molybdopterin biosynthesis protein:hypothetical protein:->->:423703..424270 568 taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Intra-Species Hit: Count: 1 Min: 1 Max: 568 Len: 568 Subject: aact_AA00618_AA00619|molybdopterin biosynthesis protein:hypothetical protein|POSITIVE:POSITIVE|[423703,424270]|568 HSP 1 e-value: 0.0 bit: 1043.0 Len: 568 Query Start:1 Query End:568 Subject Strand: POSITIVE Subject Start: 1 Subject End: 568 taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacnnnnnnngtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcnnnnnnntgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta taagcaccgttaaaaatataatgctctttagttgttttaacagaacagccaaacaagtttttactggttctttttattcagaaaatatttaataaacactagttttaaaagcgcaagcacgtaaaaatcccaacaaaaaaagtaatgccagtaaggatattcagaggaatttagcgtggttttaggcgctttatcaagataacattgcaggctcttaccaaagagtaatttttataatcttcccaaatttgtattggattaagtttacttttgaaagaaaacactttattgcagtgcgggcacttatagcgctgaagatgattttgtgtgtcatacttttgaatcgggtgatgttggcaaaaaaatgcactttttgttcataaattcaaaagaacctaattttaacttaggtttaaggactgtactagataacgaaacaaaattccattcaggttagaatacccaatgagaaaaagtcgtttaagtcagcataaacaaaaatactgccgcttattattttatctttatcgcctgcgtttacttatttactagacaagtcactaattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCACCGUUAAAAAUAUAAUGCUCUUUAGUUGUUUUAACAGAACAGCCAAACAAGUUUUUACUGGUUCUUUUUAUUCAGAAAAUAUUUAAUAAACACUAGUUUUAAAAGCGCAAGCACGUAAAAAUCCCAACAAAAAAAGUAAUGCCAGUAAGGAUAUUCAGAGGAAUUUAGCGUGGUUUUAGGCGCUUUAUCAAGAUAACAUUGCAGGCUCUUACCAAAGAGUAAUUUUUAUAAUCUUCCCAAAUUUGUAUUGGAUUAAGUUUACUUUUGAAAGAAAACACUUUAUUGCAGUGCGGGCACUUAUAGCGCUGAAGAUGAUUUUGUGUGUCAUACUUUUGAAUCGGGUGAUGUUGGCAAAAAAAUGCACUUUUUGUUCAUAAAUUCAAAAGAACCUAAUUUUAACUUAGGUUUAAGGACUGUACUAGAUAACGAAACAAAAUUCCAUUCAGGUUAGAAUACCCAAUGAGAAAAAGUCGUUUAAGUCAGCAUAAACAAAAAUACUGCCGCUUAUUAUUUUAUCUUUAUCGCCUGCGUUUACUUAUUUACUAGACAAGUCACUAAUUUA .((((.((....(((((...(((((((((.(((((((.....))))))).......((((((((((((.((((((..................((((....)))).....((....))..................))))))....)))))))))))).....))))).....)))).))))).)).))))....(((((..........((((((....))))))..........))))).....((((((.((((..(((((..((.((..((((...((((.(((((.((((((...........))))))..)))))....))))......))))..)).(((((((((..(..((((.((((((.((((((((.............((((((((.......))))))))...(.(((.(((....(((((.......(((((((..(((......))).)))).)))....)))))..)))))))...))))))))...))).....))).))))..)..))))))))).))..)))))....)))))))))).......... (-115.65) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUAGUGACUUGUCUAGUAAAUAAGUAAACGCAGGCGAUAAAGAUAAAAUAAUAAGCGGCAGUAUUUUUGUUUAUGCUGACUUAAACGACUUUUUCUCAUUGGGUAUUCUAACCUGAAUGGAAUUUUGUUUCGUUAUCUAGUACAGUCCUUAAACCUAAGUUAAAAUUAGGUUCUUUUGAAUUUAUGAACAAAAAGUGCAUUUUUUUGCCAACAUCACCCGAUUCAAAAGUAUGACACACAAAAUCAUCUUCAGCGCUAUAAGUGCCCGCACUGCAAUAAAGUGUUUUCUUUCAAAAGUAAACUUAAUCCAAUACAAAUUUGGGAAGAUUAUAAAAAUUACUCUUUGGUAAGAGCCUGCAAUGUUAUCUUGAUAAAGCGCCUAAAACCACGCUAAAUUCCUCUGAAUAUCCUUACUGGCAUUACUUUUUUUGUUGGGAUUUUUACGUGCUUGCGCUUUUAAAACUAGUGUUUAUUAAAUAUUUUCUGAAUAAAAAGAACCAGUAAAAACUUGUUUGGCUGUUCUGUUAAAACAACUAAAGAGCAUUAUAUUUUUAACGGUGCUUA ..........((((((.......))))))....((((((......................(((...((((((((((((((((((..(((((..(.(((.((((((((......)))).)))))))....)..)))))....))).)))).....(((((((.......)))))))...........))))))))))).)))....(((((((((..............((((...(((((...............((((.....))))..((...))......)))))..)))).....((((.(((..(((((.......)))))))).))))............)))))))))))))))...............(((((((.....(((.((...((((....))))....(((((((......((((((((((.(((.............(((((.........))))).............))).)))))))))).)))))))......)).))).((((((..............)))))).............))))))). (-112.31) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 354 190 141 77 24 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 328 338 291 173 135 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 312 190 148 83 52 aact:AA02873|5end_conserved_hypothetical_protein_2016165..2016395_POSITIVE 310 328 281 147 92 aact:AA01611|5end_anthranilate_synthase_component_II_1088976..1089206_POSITIVE 308 334 291 187 147 aact:AA00534|5end_hypothetical_protein_365419..365649_POSITIVE 308 334 291 91 51 aact:AA00533|5end_conserved_hypothetical_protein_365323..365553_POSITIVE 307 330 282 190 138 aact:AA01248|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849734..849964_POSITIVE 307 330 282 120 68 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 302 328 284 155 114 aact:AA02827|5end_conserved_hypothetical_protein_1987021..1987251_POSITIVE 302 190 148 170 127 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 301 357 311 170 121 aact:AA02371|5end_oxygen-independent_coproporphyrinogen_III_oxidase_1624580..1624810_POSITIVE 301 220 174 156 106 aact:AA00545|5end_glutathionylspermidine_synthetase;amidase_373607..373837_POSITIVE 299 339 294 222 167 aact:AA00881|5end_hypothetical_protein_613172..613402_POSITIVE 297 227 182 90 51 aact:AA01128|5end_hypothetical_protein_774030..774260_POSITIVE 296 350 306 72 22 aact:AA00288|5end_conserved_hypothetical_protein_200485..200715_POSITIVE 294 329 282 156 119 aact:AA01451|5end_D-xylulokinase_971935..972165_POSITIVE 294 329 282 81 44 aact:AA01450|5end_hypothetical_protein_971860..972090_POSITIVE 291 328 284 71 10 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 287 218 171 111 58 aact:AA02271|5end_hypothetical_protein_1548623..1548853_POSITIVE 286 333 291 228 181 aact:AA02869|5end_hypothetical_protein_2013788..2014018_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 310 328 281 71 16 aact:AA01610|3end_hypothetical_protein_1088980..1089130_POSITIVE 308 316 291 138 116 aact:AA00674|3end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_462585..462735_POSITIVE 308 334 291 73 33 aact:AA00531|3end_DNA_polymerase_I_365385..365535_POSITIVE 307 330 282 57 5 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE 287 218 171 76 23 aact:AA02270|3end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1548668..1548818_POSITIVE 286 330 288 56 7 aact:AA00835|3end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_576353..576503_POSITIVE 281 322 284 66 24 aact:AA02235|3end_stringent_starvation_protein_B_1521984..1522134_POSITIVE 277 190 146 65 21 aact:AA02912|3end_hypothetical_protein_2039590..2039740_POSITIVE 272 360 311 112 68 aact:AA00733|3end_hypothetical_protein_502513..502663_POSITIVE 271 208 161 141 76 aact:AA02802|3end_probable_myo-inositol_2-dehydrogenase_1970086..1970236_POSITIVE 270 212 173 101 51 aact:AA01311|3end_transcriptional_regulator_889644..889794_POSITIVE 269 328 283 44 3 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 266 550 506 137 88 aact:AA02426|3end_uridylyltransferase_1674226..1674376_POSITIVE 265 159 112 85 25 aact:AA00692|3end_conserved_hypothetical_protein_474515..474665_POSITIVE 264 190 141 132 65 aact:AA02989|3end_conserved_hypothetical_protein_2089548..2089698_POSITIVE 261 321 289 131 92 aact:AA00927|3end_ATP-dependent_DNA_helicase_637777..637927_POSITIVE 260 214 174 103 60 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 259 329 283 130 71 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE 259 350 303 150 106 aact:AA00529|3end_outer_membrane_protein,_Omp_18/16_362370..362520_POSITIVE 258 332 291 111 56 aact:AA02350|3end_possible_lipoprotein_1609179..1609329_POSITIVE