Origin IGS: tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccaactttttctatttataagatccctaataagtaatacattgcgcatacttgtcaaactatgatgcacggctaatttttgttttaatattttaatcccttaagtcgattagttgtttttcaatgcctaaaccggcatattgttcaaacgtgaataactaattcatatatcgcttttgagctcctatacgcacttctgacattgcgctgtttataagaaaaattctcattcggcttgtccgagcgttccgtcaaaaacgtttgatgatttcgataccacgaatagcgcaggacataaaagtgatttttttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagattttcgcgctacttcatcacagaatccgatcgatactcctaaagtgcggtagtattttatggtgttttttaaaacccttgaaaagggtatagcagaaaagtagcggataaaagtgggttaagaccttatagtacgaggcgttaacccacttttttgaatttatcaacaaaaaaatcacttttatgtcctgcgctattcgtggtatcgaaatcatcaaacgtttttgacggaacgctcggacaagccgaatgagaatttttcttataaacagcgcaatgtcagaagtgcgtataggagctcaaaagcgatatatgaattagttattcacgtttgaacaatatgccggtttaggcattgaaaaacaactaatcgacttaagggattaaaatattaaaacaaaaattagccgtgcatcatagtttgacaagtatgcgcaatgtattacttattagggatcttataaatagaaaaagttggtaaaaccaactcttttgtctactatgcctgtatatttattaaacaataataaatatacaggtcctgttagtactcgatcgatgtctctaggtgttctatcagttaaattatca Mask Tandem Repeat Region ================================================ tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccaactttttctatttataagatccctaataagtaatacattgcgcatacttgtcaaactatgatgcacggctaatttttgttttaatattttaatcccttaagtcgattagttgtttttcaatgcctaaaccggcatattgttcaaacgtgaataactaattcatatatcgcttttgagctcctatacgcacttctgacattgcgctgtttataagaaaaattctcattcggcttgtccgagcgttccgtcaaaaacgtttgatgatttcgataccacgaatagcgcaggacataaaagtgatttttttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Find is-nt database================================================ Query_seq: AA00590:AA00591|AA00590:AA00591:outer membrane substrate binding protein; TonB-dependent outer membrane receptor:ABC transporter, ATP-binding protein:->->:403158..403752 595 tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccaactttttctatttataagatccctaataagtaatacattgcgcatacttgtcaaactatgatgcacggctaatttttgttttaatattttaatcccttaagtcgattagttgtttttcaatgcctaaaccggcatattgttcaaacgtgaataactaattcatatatcgcttttgagctcctatacgcacttctgacattgcgctgtttataagaaaaattctcattcggcttgtccgagcgttccgtcaaaaacgtttgatgatttcgataccacgaatagcgcaggacataaaagtgatttttttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00590:AA00591|AA00590:AA00591:outer membrane substrate binding protein; TonB-dependent outer membrane receptor:ABC transporter, ATP-binding protein:->->:403158..403752 595 tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccaactttttctatttataagatccctaataagtaatacattgcgcatacttgtcaaactatgatgcacggctaatttttgttttaatattttaatcccttaagtcgattagttgtttttcaatgcctaaaccggcatattgttcaaacgtgaataactaattcatatatcgcttttgagctcctatacgcacttctgacattgcgctgtttataagaaaaattctcattcggcttgtccgagcgttccgtcaaaaacgtttgatgatttcgataccacgaatagcgcaggacataaaagtgatttttttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00590:AA00591|AA00590:AA00591:outer membrane substrate binding protein; TonB-dependent outer membrane receptor:ABC transporter, ATP-binding protein:->->:403158..403752 595 tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccaactttttctatttataagatccctaataagtaatacattgcgcatacttgtcaaactatgatgcacggctaatttttgttttaatattttaatcccttaagtcgattagttgtttttcaatgcctaaaccggcatattgttcaaacgtgaataactaattcatatatcgcttttgagctcctatacgcacttctgacattgcgctgtttataagaaaaattctcattcggcttgtccgagcgttccgtcaaaaacgtttgatgatttcgataccacgaatagcgcaggacataaaagtgatttttttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 117 Max: 421 Len: 305 Subject: UniRef90_Q65Q87 Cluster: Hypothetical protein; n=2; Mannheimia succiniciproducens MBEL55E|Rep: Hypothetical protein - Mannheimia succiniciproducens (strain MBEL55E) HSP 1 e-value: 1.0E-10 bit: 57.0 Len: 216 Query Start:206 Query End:421 Subject Strand: null Subject Start: 129 Subject End: 202 ............................................................................................................................................................................................................. K N H F Y V L R Y S W Y R N H Q T F L T E R S D K P N E - - N F S Y K Q R N V R S A Y R S S K A I Y E L V I H V * T I C R F R H * K T T N R L K G L .............................................................................................................................................................................. ............................................................................................................................................................................................................. K N K F Y I N L H H W Y L K H K N F L N E R S S I P D K A G K Y P F K H R N L R S A Y S S L K R H E E F L F T F E K Y P E L K I E K T T N R L E G L .............................................................................................................................................................................. HSP 2 e-value: 1.0E-10 bit: 32.0 Len: 84 Query Start:117 Query End:200 Subject Strand: null Subject Start: 206 Subject End: 233 .................................................................................................................... L K Q K L A V H H S L T S M R N V L L I R D L I N R K S ........................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................................... L K R K L A L H N G L S K K N K I M F I K D F L N E K S ........................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_A3N0D2 Cluster: Hypothetical protein; n=2; Actinobacillus pleuropneumoniae L20|Rep: Hypothetical protein - Actinobacillus pleuropneumoniae serotype 5b (strain L20) HSP 1 e-value: 2.0E-9 bit: 64.7 Len: 216 Query Start:206 Query End:421 Subject Strand: null Subject Start: 95 Subject End: 168 ............................................................................................................................................................................................................. K N H F Y V L R Y S W Y R N H Q T F L T E R S D K P N E N - - F S Y K Q R N V R S A Y R S S K A I Y E L V I H V * T I C R F R H * K T T N R L K G L .............................................................................................................................................................................. ............................................................................................................................................................................................................. K N E F Y R Q L H S W F I K H Q V F L K E R S D K A N E K G Y F P Y K H R N V R S A Y A S L K R Y M D Y I F T Y E K Y S E L N I E N T T N R L E G L .............................................................................................................................................................................. Subject: UniRef90_A3MYC2 Cluster: Hypothetical protein; n=5; Actinobacillus pleuropneumoniae L20|Rep: Hypothetical protein - Actinobacillus pleuropneumoniae serotype 5b (strain L20) HSP 1 e-value: 4.0E-8 bit: 60.5 Len: 216 Query Start:206 Query End:421 Subject Strand: null Subject Start: 157 Subject End: 230 ............................................................................................................................................................................................................. K N H F Y V L R Y S W Y R N H Q T F L T E R S D K P N E - - N F S Y K Q R N V R S A Y R S S K A I Y E L V I H V * T I C R F R H * K T T N R L K G L .............................................................................................................................................................................. ............................................................................................................................................................................................................. K N E F Y L K I H H W K I K Y K A F L E E R S D K P N E K G K Y P Y K H R N V R S A Y T S I K R Y M D Y I F T Y E K Y P E L N I E K T T N R I E G L .............................................................................................................................................................................. Subject: UniRef90_A3N3G5 Cluster: Hypothetical protein; n=1; Actinobacillus pleuropneumoniae L20|Rep: Hypothetical protein - Actinobacillus pleuropneumoniae serotype 5b (strain L20) HSP 1 e-value: 1.0E-7 bit: 55.8 Len: 153 Query Start:269 Query End:421 Subject Strand: null Subject Start: 95 Subject End: 147 ............................................................................................................................................................................................................................................................................ K N H F Y V L R Y S W Y R N H Q T F L T E R S D K P N E N - - F S Y K Q R N V R S A Y R S S K A I Y E L V .............................................................................................................................................................................. ............................................................................................................................................................................................................................................................................ K N E F Y L R L Y N W K L K H Q D F L N E R S D K Q N E Q G Y F P Y K H R N E R S A Y A S I K R Y M D Y I .............................................................................................................................................................................. HSP 2 e-value: 1.0E-7 bit: 23.1 Len: 39 Query Start:250 Query End:288 Subject Strand: null Subject Start: 141 Subject End: 153 ......................................................................................................................................................................................................................................................... K R Y M N * L F T F E Q Y ................................................................................................................................................................................................................................................................................................................... ......................................................................................................................................................................................................................................................... K R Y M D Y I F T Y E K Y ................................................................................................................................................................................................................................................................................................................... tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntatttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 32 End: 130 ............................... M D R V L T G P V Y L L L F N K Y T G I V D K R V G F T N F F Y L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 79 End: 174 .............................................................................. M Y R H S R Q K S W F Y Q L F L F I R S L I S N T L R I L V K L ..................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 299 End: 571 .......................................................................................................................................................................................................................................................................................................... M R T S D I A L F I R K I L I R L V R A F R Q K R L M I S I P R I A Q D I K V I F L L I N S K K W V N A S Y Y K V L T H ........................ Protein_Len: 37 Strand: + Start: 483 End: 593 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M R Y F S A I P F S R V L K N T I K Y Y R T L G V S I G F C D E V A R K S .. Protein_Len: 30 Strand: - Start: 502 End: 591 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... V R K L P K L F C W L I S G C K L L I S R I R H H L L A F M .... Protein_Len: 32 Strand: - Start: 342 End: 437 ..................................................................................................................................................................................................................................................................................................................................................... E A Q G L T G D F V N S S K S V V F L A P C L L S K K T S L N M .............................................................................................................................................................. Protein_Len: 58 Strand: - Start: 260 End: 433 ................................................................................................................................................................................................................................................................... V H I V L E Y I A K S S R Y A S R V N R Q K Y S F N E N P K D S R E T L F T Q H N R Y W S Y R L V Y F H N K Q Q Y M .................................................................................................................................................................. Protein_Len: 32 Strand: - Start: 114 End: 209 ................................................................................................................. W S K R N I L D R I L L V N R M S T L S H H V A L K Q K L I K M .................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 39 Strand: - Start: 1 End: 117 S L K V S L V G L S M S R T S V P G T Y K N N N L L Y V P M T S L L T P K V M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntatttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntatttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 65 HP_score: -7.1 Tail_Score: -3.22298 Start: 48 End: 91 Full_Region: aactcttttgtctac tatgcctgtatatttatta aacaa taataaatatacaggtcctg ttagtactcgatcga ...............................................tatgcctgtatatttattaaacaataataaatatacaggtcctg........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 67 HP_score: -7.5 Tail_Score: -3.17527 Start: 50 End: 89 Full_Region: ctcttttgtctacta tgcctgtatatttatta aacaa taataaatatacaggtcc tgttagtactcgatc .................................................tgcctgtatatttattaaacaataataaatatacaggtcc.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 95 HP_score: -14.7 Tail_Score: -2.78495 Start: 53 End: 87 Full_Region: cttttgtctactatg cctgtatatttatta aacaa taataaatatacagg tcctgttagtactcg ....................................................cctgtatatttattaaacaataataaatatacagg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 74 HP_score: -6.0 Tail_Score: -4.5954 Start: 102 End: 120 Full_Region: tcttataaatagaaa aagttggt aaa accaactc ttttgtctactatgc .....................................................................................................aagttggtaaaaccaactc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 95 HP_score: -8.6 Tail_Score: -5.24799 Start: 104 End: 118 Full_Region: ATAGTAGACAAAAGA GTTGGT TTT ACCAAC TTTTTCTATTTATAA .......................................................................................................GTTGGTTTTACCAAC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 82 HP_score: -8.6 Tail_Score: -3.90416 Start: 104 End: 118 Full_Region: ttataaatagaaaaa gttggt aaa accaac tcttttgtctactat .......................................................................................................gttggtaaaaccaac............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 43 HP_score: -2.9 Tail_Score: -3.00786 Start: 264 End: 278 Full_Region: CATATTGTTCAAACG TGAAT AACTA ATTCA TATATCGCTTTTGAG .......................................................................................................................................................................................................................................................................TGAATAACTAATTCA............................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 93 HP_score: -9.5 Tail_Score: -4.41206 Start: 442 End: 478 Full_Region: GATAAATTCAAAAAA GTGGGTTAACGCCTCGT ACT ATAAGGTCTTAACCCAC TTTTATCCGCTACTT .........................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGGGTTAACGCCTCGTACTATAAGGTCTTAACCCAC..................................................................................................................... TransTerm Strand: - Conf: 89 HP_score: -7.3 Tail_Score: -5.24449 Start: 442 End: 478 Full_Region: aagtagcggataaaa gtgggttaagaccttat agt acgaggcgttaacccac ttttttgaatttatc .........................................................................................................................................................................................................................................................................................................................................................................................................................................................gtgggttaagaccttatagtacgaggcgttaacccac..................................................................................................................... TransTerm Strand: + Conf: 76 HP_score: -7.7 Tail_Score: -4.07568 Start: 504 End: 516 Full_Region: TACTTTTCTGCTATA CCCTT TTC AAGGG TTTTAAAAAACACCA .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCCTTTTCAAGGG............................................................................... Find igs database================================================ Query_seq: AA00590:AA00591|AA00590:AA00591:outer membrane substrate binding protein; TonB-dependent outer membrane receptor:ABC transporter, ATP-binding protein:->->:403158..403752 595 tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttaccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntatttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta Intra-Species Hit: Count: 1 Min: 1 Max: 595 Len: 595 Subject: aact_AA00590_AA00591|outer membrane substrate binding protein; TonB-dependent outer membrane receptor:ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[403158,403752]|595 HSP 1 e-value: 1.0E-93 bit: 345.0 Len: 174 Query Start:422 Query End:595 Subject Strand: POSITIVE Subject Start: 422 Subject End: 595 .....................................................................................................................................................................................................................................................................................................................................................................................................................................ttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta .....................................................................................................................................................................................................................................................................................................................................................................................................................................ttgttgataaattcaaaaaagtgggttaacgcctcgtactataaggtcttaacccacttttatccgctacttttctgctatacccttttcaagggttttaaaaaacaccataaaatactaccgcactttaggagtatcgatcggattctgtgatgaagtagcgcgaaaatctta HSP 2 e-value: 4.0E-59 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttacca............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tgataatttaactgatagaacacctagagacatcgatcgagtactaacaggacctgtatatttattattgtttaataaatatacaggcatagtagacaaaagagttggttttacca............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAAUUUAACUGAUAGAACACCUAGAGACAUCGAUCGAGUACUAACAGGACCUGUAUAUUUAUUAUUGUUUAAUAAAUAUACAGGCAUAGUAGACAAAAGAGUUGGUUUUACCAACUUUUUCUAUUUAUAAGAUCCCUAAUAAGUAAUACAUUGCGCAUACUUGUCAAACUAUGAUGCACGGCUAAUUUUUGUUUUAAUAUUUUAAUCCCUUAAGUCGAUUAGUUGUUUUUCAAUGCCUAAACCGGCAUAUUGUUCAAACGUGAAUAACUAAUUCAUAUAUCGCUUUUGAGCUCCUAUACGCACUUCUGACAUUGCGCUGUUUAUAAGAAAAAUUCUCAUUCGGCUUGUCCGAGCGUUCCGUCAAAAACGUUUGAUGAUUUCGAUACCACGAAUAGCGCAGGACAUAAAAGUGAUUUUUUUGUUGAUAAAUUCAAAAAAGUGGGUUAACGCCUCGUACUAUAAGGUCUUAACCCACUUUUAUCCGCUACUUUUCUGCUAUACCCUUUUCAAGGGUUUUAAAAAACACCAUAAAAUACUACCGCACUUUAGGAGUAUCGAUCGGAUUCUGUGAUGAAGUAGCGCGAAAAUCUUA (((....(((((.((.(((.(((...((((....((((..((.(((....(.(((((((((((((((.....))))))))))))))))....))))).((((((((((.....))))))))))..........))))........((((.(((..(((((....(((((.....(((...((((((((((((((..((((....)))).....))))..))))))))))...)))(((((......)))))...(((((((.(((((((.........))).)))).)))))))...............))))).)))))))))))).........))))........(((((..(((((.(((((((....)))))))..((((......)))).))))).))))).....))).))).)).))))).....))).(((((((((((((.((((..(...)..)))).))))))))))))).(((((((((((..(((...(((((.....)))))...........((((...(((((.((........)))))))..)).)).....)))..))))))))).))........ (-154.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAUUUUCGCGCUACUUCAUCACAGAAUCCGAUCGAUACUCCUAAAGUGCGGUAGUAUUUUAUGGUGUUUUUUAAAACCCUUGAAAAGGGUAUAGCAGAAAAGUAGCGGAUAAAAGUGGGUUAAGACCUUAUAGUACGAGGCGUUAACCCACUUUUUUGAAUUUAUCAACAAAAAAAUCACUUUUAUGUCCUGCGCUAUUCGUGGUAUCGAAAUCAUCAAACGUUUUUGACGGAACGCUCGGACAAGCCGAAUGAGAAUUUUUCUUAUAAACAGCGCAAUGUCAGAAGUGCGUAUAGGAGCUCAAAAGCGAUAUAUGAAUUAGUUAUUCACGUUUGAACAAUAUGCCGGUUUAGGCAUUGAAAAACAACUAAUCGACUUAAGGGAUUAAAAUAUUAAAACAAAAAUUAGCCGUGCAUCAUAGUUUGACAAGUAUGCGCAAUGUAUUACUUAUUAGGGAUCUUAUAAAUAGAAAAAGUUGGUAAAACCAACUCUUUUGUCUACUAUGCCUGUAUAUUUAUUAAACAAUAAUAAAUAUACAGGUCCUGUUAGUACUCGAUCGAUGUCUCUAGGUGUUCUAUCAGUUAAAUUAUCA ...((((((.((.......((((..(((...(((((((...((((.((((.(((((((.........(((((((((..(((..((((.((.(((..(((.((((((.......(((((((((((((..((((.......))))..)))))))))))))((((.....))))..........)))))).)))..))).)).)))).)))..........((((((((((((((((...(((((((.....))).((((((.....))))))....))))...))))))))...((((((...(((....)))..))))))............)))))))).....(((((......))))))))))))))))).)))))))).))))........................(((((((.(((.....)))...))))).)).......((((...((((.............((((.((((((.....)))))).)))).........((((((((((((((((.....))))))))))))))))))))..)))).)))))))...)))...))))........)).)))..))). (-151.86) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 682 486 441 147 102 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 385 564 521 128 83 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 355 561 522 207 168 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 350 569 522 147 93 aact:AA01024|5end_thiamine_biosynthesis_lipoprotein_precursor_716437..716667_POSITIVE 349 550 502 144 94 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 349 589 541 175 126 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 340 557 514 47 1 aact:AA01041|5end_hypothetical_protein_724853..725083_POSITIVE 339 378 335 62 19 aact:AA01748|5end_thiol:disulfide_interchange_protein_1183062..1183292_POSITIVE 326 551 512 128 93 aact:AA01202|5end_integrase/recombinase_823240..823470_POSITIVE 323 551 514 134 98 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 323 586 541 168 120 aact:AA00272|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III_190406..190636_POSITIVE 319 585 541 159 122 aact:AA01988|5end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1350517..1350747_POSITIVE 319 585 541 57 20 aact:AA01987|5end_hypothetical_protein_1350415..1350645_POSITIVE 313 587 541 152 106 aact:AA01183|5end_conserved_hypothetical_protein_810442..810672_POSITIVE 313 390 341 158 108 aact:AA00170|5end_hypothetical_protein_121238..121468_POSITIVE 312 390 345 57 5 aact:AA00862|5end_conserved_hypothetical_protein_596465..596695_POSITIVE 311 564 521 192 140 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 311 585 541 164 120 aact:AA00249|5end_cysteinyl-tRNA_synthetase_(cysteine--tRNA_ligase)_173966..174196_POSITIVE 311 585 541 66 22 aact:AA00248|5end_hypothetical_protein_173868..174098_POSITIVE 310 559 514 172 130 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 385 564 521 147 102 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 350 569 522 65 11 aact:AA01023|3end_conserved_hypothetical_protein_716435..716585_POSITIVE 349 550 502 72 22 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 340 557 514 60 14 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 339 378 335 50 7 aact:AA01747|3end_single-stranded-DNA-specific_exonuclease_1183130..1183280_POSITIVE 326 551 512 111 76 aact:AA01201|3end_phosphatase;_hydrolase_823303..823453_POSITIVE 323 551 514 43 7 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 323 586 541 151 103 aact:AA00271|3end_hypothetical_protein_190469..190619_POSITIVE 319 585 541 62 25 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE 313 587 541 140 94 aact:AA01182|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_810510..810660_POSITIVE 311 585 541 64 20 aact:AA00247|3end_peptidyl-prolyl_cis-trans_isomerase_B_173946..174096_POSITIVE 310 559 514 147 105 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 309 570 521 109 59 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 308 384 344 89 45 aact:AA01854|3end_conserved_hypothetical_protein_1247089..1247239_POSITIVE 308 565 521 102 58 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 307 385 348 77 39 aact:AA01255|3end_hypothetical_protein_852873..853023_POSITIVE 306 550 507 145 102 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 306 550 507 69 26 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 302 586 541 111 66 aact:AA01954|3end_excinuclease_ABC,_subunit_C_1322314..1322464_POSITIVE 302 379 340 147 111 aact:AA01256|3end_hypothetical_protein_852949..853099_POSITIVE