Origin IGS: atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttcctcctgctgttggcgcattagtataaaggaaatcggcgccctttgccgaccttttgcaggtggcaaaacgccgtaaacttgcgtagaattgccctatcaaaaacgtttgccaaaacgaccgcacttttaggaacaaat Mask Tandem Repeat Region ================================================ atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Find is-nt database================================================ Query_seq: AA00578:AA00579|AA00578:AA00579:DNA topoisomerase:probable DNA recombination protein:->->:395262..395404 143 atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00578:AA00579|AA00578:AA00579:DNA topoisomerase:probable DNA recombination protein:->->:395262..395404 143 atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00578:AA00579|AA00578:AA00579:DNA topoisomerase:probable DNA recombination protein:->->:395262..395404 143 atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 26 End: 118 ......................... M A N V F D R A I L R K F T A F C H L Q K V G K G R R F P L Y ......................... Protein_Len: 47 Strand: + Start: 3 End: 143 .. M F L K V R S F W Q T F L I G Q F Y A S L R R F A T C K R S A K G A D F L Y T N A P T A G G K Protein_Len: 32 Strand: - Start: 1 End: 96 N T G L L A T T K A F T K S L A I R R L N V A N Q W R C F T P M ............................................... atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00578:AA00579|AA00578:AA00579:DNA topoisomerase:probable DNA recombination protein:->->:395262..395404 143 atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 143 Len: 143 Subject: aact_AA00578_AA00579|DNA topoisomerase:probable DNA recombination protein|POSITIVE:POSITIVE|[395262,395404]|143 HSP 1 e-value: 8.0E-76 bit: 283.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa atttgttcctaaaagtgcggtcgttttggcaaacgtttttgatagggcaattctacgcaagtttacggcgttttgccacctgcaaaaggtcggcaaagggcgccgatttcctttatactaatgcgccaacagcaggaggaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUGUUCCUAAAAGUGCGGUCGUUUUGGCAAACGUUUUUGAUAGGGCAAUUCUACGCAAGUUUACGGCGUUUUGCCACCUGCAAAAGGUCGGCAAAGGGCGCCGAUUUCCUUUAUACUAAUGCGCCAACAGCAGGAGGAAAA ..((((.((((.(((.(((...((....))...))).)))..))))))))....((....))...((((((((((((((((.....)))).))))))..)))))).((((((((.......(((.......))))))))))). (-42.61) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCCUCCUGCUGUUGGCGCAUUAGUAUAAAGGAAAUCGGCGCCCUUUGCCGACCUUUUGCAGGUGGCAAAACGCCGUAAACUUGCGUAGAAUUGCCCUAUCAAAAACGUUUGCCAAAACGACCGCACUUUUAGGAACAAAU ..(((((..(((....((.(((....(((.(((.....(((((...((((((.((((.....)))))))))).)))))....)))..)))....))))).........(((((....)))))...))).....)))))..... (-38.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 460 55 11 131 93 aact:AA00860|5end_phosphoheptose_isomerase_591866..592096_POSITIVE 430 108 62 107 51 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 421 136 92 76 24 aact:AA00189|5end_dihydroxynaphthoic_acid_synthase_(naphthoate_synthas)_133119..133349_POSITIVE 412 107 61 125 76 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 403 48 1 159 115 aact:AA00356|5end_DNA-directed_RNA_polymerase,_beta'_chain_238937..239167_POSITIVE 401 59 11 155 113 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 399 110 66 189 138 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 395 59 12 152 99 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 391 49 1 174 128 aact:AA02899|5end_oligopeptide_ABC_transporter,_ATP-binding_protein_2030063..2030293_POSITIVE 391 40 8 182 150 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 383 108 65 167 125 aact:AA01396|5end_saccharide_biosynthesis_regulatory_protein_943760..943990_POSITIVE 381 110 61 77 5 aact:AA02895|5end_oligopeptide_transport_system_permease_protein,_C-terminal_2027484..2027714_POSITIVE 381 102 61 207 154 aact:AA01340|5end_possible_lipoprotein;_possible_HlyD_family_secretion_domain_protein;_possible_membrane_fusion_protein_905746..905976_POSITIVE 379 104 65 90 51 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 377 104 61 170 132 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 376 106 64 133 75 aact:AA01753|5end_conserved_hypothetical_protein_1185322..1185552_POSITIVE 375 110 67 154 109 aact:AA02049|5end_hypothetical_protein_1395526..1395756_POSITIVE 372 58 11 88 38 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 370 104 66 44 13 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 370 40 2 226 188 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 430 108 62 89 33 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 403 48 1 53 9 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 395 59 12 67 14 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 391 40 8 99 67 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 383 108 65 64 22 aact:AA01395|3end_conserved_hypothetical_protein_943737..943887_POSITIVE 379 104 65 87 48 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 376 106 64 123 65 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 375 110 67 127 82 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 374 110 66 57 5 aact:AA02893|3end_oligopeptide_transport_system_permease_protein,_N-terminal_2027544..2027694_POSITIVE 373 45 2 134 90 aact:AA01597|3end_oligoribonuclease_1078936..1079086_POSITIVE 372 58 11 107 57 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 370 104 66 44 13 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 369 90 44 125 73 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 365 99 57 54 1 aact:AA01957|3end_conserved_hypothetical_protein_1323467..1323617_POSITIVE 365 58 13 42 1 aact:AA00450|3end_outer_membrane_antigenic_lipoprotein_b_precursor_315156..315306_POSITIVE 363 50 10 67 27 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 351 43 1 131 89 aact:AA01384|3end_hypothetical_protein_935540..935690_POSITIVE 348 106 66 149 113 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 346 46 2 82 42 aact:AA02616|3end_transcriptional_regulator_1824675..1824825_POSITIVE 340 128 85 98 54 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE