Origin IGS: tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatggatctccgttaagtaaggccaaacgtctaaattagtaaaacttatttaaatcacaaactccagtatctaattagccccttgtaaaaagtgcggttagtttttatcgagttttgggataccctgaagattatagaaacggagaaattcgca Mask Tandem Repeat Region ================================================ tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Find is-nt database================================================ Query_seq: AA00565:AA00566|AA00565:AA00566:30S ribosomal protein S7:30S ribosomal protein S12 (streptomycin resistance protein):->->:390580..390734 155 tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00565:AA00566|AA00565:AA00566:30S ribosomal protein S7:30S ribosomal protein S12 (streptomycin resistance protein):->->:390580..390734 155 tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00565:AA00566|AA00565:AA00566:30S ribosomal protein S7:30S ribosomal protein S12 (streptomycin resistance protein):->->:390580..390734 155 tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Predict ORF larger than 30AA ================================================ tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00565:AA00566|AA00565:AA00566:30S ribosomal protein S7:30S ribosomal protein S12 (streptomycin resistance protein):->->:390580..390734 155 tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: aact_AA00565_AA00566|30S ribosomal protein S7:30S ribosomal protein S12 (streptomycin resistance protein)|POSITIVE:POSITIVE|[390580,390734]|155 HSP 1 e-value: 6.0E-83 bit: 307.0 Len: 155 Query Start:1 Query End:155 Subject Strand: POSITIVE Subject Start: 1 Subject End: 155 tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta tgcgaatttctccgtttctataatcttcagggtatcccaaaactcgataaaaactaaccgcactttttacaaggggctaattagatactggagtttgtgatttaaataagttttactaatttagacgtttggccttacttaacggagatccatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCGAAUUUCUCCGUUUCUAUAAUCUUCAGGGUAUCCCAAAACUCGAUAAAAACUAACCGCACUUUUUACAAGGGGCUAAUUAGAUACUGGAGUUUGUGAUUUAAAUAAGUUUUACUAAUUUAGACGUUUGGCCUUACUUAACGGAGAUCCAUUA ........((((((((..(((((.((((((..((((.........))))....((((..((.((((....)))).))...))))...)))))).))))).(((((((.((.....)).)))))))...............))))))))....... (-27.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGGAUCUCCGUUAAGUAAGGCCAAACGUCUAAAUUAGUAAAACUUAUUUAAAUCACAAACUCCAGUAUCUAAUUAGCCCCUUGUAAAAAGUGCGGUUAGUUUUUAUCGAGUUUUGGGAUACCCUGAAGAUUAUAGAAACGGAGAAAUUCGCA ....(((((((((((.....((((.....))))....................((((..((((((..(((...((((((((((((.....))).).))))))))..))).)))))).(((...)))....)))).....))))))))..)))... (-32.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 330 77 34 104 56 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 320 67 24 104 51 aact:AA01437|5end_phosphoglycerate_kinase_964125..964355_POSITIVE 303 90 42 230 176 aact:AA01462|5end_xylose_operon_regulatory_protein_981530..981760_POSITIVE 303 90 42 112 58 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 299 70 24 154 109 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 297 78 51 171 138 aact:AA02615|5end_conserved_hypothetical_protein_1823040..1823270_POSITIVE 293 70 28 195 149 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 292 93 55 154 122 aact:AA00844|5end_cell_division_protein_I;_penicillin_binding_protein_2X_582802..583032_POSITIVE 291 77 45 171 143 aact:AA02927|5end_hypothetical_protein_2048233..2048463_POSITIVE 291 77 45 74 46 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 286 99 53 145 111 aact:AA00390|5end_permease_263689..263919_POSITIVE 277 76 39 128 95 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 275 67 26 124 78 aact:AA01994|5end_chorismate_mutase;_prephenate_dehydrogenase_1354959..1355189_POSITIVE 271 88 57 163 127 aact:AA02792|5end_GlpT/UhpT_family_transporter_(hexosphosphate_transport)_1959617..1959847_POSITIVE 270 67 22 153 107 aact:AA01737|5end_exoribonuclease_II_1172647..1172877_POSITIVE 270 67 29 113 72 aact:AA00851|5end_carbon_starvation_protein_586760..586990_POSITIVE 268 67 26 216 171 aact:AA00084|5end_aminotransferase_60814..61044_POSITIVE 268 67 26 66 21 aact:AA00083|5end_hypothetical_protein_60664..60894_POSITIVE 267 77 31 100 57 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 263 78 39 86 44 aact:AA02589|5end_NAD(P)H_oxidoreductase_1800493..1800723_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 77 34 87 36 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 317 77 39 44 7 aact:AA01640|3end_restriction_enzyme_type_I_helicase_subunit_1110839..1110989_POSITIVE 303 90 42 112 58 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 303 99 51 110 60 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 299 70 24 149 104 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 297 78 51 83 50 aact:AA02614|3end_conserved_hypothetical_protein_1823032..1823182_POSITIVE 292 93 55 133 101 aact:AA00841|3end_UDP-MurNAc-tripeptide_synthetase_582861..583011_POSITIVE 286 77 39 53 8 aact:AA01436|3end_fructose-bisphosphate_aldolase_class_II_964137..964287_POSITIVE 277 76 39 42 9 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 275 67 26 89 43 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 270 67 22 140 94 aact:AA01735|3end_hypothetical_protein_1172714..1172864_POSITIVE 270 67 29 59 18 aact:AA00849|3end_MraZ_cell_division_protein_586786..586936_POSITIVE 268 67 26 89 44 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 267 77 31 84 41 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 263 78 39 83 41 aact:AA02587|3end_conserved_hypothetical_protein_1800570..1800720_POSITIVE 263 100 55 112 60 aact:AA02126|3end_conserved_hypothetical_protein_1451384..1451534_POSITIVE 263 68 33 71 34 aact:AA01140|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_782526..782676_POSITIVE 262 100 55 124 78 aact:AA00938|3end_L-xylulokinase_648358..648508_POSITIVE 260 68 27 63 26 aact:AA00981|3end_carboxylesterase_686377..686527_POSITIVE 259 66 22 42 3 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE