Origin IGS: tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacaactccgaaaaagtaaatcagggcaatagtatagatcttttaacgtattgcccccaaagggtttaatgcgaaattcaaagaaaaaaatgaccgcacttttgtgcggtcaggtggcta Mask Tandem Repeat Region ================================================ tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Find is-nt database================================================ Query_seq: AA00543:AA00544|AA00543:AA00544:ADP-heptose--LPS heptosyltransferase II:glutathionylspermidine synthetase;amidase:->->:372468..372587 120 tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00543:AA00544|AA00543:AA00544:ADP-heptose--LPS heptosyltransferase II:glutathionylspermidine synthetase;amidase:->->:372468..372587 120 tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00543:AA00544|AA00543:AA00544:ADP-heptose--LPS heptosyltransferase II:glutathionylspermidine synthetase;amidase:->->:372468..372587 120 tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 30 End: 119 ............................. M F F F E F R I K P F G G N T L K D L Y Y C P D L L F R S C . tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00543:AA00544|AA00543:AA00544:ADP-heptose--LPS heptosyltransferase II:glutathionylspermidine synthetase;amidase:->->:372468..372587 120 tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Intra-Species Hit: Count: 1 Min: 1 Max: 120 Len: 120 Subject: aact_AA00543_AA00544|ADP-heptose--LPS heptosyltransferase II:glutathionylspermidine synthetase;amidase|POSITIVE:POSITIVE|[372468,372587]|120 HSP 1 e-value: 1.0E-49 bit: 196.0 Len: 120 Query Start:1 Query End:120 Subject Strand: POSITIVE Subject Start: 1 Subject End: 120 tagccacctgaccgcacaaaagtgcggtcannnnnnnctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt tagccacctgaccgcacaaaagtgcggtcatttttttctttgaatttcgcattaaaccctttgggggcaatacgttaaaagatctatactattgccctgatttactttttcggagttgtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCACCUGACCGCACAAAAGUGCGGUCAUUUUUUUCUUUGAAUUUCGCAUUAAACCCUUUGGGGGCAAUACGUUAAAAGAUCUAUACUAUUGCCCUGAUUUACUUUUUCGGAGUUGUU ........(((((((((....)))))))))............(((((((............(.(((((((((.((............))))))))))).)..........)))))))... (-33.05) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAACUCCGAAAAAGUAAAUCAGGGCAAUAGUAUAGAUCUUUUAACGUAUUGCCCCCAAAGGGUUUAAUGCGAAAUUCAAAGAAAAAAAUGACCGCACUUUUGUGCGGUCAGGUGGCUA .....(.((...........((.((((((((((.((((...)))))).)))))))).....((((((......))))))...))......(((((((((....))))))))))).).... (-32.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 38 10 147 119 aact:AA02348|5end_bacterioferritin_comigratory_protein_1606772..1607002_POSITIVE 372 49 11 145 104 aact:AA00148|5end_ATP-dependent_RNA_helicase_105955..106185_POSITIVE 371 38 3 175 140 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 371 50 2 165 104 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 370 68 21 209 162 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 356 68 21 62 15 aact:AA00482|5end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_335232..335462_POSITIVE 350 66 21 140 81 aact:AA02610|5end_acyl-CoA_thioesterase_II_1820371..1820601_POSITIVE 346 47 9 107 68 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 345 69 21 116 50 aact:AA01271|5end_hypothetical_protein_863794..864024_POSITIVE 343 50 3 103 50 aact:AA01745|5end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180394..1180624_POSITIVE 339 48 14 160 123 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 338 68 23 148 98 aact:AA00237|5end_hypothetical_protein_170934..171164_POSITIVE 335 35 3 173 145 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 333 42 5 102 62 aact:AA00618|5end_molybdopterin_biosynthesis_protein_422984..423214_POSITIVE 322 50 5 216 158 aact:AA01175|5end_6-phosphogluconate_dehydrogenase_804864..805094_POSITIVE 322 42 6 140 103 aact:AA00705|5end_Inositol-1-monophosphatase_(IMPase);_extragenic_suppressor_483827..484057_POSITIVE 319 50 2 201 153 aact:AA03008|5end_GTP-binding_protein_era_homolog_(GTPase)_2100087..2100317_POSITIVE 318 47 12 137 102 aact:AA02465|5end_glucosamine-6-phosphate_isomerase_1704005..1704235_POSITIVE 318 47 12 42 7 aact:AA02464|5end_hypothetical_protein_1703910..1704140_POSITIVE 317 50 2 179 115 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 38 10 77 49 aact:AA02347|3end_immunoglobulin_A1_protease_precursor_1606782..1606932_POSITIVE 372 49 11 54 13 aact:AA00146|3end_UDP-glucose/GDP-mannose_dehydrogenase_105944..106094_POSITIVE 371 38 3 70 35 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 371 50 2 142 81 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 370 68 21 108 61 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 345 69 21 139 73 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 334 67 21 77 26 aact:AA01804|3end_sugar_nucleotide_epimerase;_cell_division_inhibitor_1216001..1216151_POSITIVE 325 57 11 100 49 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 322 42 6 81 44 aact:AA00702|3end_conserved_hypothetical_protein_483848..483998_POSITIVE 318 47 12 146 111 aact:AA02464|3end_hypothetical_protein_1704094..1704244_POSITIVE 317 50 2 96 32 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 317 68 21 130 74 aact:AA00302|3end_DNA_polymerase_III,_delta'_subunit_206205..206355_POSITIVE 315 49 7 67 27 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 305 46 2 80 26 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 305 70 21 99 51 aact:AA01460|3end_hypothetical_protein_981596..981746_POSITIVE 305 50 6 94 51 aact:AA01090|3end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_756213..756363_POSITIVE 303 30 4 56 27 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 301 68 21 68 12 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 301 50 2 121 66 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 301 50 2 137 82 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE