Origin IGS: agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaatttccattgtaataatttcctatcatcactttgaaactacaacaaaaatacactattttgtgctaagctgactcctttgagatataatagcgccaaggtattattctgaacatgttgctaaaacgtgaggttcacaatgaaaaaaaagattcattccgtgttatttttgcttacatatttttttagtattcaaggacaaact Mask Tandem Repeat Region ================================================ agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Find is-nt database================================================ Query_seq: AA00537:AA00538|AA00537:AA00538:conserved hypothetical protein:hypothetical protein:->->:368840..369045 206 agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00537:AA00538|AA00537:AA00538:conserved hypothetical protein:hypothetical protein:->->:368840..369045 206 agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00537:AA00538|AA00537:AA00538:conserved hypothetical protein:hypothetical protein:->->:368840..369045 206 agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Predict ORF larger than 30AA ================================================ agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 144 End: 173 ...............................................................................................................................................TTTGAAACTACAACAAAAATACACTATTTT................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00537:AA00538|AA00537:AA00538:conserved hypothetical protein:hypothetical protein:->->:368840..369045 206 agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Intra-Species Hit: Count: 1 Min: 1 Max: 206 Len: 206 Subject: aact_AA00537_AA00538|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[368840,369045]|206 HSP 1 e-value: 2.0E-86 bit: 319.0 Len: 206 Query Start:1 Query End:206 Subject Strand: POSITIVE Subject Start: 1 Subject End: 206 agtttgtccttgaatactnnnnnnntatgtaagcaaaaataacacggaatgaatcnnnnnnnncattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca agtttgtccttgaatactaaaaaaatatgtaagcaaaaataacacggaatgaatcttttttttcattgtgaacctcacgttttagcaacatgttcagaataataccttggcgctattatatctcaaaggagtcagcttagcacaaaatagtgtatttttgttgtagtttcaaagtgatgataggaaattattacaatggaaattca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUUGUCCUUGAAUACUAAAAAAAUAUGUAAGCAAAAAUAACACGGAAUGAAUCUUUUUUUUCAUUGUGAACCUCACGUUUUAGCAACAUGUUCAGAAUAAUACCUUGGCGCUAUUAUAUCUCAAAGGAGUCAGCUUAGCACAAAAUAGUGUAUUUUUGUUGUAGUUUCAAAGUGAUGAUAGGAAAUUAUUACAAUGGAAAUUCA .(....(((.((((.((((............((((((((((.(((..((((((........)))))).(((((.....((....)).....)))))..........(((..((((......(((....)))......)))).)))....))))))))))))).))))))))..((((((((.....))))))))...)))....). (-37.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUUUCCAUUGUAAUAAUUUCCUAUCAUCACUUUGAAACUACAACAAAAAUACACUAUUUUGUGCUAAGCUGACUCCUUUGAGAUAUAAUAGCGCCAAGGUAUUAUUCUGAACAUGUUGCUAAAACGUGAGGUUCACAAUGAAAAAAAAGAUUCAUUCCGUGUUAUUUUUGCUUACAUAUUUUUUUAGUAUUCAAGGACAAACU ................................(((((((((((.(((((((((((((.(((((((((((......(((....)))......))))).)))))).(((((.((((((((((.....)))))...))))).))))).................))).))))))))...........)).)))).)))))))....... (-32.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 85 44 111 70 aact:AA02430|5end_conserved_hypothetical_protein_1675251..1675481_POSITIVE 287 145 104 178 137 aact:AA00420|5end_tRNA/rRNA_methyltransferase_284953..285183_POSITIVE 272 117 73 63 9 aact:AA02482|5end_conserved_hypothetical_protein_1720131..1720361_POSITIVE 262 120 73 174 121 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 259 111 75 206 175 aact:AA01729|5end_hypothetical_protein_1168585..1168815_POSITIVE 257 115 76 231 193 aact:AA02037|5end_hypothetical_protein_1386832..1387062_POSITIVE 257 113 73 206 162 aact:AA00086|5end_2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy_late_synthase_63437..63667_POSITIVE 255 114 84 227 197 aact:AA00137|5end_conserved_hypothetical_protein_(possible_alpha_helix_protein)_98447..98677_POSITIVE 254 112 75 117 76 aact:AA02440|5end_ABC_transporter,_ATP-binding_protein_1685043..1685273_POSITIVE 254 149 104 62 24 aact:AA00981|5end_carboxylesterase_685245..685475_POSITIVE 252 141 105 177 150 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 251 116 77 155 124 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 249 89 41 58 11 aact:AA00088|5end_30S_ribosomal_protein_S15_66041..66271_POSITIVE 241 49 1 188 138 aact:AA01347|5end_conserved_hypothetical_protein_913209..913439_POSITIVE 240 108 61 177 127 aact:AA01233|5end_50S_ribosomal_protein_L6_840853..841083_POSITIVE 236 143 104 230 196 aact:AA02961|5end_pyrroline-5-carboxylate_reductase_2070785..2071015_POSITIVE 234 149 104 208 151 aact:AA01899|5end_conserved_hypothetical_protein_1281253..1281483_POSITIVE 234 143 107 179 143 aact:AA01400|5end_phosphopantetheine_adenylyltransferase_945679..945909_POSITIVE 231 109 64 103 50 aact:AA00915|5end_xanthine-guanine_phosphoribosyltransferase_629610..629840_POSITIVE 231 118 76 175 132 aact:AA00737|5end_citrate_lyase,_alpha_chain_(citrase)_503564..503794_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 287 145 104 56 15 aact:AA00419|3end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284911..285061_POSITIVE 262 120 73 103 50 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 255 114 84 151 121 aact:AA00136|3end_sodium/sulphate_transporter_98451..98601_POSITIVE 254 112 75 112 71 aact:AA02439|3end_integral_membrane_protein;_possible_ABC_transporter_permease_1685118..1685268_POSITIVE 254 149 104 146 108 aact:AA00980|3end_hypothetical_protein_685409..685559_POSITIVE 236 137 94 89 47 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 236 137 94 89 47 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 234 143 107 111 75 aact:AA01398|3end_KDO_kinase_945691..945841_POSITIVE 229 118 71 146 96 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 229 138 97 146 94 aact:AA01897|3end_conserved_hypothetical_protein_1281284..1281434_POSITIVE 228 86 44 147 97 aact:AA00037|3end_conserved_hypothetical_protein_29906..30056_POSITIVE 227 176 137 141 95 aact:AA02748|3end_conserved_hypothetical_protein_1925582..1925732_POSITIVE 224 146 109 49 19 aact:AA01305|3end_conserved_hypothetical_protein_886520..886670_POSITIVE 223 103 67 71 28 aact:AA00171|3end_DNA-binding_protein_121873..122023_POSITIVE 218 123 84 135 102 aact:AA01490|3end_ATP_synthase_F0,_subunit_a_1002369..1002519_POSITIVE 217 112 73 124 82 aact:AA00205|3end_ribose_ABC_transporter_protein_/_high_affinity_D-ribose_transport_protein_142658..142808_POSITIVE 214 122 85 136 94 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 213 136 106 125 91 aact:AA00904|3end_sigma-E_factor_negative_regulatory_factor_625104..625254_POSITIVE 212 185 150 59 26 aact:AA02703|3end_hypothetical_protein_1885582..1885732_POSITIVE 209 136 110 109 83 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE