Origin IGS: taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaaaaaagttgaccgcactttgatctgcccccaaaagttggacaggtcagttaactgaagtattgagctcggtattgtaccaggctcaatacttttaaatcgagttta Mask Tandem Repeat Region ================================================ taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Find is-nt database================================================ Query_seq: AA00535:AA00536|AA00535:AA00536:hypothetical protein:5'-nucleotidase precursor:->->:366180..366290 111 taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00535:AA00536|AA00535:AA00536:hypothetical protein:5'-nucleotidase precursor:->->:366180..366290 111 taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00535:AA00536|AA00535:AA00536:hypothetical protein:5'-nucleotidase precursor:->->:366180..366290 111 taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 19 End: 111 .................. M E P G T I P S S I L Q L T D L S N F W G Q I K V R S T F L L taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -14.3 Tail_Score: -4.20106 Start: 17 End: 49 Full_Region: ggtcagttaactgaa gtattgagctcggta ttg taccaggctcaatac ttttaaatcgagttt ................gtattgagctcggtattgtaccaggctcaatac.............................................................. Find igs database================================================ Query_seq: AA00535:AA00536|AA00535:AA00536:hypothetical protein:5'-nucleotidase precursor:->->:366180..366290 111 taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: aact_AA00535_AA00536|hypothetical protein:5'-nucleotidase precursor|POSITIVE:POSITIVE|[366180,366290]|111 HSP 1 e-value: 7.0E-57 bit: 220.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta taaactcgatttaaaagtattgagcctggtacaataccgagctcaatacttcagttaactgacctgtccaacttttgggggcagatcaaagtgcggtcaacttttttgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACUCGAUUUAAAAGUAUUGAGCCUGGUACAAUACCGAGCUCAAUACUUCAGUUAACUGACCUGUCCAACUUUUGGGGGCAGAUCAAAGUGCGGUCAACUUUUUUGUUA .......(((....(((((((((((.(((((...))))).)))))))))))..((....(((.((((((.........)))))).)))....)).)))............. (-31.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAAAAAAGUUGACCGCACUUUGAUCUGCCCCCAAAAGUUGGACAGGUCAGUUAACUGAAGUAUUGAGCUCGGUAUUGUACCAGGCUCAAUACUUUUAAAUCGAGUUUA .(((.....(((((((.......(((((((...(((.....))).))))))))))))))((((((((((((..((((...))))..))))))))))))........))).. (-34.71) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 734 80 32 116 68 aact:AA01444|5end_ISRS011/IS150_related_transposase,_orfB_969159..969389_POSITIVE 734 80 32 116 68 aact:AA01287|5end_transposase_871583..871813_POSITIVE 679 70 21 150 101 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 652 108 64 151 105 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 606 98 65 58 24 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 580 97 65 81 48 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 535 97 60 74 32 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 504 108 65 139 98 aact:AA01075|5end_hypothetical_protein_745605..745835_POSITIVE 489 100 65 148 112 aact:AA01267|5end_degenerate_transposase_fragment_862267..862497_POSITIVE 456 97 57 146 109 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 437 97 65 131 98 aact:AA02340|5end_hypothetical_protein_1598887..1599117_POSITIVE 412 51 15 227 191 aact:AA01407|5end_outer_membrane_antigenic_lipoprotein_b_precursor_949005..949235_POSITIVE 412 51 15 229 193 aact:AA00632|5end_inner_membrane_protein_ImpA_434127..434357_POSITIVE 369 109 61 170 123 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 364 97 54 47 1 aact:AA02629|5end_hypothetical_protein_1830072..1830302_POSITIVE 361 98 57 147 109 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 356 99 52 224 180 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 339 108 61 176 127 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 331 100 60 66 13 aact:AA00303|5end_thymidylate_kinase_(DTMP)_206107..206337_POSITIVE 330 108 63 218 171 aact:AA00717|5end_methionyl-tRNA_synthetase_491304..491534_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 734 80 32 70 22 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 606 98 65 64 30 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 580 97 65 63 30 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 535 97 60 74 32 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 504 108 65 77 36 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 489 100 65 52 16 aact:AA01266|3end_hypothetical_protein_862251..862401_POSITIVE 464 99 65 41 8 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 456 97 57 60 23 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 437 97 65 49 16 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 434 99 64 61 27 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 369 109 61 76 29 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 361 98 57 59 21 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 356 99 52 73 29 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 339 108 61 71 22 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 339 51 15 76 40 aact:AA00535|3end_hypothetical_protein_366119..366269_POSITIVE 331 100 60 84 31 aact:AA00302|3end_DNA_polymerase_III,_delta'_subunit_206205..206355_POSITIVE 330 108 63 78 31 aact:AA00716|3end_conserved_hypothetical_protein_491244..491394_POSITIVE 319 99 57 113 72 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 315 98 63 47 11 aact:AA00455|3end_lipoprotein_precursor_319385..319535_POSITIVE 314 98 60 57 22 aact:AA01703|3end_L-ribulose-phosphate_4-epimerase_1156161..1156311_POSITIVE