Origin IGS: agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaccagcaccttataattaggtgttcaggttttgggtgcagatcacttttttattaaacactgctgcccgttttttcaaccaccaaaatccgcgcttcgccctgcgggtggacgacgtgttcgatgccgatgccggcgtaaaaaatatctgctttccggcaatacgcttctggcttcgct Mask Tandem Repeat Region ================================================ agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Find is-nt database================================================ Query_seq: AA00534:AA00535|AA00534:AA00535:hypothetical protein:hypothetical protein:->->:365655..365834 180 agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00534:AA00535|AA00534:AA00535:hypothetical protein:hypothetical protein:->->:365655..365834 180 agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00534:AA00535|AA00534:AA00535:hypothetical protein:hypothetical protein:->->:365655..365834 180 agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 35 End: 124 .................................. M F Y A G I G I E H V V H P Q G E A R I L V V E K T G S S V ........................................................ Protein_Len: 48 Strand: + Start: 18 End: 161 ................. M A G K Q I F F T P A S A S N T S S T R R A K R G F W W L K K R A A V F N K K V I C T Q N L N T ................... Protein_Len: 49 Strand: - Start: 3 End: 149 .. S A L L T N G S L L Y K K R R C R C R V R R G G A P R L A S K P P Q F F P C C H K I F F H D A G M ............................... Protein_Len: 53 Strand: - Start: 2 End: 160 . R L W F R I A P F C I N K V G A D A D F V D D V R L A F R P N Q H N F F R A A T N L L F T I Q V W F R F M .................... agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00534:AA00535|AA00534:AA00535:hypothetical protein:hypothetical protein:->->:365655..365834 180 agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Intra-Species Hit: Count: 1 Min: 1 Max: 180 Len: 180 Subject: aact_AA00534_AA00535|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[365655,365834]|180 HSP 1 e-value: 8.0E-98 bit: 357.0 Len: 180 Query Start:1 Query End:180 Subject Strand: POSITIVE Subject Start: 1 Subject End: 180 agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt agcgaagccagaagcgtattgccggaaagcagatattttttacgccggcatcggcatcgaacacgtcgtccacccgcagggcgaagcgcggattttggtggttgaaaaaacgggcagcagtgtttaataaaaaagtgatctgcacccaaaacctgaacacctaattataaggtgctggtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCGAAGCCAGAAGCGUAUUGCCGGAAAGCAGAUAUUUUUUACGCCGGCAUCGGCAUCGAACACGUCGUCCACCCGCAGGGCGAAGCGCGGAUUUUGGUGGUUGAAAAAACGGGCAGCAGUGUUUAAUAAAAAAGUGAUCUGCACCCAAAACCUGAACACCUAAUUAUAAGGUGCUGGUU .....((((((.((.((.(((..((...((((((.(((((((.((((....))))...((((((.((((((.......)))))).((.((..((((.(....).))))..)).))....))))))..)))))))...)))))).))))).))))...(((((.......))))))))))) (-54.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCAGCACCUUAUAAUUAGGUGUUCAGGUUUUGGGUGCAGAUCACUUUUUUAUUAAACACUGCUGCCCGUUUUUUCAACCACCAAAAUCCGCGCUUCGCCCUGCGGGUGGACGACGUGUUCGAUGCCGAUGCCGGCGUAAAAAAUAUCUGCUUUCCGGCAAUACGCUUCUGGCUUCGCU (((((((((((.......)))))))..))))..((((((((...................)))).)))).....................((((((((((((...))))))).).))))..(((.(((((((((((.(((.........)))...)))))).......)).))).))).. (-48.72) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 497 87 43 59 19 aact:AA00715|5end_[2Fe-2S]_ferredoxin_490632..490862_POSITIVE 485 180 136 165 121 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 481 180 134 214 169 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 461 89 43 74 23 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 449 89 46 50 5 aact:AA00475|5end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_331621..331851_POSITIVE 440 100 51 177 118 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 440 90 46 51 6 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 434 90 46 62 12 aact:AA00384|5end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_260108..260338_POSITIVE 434 89 44 126 71 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 432 90 43 206 165 aact:AA00918|5end_hypothetical_protein_630044..630274_POSITIVE 430 83 43 74 31 aact:AA00488|5end_purine_nucleoside_phosphorylase_338075..338305_POSITIVE 429 90 41 91 26 aact:AA01772|5end_shikimate_5-dehydrogenase_protein_1194050..1194280_POSITIVE 428 180 136 163 111 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 424 90 41 61 4 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 424 90 43 198 132 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 423 100 53 80 28 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 423 88 43 229 185 aact:AA00932|5end_ribose_ABC_transporter,_periplasmic_protein_643177..643407_POSITIVE 420 89 43 103 68 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 418 88 44 205 134 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 417 89 43 79 41 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 503 90 43 78 19 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 497 87 43 44 4 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 489 180 131 87 28 aact:AA01549|3end_hypothetical_protein_1037880..1038030_POSITIVE 485 180 136 104 60 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 481 180 134 90 45 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 461 89 43 62 11 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 449 89 46 73 28 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 434 90 46 56 6 aact:AA00383|3end_chloride_channel_protein_260182..260332_POSITIVE 420 89 43 65 30 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 411 118 73 55 19 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 409 90 42 66 13 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 405 100 51 106 38 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 401 87 43 65 15 aact:AA02366|3end_hypothetical_protein_1623252..1623402_POSITIVE 396 100 51 68 9 aact:AA02470|3end_glucose_kinase_1707694..1707844_POSITIVE 390 88 45 67 20 aact:AA01201|3end_phosphatase;_hydrolase_823303..823453_POSITIVE 388 90 43 82 27 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 385 90 43 68 6 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 383 60 12 147 106 aact:AA01417|3end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_954028..954178_POSITIVE 379 89 43 149 108 aact:AA02950|3end_isoleucyl-tRNA_synthetase_2065113..2065263_POSITIVE 379 88 43 70 15 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE