Origin IGS: ttcccacgccttggcgtgacttggcatggtgtcgataaccgtgccatccatgtcaaaaattaaaccgtcatattggtttattacatcattatctaacatattgccttccggattgtcttagttacaacaaataagatagctattgtgcaataaatttatgatccgtgcatcgtttaatttcggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttacctgctctcacctaaagctgactaagtataaccgcataccgccgaaattaaacgatgcacggatcataaatttattgcacaatagctatcttatttgttgtaactaagacaatccggaaggcaatatgttagataatgatgtaataaaccaatatgacggtttaatttttgacatggatggcacggttatcgacaccatgccaagtcacgccaaggcgtgggaa Mask Tandem Repeat Region ================================================ ttcccacgccttggcgtgacttggcatggtgtcgataaccgtgccatccatgtcaaaaattaaaccgtcatattggtttattacatcattatctaacatattgccttccggattgtcttagttacaacaaataagatagctattgtgcaataaatttatgatccgtgcatcgtttaatttcggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Find is-nt database================================================ Query_seq: AA00521:AA00523|AA00521:AA00523:phosphatase:conserved hypothetical protein:->->:358385..358611 227 ttcccacgccttggcgtgacttggcatggtgtcgataaccgtgccatccatgtcaaaaattaaaccgtcatattggtttattacatcattatctaacatattgccttccggattgtcttagttacaacaaataagatagctattgtgcaataaatttatgatccgtgcatcgtttaatttcggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00521:AA00523|AA00521:AA00523:phosphatase:conserved hypothetical protein:->->:358385..358611 227 ttcccacgccttggcgtgacttggcatggtgtcgataaccgtgccatccatgtcaaaaattaaaccgtcatattggtttattacatcattatctaacatattgccttccggattgtcttagttacaacaaataagatagctattgtgcaataaatttatgatccgtgcatcgtttaatttcggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00521:AA00523|AA00521:AA00523:phosphatase:conserved hypothetical protein:->->:358385..358611 227 ttcccacgccttggcgtgacttggcatggtgtcgataaccgtgccatccatgtcaaaaattaaaccgtcatattggtttattacatcattatctaacatattgccttccggattgtcttagttacaacaaataagatagctattgtgcaataaatttatgatccgtgcatcgtttaatttcggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 1 Max: 181 Len: 181 Subject: UniRef90_Q65VI7 Cluster: Hypothetical protein; n=1; Mannheimia succiniciproducens MBEL55E|Rep: Hypothetical protein - Mannheimia succiniciproducens (strain MBEL55E) HSP 1 e-value: 3.0E-9 bit: 59.3 Len: 99 Query Start:1 Query End:99 Subject Strand: null Subject Start: 48 Subject End: 80 M L D N D V I N Q Y D G L I F D M D G T V I D T M P S H A K A W E ................................................................................................................................ M Y D L I T M N Q Y D A L I F D M D G T I I D T M P S H A K A W E ................................................................................................................................ HSP 2 e-value: 3.0E-9 bit: 23.9 Len: 39 Query Start:143 Query End:181 Subject Strand: null Subject Start: 26 Subject End: 38 .............................................................................................................................................. E I K R C T D H K F I A Q .............................................. .............................................................................................................................................. K I K K C I D H K F I G K .............................................. nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattgccttccggattgtcttagttacaacaaataagatagctannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 26 End: 118 ......................... M V S I T V P S M S K I K P S Y W F I T S L S N I L P S G L S ............................................................................................................. Protein_Len: 33 Strand: - Start: 1 End: 99 E W A K A H S P M T D I V T G D M D F I L G D Y Q N I V D N D L M ................................................................................................................................ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattgccttccggattgtcttagttacaacaaataagatagctannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattgccttccggattgtcttagttacaacaaataagatagctannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 44 HP_score: -2.9 Tail_Score: -3.15677 Start: 61 End: 80 Full_Region: ATCCATGTCAAAAAT TAAACCG TCATAT TGGTTTA TTACATCATTATCTA ............................................................TAAACCGTCATATTGGTTTA................................................................................................................................................... TransTerm Strand: - Conf: 45 HP_score: -2.2 Tail_Score: -3.37188 Start: 61 End: 80 Full_Region: tagataatgatgtaa taaacc aatatgac ggttta atttttgacatggat ............................................................taaaccaatatgacggttta................................................................................................................................................... Find igs database================================================ Query_seq: AA00521:AA00523|AA00521:AA00523:phosphatase:conserved hypothetical protein:->->:358385..358611 227 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattgccttccggattgtcttagttacaacaaataagatagctannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggcggtatgcggttatacttagtcagctttaggtgagagcaggtaa Intra-Species Hit: Count: 1 Min: 100 Max: 227 Len: 128 Subject: aact_AA00521_AA00523|phosphatase:conserved hypothetical protein|POSITIVE:POSITIVE|[358385,358611]|227 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 44 Query Start:184 Query End:227 Subject Strand: POSITIVE Subject Start: 184 Subject End: 227 .......................................................................................................................................................................................cggtatgcggttatacttagtcagctttaggtgagagcaggtaa .......................................................................................................................................................................................cggtatgcggttatacttagtcagctttaggtgagagcaggtaa HSP 2 e-value: 6.0E-16 bit: 85.7 Len: 43 Query Start:100 Query End:142 Subject Strand: POSITIVE Subject Start: 100 Subject End: 142 ...................................................................................................attgccttccggattgtcttagttacaacaaataagatagcta..................................................................................... ...................................................................................................attgccttccggattgtcttagttacaacaaataagatagcta..................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGCCUUCCGGAUUGUCUUAGUUACAACAAAUAAGAUAGCUAUUGUGCAAUAAAUUUAUGAUCCGUGCAUCGUUUAAUUUCGGCGGUAUGCGGUUAUACUUAGUCAGCUUUAGGUGAGAGCAGGUAA .(((((....((.((((((((...........))))))))))....(((........(((((.((((..((((((.......))))))..))))))))).....(((.(....).)))..)))))))) (-27.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUACCUGCUCUCACCUAAAGCUGACUAAGUAUAACCGCAUACCGCCGAAAUUAAACGAUGCACGGAUCAUAAAUUUAUUGCACAAUAGCUAUCUUAUUUGUUGUAACUAAGACAAUCCGGAAGGCAAU ...(((((((.........(((.....)))....(.((.....)).).........)).))).))......................(((.(((...(((((........)))))...))).)))... (-16.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 335 117 71 132 73 aact:AA00033|5end_conserved_hypothetical_protein_25643..25873_POSITIVE 330 108 65 70 18 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 330 109 66 201 156 aact:AA01248|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849734..849964_POSITIVE 330 109 66 131 86 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 329 108 64 166 125 aact:AA02928|5end_conserved_hypothetical_protein_2048361..2048591_POSITIVE 329 107 65 66 23 aact:AA02217|5end_deoxyribose-phosphate_aldolase_1509135..1509365_POSITIVE 325 95 59 190 145 aact:AA00807|5end_fumarate_reductase_13_kD_hydrophobic_protein_552767..552997_POSITIVE 320 95 60 37 1 aact:AA01213|5end_competence_related_protein_829867..830097_POSITIVE 319 96 56 216 174 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 314 99 65 77 42 aact:AA01360|5end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_921047..921277_POSITIVE 312 110 63 148 98 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 307 115 72 52 18 aact:AA02455|5end_conserved_hypothetical_protein_1694353..1694583_POSITIVE 305 109 65 78 26 aact:AA02242|5end_molybdenum_cofactor_biosynthesis_protein_A_1523998..1524228_POSITIVE 300 86 42 212 159 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 300 95 61 221 190 aact:AA02763|5end_DNA_polymerase_III,_epsilon_subunit_1933696..1933926_POSITIVE 300 107 61 194 135 aact:AA02346|5end_hypothetical_protein_1603819..1604049_POSITIVE 299 120 71 81 22 aact:AA01663|5end_GTP_cyclohydrolase_I_1127471..1127701_POSITIVE 294 110 63 83 36 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 294 97 52 75 23 aact:AA00373|5end_CoA-transferase_(beta)_251013..251243_POSITIVE 293 97 60 82 40 aact:AA00837|5end_cell_division_protein_W_576317..576547_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 335 117 71 126 67 aact:AA00031|3end_conserved_hypothetical_protein_25717..25867_POSITIVE 330 109 66 68 23 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE 329 108 64 130 89 aact:AA02927|3end_hypothetical_protein_2048405..2048555_POSITIVE 329 108 64 72 31 aact:AA02926|3end_hypothetical_protein_2048347..2048497_POSITIVE 314 99 65 59 24 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 312 110 63 119 69 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 308 109 62 151 115 aact:AA01292|3end_single-stranded_DNA_binding_protein_876383..876533_POSITIVE 307 115 72 52 18 aact:AA02454|3end_ATP-dependent_helicase_1694433..1694583_POSITIVE 306 94 53 142 104 aact:AA01496|3end_glucose_inhibited_division_protein_A_1007091..1007241_POSITIVE 305 109 65 62 10 aact:AA02241|3end_molybdenum_cofactor_biosynthesis_protein_C_1524062..1524212_POSITIVE 294 97 52 60 8 aact:AA00372|3end_acetate_CoA-transferase_(alpha_subunit)_251078..251228_POSITIVE 282 107 64 87 40 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 279 108 63 79 29 aact:AA02552|3end_iron_(chelated)_ABC_transporter,_permease_protein_1779420..1779570_POSITIVE 276 94 57 29 2 aact:AA02703|3end_hypothetical_protein_1885582..1885732_POSITIVE 275 87 44 80 37 aact:AA02626|3end_conserved_hypothetical_protein_1829202..1829352_POSITIVE 275 120 81 68 26 aact:AA00831|3end_cell_division_protein_Q_572849..572999_POSITIVE 274 109 66 151 98 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 273 98 58 60 6 aact:AA02573|3end_ABC_transporter,_ATP-binding_protein_1792429..1792579_POSITIVE 272 107 63 148 107 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 270 110 64 59 1 aact:AA01426|3end_threonine_deaminase_(dehydratase)_958655..958805_POSITIVE