Origin IGS: attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaagcctgcgattttcctgtaaaatacataaaaaaaatgcctctcacgaagagaggcaaataacctaaggaaccaattttattaaaacaactgcaagttgctcgtaatttatgacaatagtataacggaaaaagttcgcaagaatttacaaaaacttataaataatttacgaacgcttaatttttaatcaaaaaagctaaaattgaggaaaaaat Mask Tandem Repeat Region ================================================ attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Find is-nt database================================================ Query_seq: AA00516:AA00518|AA00516:AA00518:autoinducer-2 production protein:conserved hypothetical protein:->->:356575..356790 216 attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00516:AA00518|AA00516:AA00518:autoinducer-2 production protein:conserved hypothetical protein:->->:356575..356790 216 attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00516:AA00518|AA00516:AA00518:autoinducer-2 production protein:conserved hypothetical protein:->->:356575..356790 216 attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Predict ORF larger than 30AA ================================================ attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 72 HP_score: -6.3 Tail_Score: -4.28361 Start: 116 End: 128 Full_Region: GTCATAAATTACGAG CAACT TGC AGTTG TTTTAATAAAATTGG ...................................................................................................................CAACTTGCAGTTG........................................................................................ TransTerm Strand: + Conf: 100 HP_score: -11.2 Tail_Score: -6.08563 Start: 158 End: 178 Full_Region: GTTCCTTAGGTTATT TGCCTCTC TTCGT GAGAGGCA TTTTTTTTATGTATT .............................................................................................................................................................TGCCTCTCTTCGTGAGAGGCA...................................... Find igs database================================================ Query_seq: AA00516:AA00518|AA00516:AA00518:autoinducer-2 production protein:conserved hypothetical protein:->->:356575..356790 216 attttttcctcaattttagcttttttgattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Intra-Species Hit: Count: 1 Min: 27 Max: 216 Len: 190 Subject: aact_AA00516_AA00518|autoinducer-2 production protein:conserved hypothetical protein|POSITIVE:POSITIVE|[356575,356790]|216 HSP 1 e-value: 2.0E-89 bit: 329.0 Len: 190 Query Start:27 Query End:216 Subject Strand: POSITIVE Subject Start: 27 Subject End: 216 ..........................gattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcannnnnnnnatgtattttacaggaaaatcgcaggcttta ..........................gattaaaaattaagcgttcgtaaattatttataagtttttgtaaattcttgcgaactttttccgttatactattgtcataaattacgagcaacttgcagttgttttaataaaattggttccttaggttatttgcctctcttcgtgagaggcattttttttatgtattttacaggaaaatcgcaggcttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUAAAAAUUAAGCGUUCGUAAAUUAUUUAUAAGUUUUUGUAAAUUCUUGCGAACUUUUUCCGUUAUACUAUUGUCAUAAAUUACGAGCAACUUGCAGUUGUUUUAAUAAAAUUGGUUCCUUAGGUUAUUUGCCUCUCUUCGUGAGAGGCAUUUUUUUUAUGUAUUUUACAGGAAAAUCGCAGGCUUUA ...........((((((((((((...((((((((....))))))))..)))))))).((((((((.((((...........((((.(((((((.....))))))))))).............((((..(..((((((((.....))))))))..)..)))).))))...)).))))))......)))).. (-43.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGCCUGCGAUUUUCCUGUAAAAUACAUAAAAAAAAUGCCUCUCACGAAGAGAGGCAAAUAACCUAAGGAACCAAUUUUAUUAAAACAACUGCAAGUUGCUCGUAAUUUAUGACAAUAGUAUAACGGAAAAAGUUCGCAAGAAUUUACAAAAACUUAUAAAUAAUUUACGAACGCUUAAUUUUUAAUC ........(((...((((((((...)))..........((((((((.....)))))))).........)))))...............((((.....)))).((((((.((((......(((..........((((((....))))))......))).....)))).)))))).)))............. (-35.29) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 128 83 109 69 aact:AA01324|5end_50S_ribosomal_protein_L33_894495..894725_POSITIVE 259 151 116 160 131 aact:AA00822|5end_conserved_hypothetical_protein_(possible_arsenate_reductase)_564596..564826_POSITIVE 251 156 115 158 119 aact:AA00631|5end_hypothetical_protein_433678..433908_POSITIVE 250 189 141 158 101 aact:AA01846|5end_conserved_hypothetical_protein_1241843..1242073_POSITIVE 249 154 114 84 47 aact:AA00512|5end_conserved_hypothetical_protein_354411..354641_POSITIVE 247 151 125 147 122 aact:AA00113|5end_uracil_phosphoribosyltransferase_81286..81516_POSITIVE 240 119 85 85 42 aact:AA00890|5end_conserved_hypothetical_protein_617075..617305_POSITIVE 239 151 114 176 132 aact:AA01988|5end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1350517..1350747_POSITIVE 239 151 114 74 30 aact:AA01987|5end_hypothetical_protein_1350415..1350645_POSITIVE 239 189 141 146 95 aact:AA00303|5end_thymidylate_kinase_(DTMP)_206107..206337_POSITIVE 236 164 125 142 108 aact:AA02456|5end_conserved_hypothetical_protein_1694743..1694973_POSITIVE 235 93 51 56 2 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 235 176 134 169 127 aact:AA00803|5end_hypothetical_protein_549526..549756_POSITIVE 234 186 141 199 153 aact:AA01662|5end_molybdopterin_biosynthesis_protein_1126131..1126361_POSITIVE 234 90 41 100 41 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 234 164 125 215 186 aact:AA00929|5end_DNA_gyrase_subunit_B_637797..638027_POSITIVE 233 154 115 60 18 aact:AA00468|5end_tryptophan-specific_transport_protein_/_tyrosine-specific_transport_protein_324803..325033_POSITIVE 230 57 12 55 10 aact:AA01922|5end_translation_initiation_factor_IF2_1294710..1294940_POSITIVE 229 150 102 146 88 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 227 151 115 184 149 aact:AA01404|5end_hypothetical_protein_948578..948808_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 128 83 94 54 aact:AA01323|3end_50S_ribosomal_protein_L28_894560..894710_POSITIVE 262 158 114 73 23 aact:AA00020|3end_conserved_hypothetical_protein_14486..14636_POSITIVE 259 160 116 51 6 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 249 154 114 75 38 aact:AA00511|3end_nicotinamide_mononucleotide_transporter_354482..354632_POSITIVE 239 151 114 79 35 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE 236 164 125 138 104 aact:AA02455|3end_conserved_hypothetical_protein_1694819..1694969_POSITIVE 235 176 134 102 60 aact:AA00801|3end_lysine--tRNA_ligase_549539..549689_POSITIVE 234 90 41 84 25 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 234 164 125 115 86 aact:AA00927|3end_ATP-dependent_DNA_helicase_637777..637927_POSITIVE 233 154 115 55 13 aact:AA00467|3end_hypothetical_protein_324878..325028_POSITIVE 232 151 113 150 103 aact:AA01918|3end_anaerobic_C4-dicarboxylate_transporter_1292360..1292510_POSITIVE 227 151 128 20 1 aact:AA00112|3end_uracil_permease_81239..81389_POSITIVE 226 150 115 67 27 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 222 96 51 118 65 aact:AA00987|3end_tetracenomycin_polyketide_synthesis_O-methyltransferase_690586..690736_POSITIVE 221 152 120 148 112 aact:AA02270|3end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1548668..1548818_POSITIVE 220 121 81 79 45 aact:AA01235|3end_30S_ribosomal_protein_S5_842346..842496_POSITIVE 218 151 122 131 102 aact:AA02415|3end_hypothetical_protein_1658726..1658876_POSITIVE 218 159 123 108 73 aact:AA01952|3end_ABC_transporter,_substrate_binding_protein_1320155..1320305_POSITIVE 218 119 90 40 7 aact:AA00889|3end_conserved_hypothetical_protein_617110..617260_POSITIVE 218 156 119 135 96 aact:AA00101|3end_fimbrial_biogenesis_and_twitching_motility_protein_74410..74560_POSITIVE