Origin IGS: cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaacgctaattggcacattttgagcaagtcaacatagaattggcaaacgccattcgcgcaaaactcgcctaattcaaatttaactcaaggaccggccgcacttttgtctcaaaagtgcggtcacaaaaaattccgttttttgaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttttagatgaatttcactttccttagaaaaaatgtgtaaaattacattttttcgttggaaaaagtgtatctaattgacattttttccttagaaaaaatgtaaagtctgcgttagaatagcgagaaatccaataagaggatcaacg Mask Tandem Repeat Region ================================================ cgttgatcctcttattggatttctcgctattctaacgcagacttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Find is-nt database================================================ Query_seq: AA00504:AA00505|AA00504:AA00505:ATPase:hypothetical protein:->->:351348..351700 353 cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00504:AA00505|AA00504:AA00505:ATPase:hypothetical protein:->->:351348..351700 353 cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00504:AA00505|AA00504:AA00505:ATPase:hypothetical protein:->->:351348..351700 353 cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 19 End: 120 .................. M S R Y S N A D F T F F L R K K C Q L D T L F P T K K C N F T H F F ......................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 174 End: 269 ............................................................................................................................................................................. M H P F G A V G I A N V Q K T E F F V T A L L R Q K C G R S L S .................................................................................... Protein_Len: 41 Strand: + Start: 158 End: 280 ............................................................................................................................................................. M T E V I Y S P L R G R W H S Q R S K N G I F C D R T F E T K V R P V L E L N L N ......................................................................... cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 29 End: 58 ............................TTAGAAAAAATGTAAAGTCTGCGTTAGAAT....................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 74 HP_score: -3.0 Tail_Score: -5.26875 Start: 100 End: 111 Full_Region: tccttagaaaaaatg tgta aaat taca ttttttcgttggaaa ...................................................................................................tgtaaaattaca.................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 79 HP_score: -11.3 Tail_Score: -2.79456 Start: 228 End: 260 Full_Region: AAAACGGAATTTTTT GTGACCGCACTTTTG AGA CAAAAGTGCGGCCGG TCCTTGAGTTAAATT ...................................................................................................................................................................................................................................GTGACCGCACTTTTGAGACAAAAGTGCGGCCGG............................................................................................. Find igs database================================================ Query_seq: AA00504:AA00505|AA00504:AA00505:ATPase:hypothetical protein:->->:351348..351700 353 cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Intra-Species Hit: Count: 1 Min: 1 Max: 353 Len: 353 Subject: aact_AA00504_AA00505|ATPase:hypothetical protein|POSITIVE:POSITIVE|[351348,351700]|353 HSP 1 e-value: 0.0 bit: 658.0 Len: 353 Query Start:1 Query End:353 Subject Strand: POSITIVE Subject Start: 1 Subject End: 353 cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctnnnnnnntattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta cgttgatcctcttattggatttctcgctattctaacgcagactttacattttttctaaggaaaaaatgtcaattagatacactttttccaacgaaaaaatgtaattttacacattttttctaaggaaagtgaaattcatctaaaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacggaattttttgtgaccgcacttttgagacaaaagtgcggccggtccttgagttaaatttgaattaggcgagttttgcgcgaatggcgtttgccaattctatgttgacttgctcaaaatgtgccaattagcgttcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUGAUCCUCUUAUUGGAUUUCUCGCUAUUCUAACGCAGACUUUACAUUUUUUCUAAGGAAAAAAUGUCAAUUAGAUACACUUUUUCCAACGAAAAAAUGUAAUUUUACACAUUUUUUCUAAGGAAAGUGAAAUUCAUCUAAAAAAAUAUUCAUAAAUCACUGAAGUGAUUUAUUCACCCCUUCGGGGCCGUUGGCAUAGCCAACGUUCAAAAAACGGAAUUUUUUGUGACCGCACUUUUGAGACAAAAGUGCGGCCGGUCCUUGAGUUAAAUUUGAAUUAGGCGAGUUUUGCGCGAAUGGCGUUUGCCAAUUCUAUGUUGACUUGCUCAAAAUGUGCCAAUUAGCGUUCUA ((..(((((.......)))))...)).......(((((.....((((((((((((...(((((((.((((.....))))...)))))))...)))))))))))).....((((((((....(((((....(((((((................(((((((((....)))))))))....(((....))).(((((((...)))))))...........)))))))...((.(((((((((((...))))))))))).)).)))))(((((((....(((((.(((((.....((((.....))))))))))))))....)))))))....)))))))).......)))))... (-104.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAACGCUAAUUGGCACAUUUUGAGCAAGUCAACAUAGAAUUGGCAAACGCCAUUCGCGCAAAACUCGCCUAAUUCAAAUUUAACUCAAGGACCGGCCGCACUUUUGUCUCAAAAGUGCGGUCACAAAAAAUUCCGUUUUUUGAACGUUGGCUAUGCCAACGGCCCCGAAGGGGUGAAUAAAUCACUUCAGUGAUUUAUGAAUAUUUUUUUAGAUGAAUUUCACUUUCCUUAGAAAAAAUGUGUAAAAUUACAUUUUUUCGUUGGAAAAAGUGUAUCUAAUUGACAUUUUUUCCUUAGAAAAAAUGUAAAGUCUGCGUUAGAAUAGCGAGAAAUCCAAUAAGAGGAUCAACG .....(((((...(((...(((((.((...((......))..((((....))))...)).)))))...))).........((((((....((((.(((((((((((((...))))))))))))).((((((((...))))))))..(((((((...)))))))..(((....)))...(((((((((....)))))))))..((((((((((.((.(((.......))).)).))))))))))......((((((((((((...(((((((((((.........)))))))))))...)))))))))))).))))..)))))).)))))....((((.......))))..... (-108.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 882 197 151 231 185 aact:AA00096|5end_L-asparaginase_II_precursor_71280..71510_POSITIVE 877 218 171 185 138 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 774 218 175 208 164 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 702 218 175 64 20 aact:AA01953|5end_hypothetical_protein_1320132..1320362_POSITIVE 692 209 166 133 89 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 592 218 171 218 173 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 547 215 175 165 125 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 473 240 191 220 171 aact:AA01502|5end_serine_acetyltransferase_1010014..1010244_POSITIVE 450 229 182 183 138 aact:AA02728|5end_conserved_hypothetical_protein_(possible_ATPase)_1910145..1910375_POSITIVE 423 259 215 196 148 aact:AA02486|5end_topoisomerase_IV,_subunit_B_1724337..1724567_POSITIVE 392 256 212 141 93 aact:AA02536|5end_conserved_hypothetical_protein_1764854..1765084_POSITIVE 391 328 283 153 102 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 390 260 211 58 10 aact:AA01041|5end_hypothetical_protein_724853..725083_POSITIVE 380 329 282 117 53 aact:AA00117|5end_hypothetical_protein_88246..88476_POSITIVE 379 240 200 45 4 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 369 240 191 168 122 aact:AA02867|5end_ribonuclease_T_2013118..2013348_POSITIVE 360 183 149 231 196 aact:AA00702|5end_conserved_hypothetical_protein_481660..481890_POSITIVE 356 330 283 213 162 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 350 329 283 104 62 aact:AA02442|5end_thioredoxin-family_protein_1685704..1685934_POSITIVE 347 240 196 176 132 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 980 209 161 56 8 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 729 310 263 118 71 aact:AA00223|3end_catalase_(hydroperoxidase;_iron_heme_axial_ligand)_155808..155958_POSITIVE 692 209 166 81 37 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 592 218 171 88 43 aact:AA01934|3end_conserved_hypothetical_protein_1306993..1307143_POSITIVE 590 218 177 40 1 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 547 215 175 134 94 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 470 240 193 49 1 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 461 240 191 73 25 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE 457 218 175 144 101 aact:AA01501|3end_hypothetical_protein_1010040..1010190_POSITIVE 453 256 212 75 26 aact:AA02208|3end_tryptophan_synthase,__alpha_subunit_1503996..1504146_POSITIVE 437 240 191 86 37 aact:AA02913|3end_ribosomal_large_subunit_pseudouridine_synthase_2040527..2040677_POSITIVE 392 256 212 68 20 aact:AA02534|3end_zinc_protease_1764861..1765011_POSITIVE 391 328 283 85 34 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 390 260 211 71 23 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 388 258 212 47 1 aact:AA01952|3end_ABC_transporter,_substrate_binding_protein_1320155..1320305_POSITIVE 380 240 192 66 17 aact:AA00663|3end_cystathionine_beta-lyase_455934..456084_POSITIVE 369 240 191 78 32 aact:AA02866|3end_lactoylglutathione_lyase_2013108..2013258_POSITIVE 356 330 283 115 64 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 350 329 283 111 69 aact:AA02440|3end_ABC_transporter,_ATP-binding_protein_1685791..1685941_POSITIVE 347 240 196 72 28 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE