Origin IGS: taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttagtttattccatggatttattataaattgtagaatattgtttaaaaattctactgattagtcaggaagacaataactttataaattagttcagtcacaattta Mask Tandem Repeat Region ================================================ taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Find is-nt database================================================ Query_seq: AA00489:AA00490|AA00489:AA00490:hypothetical protein:possible outer membrane protein (hemin-binding protein / haemin storage protein):->->:339093..339200 108 taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00489:AA00490|AA00489:AA00490:hypothetical protein:possible outer membrane protein (hemin-binding protein / haemin storage protein):->->:339093..339200 108 taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00489:AA00490|AA00489:AA00490:hypothetical protein:possible outer membrane protein (hemin-binding protein / haemin storage protein):->->:339093..339200 108 taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Predict ORF larger than 30AA ================================================ taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 56 End: 85 .......................................................ATTTTTAAACAATATTCTACAATTTATAAT....................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00489:AA00490|AA00489:AA00490:hypothetical protein:possible outer membrane protein (hemin-binding protein / haemin storage protein):->->:339093..339200 108 taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 108 Len: 108 Subject: aact_AA00489_AA00490|hypothetical protein:possible outer membrane protein (hemin-binding protein / haemin storage protein)|POSITIVE:POSITIVE|[339093,339200]|108 HSP 1 e-value: 4.0E-55 bit: 214.0 Len: 108 Query Start:1 Query End:108 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa taaattgtgactgaactaatttataaagttattgtcttcctgactaatcagtagaatttttaaacaatattctacaatttataataaatccatggaataaactaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUGUGACUGAACUAAUUUAUAAAGUUAUUGUCUUCCUGACUAAUCAGUAGAAUUUUUAAACAAUAUUCUACAAUUUAUAAUAAAUCCAUGGAAUAAACUAAAAA ....((((..(.........(((((((......(((.....)))......(((((((...........)))))))..))))))).........)..))))........ (-13.07) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUAGUUUAUUCCAUGGAUUUAUUAUAAAUUGUAGAAUAUUGUUUAAAAAUUCUACUGAUUAGUCAGGAAGACAAUAACUUUAUAAAUUAGUUCAGUCACAAUUUA ................((((((..(((((((..(((((((.((.....)).)))))))......(((.....)))......)))))))...))))))........... (-15.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 214 46 5 156 121 aact:AA00442|5end_conserved_hypothetical_protein_(possible_zinc_finger_protein)_306189..306419_POSITIVE 196 47 11 202 166 aact:AA00869|5end_tight_adherence_protein_V;_prepilin_peptidase_601255..601485_POSITIVE 179 75 36 193 150 aact:AA01903|5end_hypothetical_protein_1283983..1284213_POSITIVE 177 44 8 117 78 aact:AA00131|5end_transcriptional_regulator_(MerR_family)_94521..94751_POSITIVE 177 44 4 108 68 aact:AA00101|5end_fimbrial_biogenesis_and_twitching_motility_protein_73782..74012_POSITIVE 174 52 29 220 193 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 169 55 21 208 170 aact:AA01758|5end_hydrogenase_isoenzymes_nickel_incorporation_protein_1187839..1188069_POSITIVE 164 44 4 159 118 aact:AA01989|5end_hypothetical_protein_1352039..1352269_POSITIVE 162 49 27 27 5 aact:AA00024|5end_transcriptional_regulatory_protein_19011..19241_POSITIVE 160 56 26 76 47 aact:AA02305|5end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1574778..1575008_POSITIVE 158 58 15 135 90 aact:AA01662|5end_molybdopterin_biosynthesis_protein_1126131..1126361_POSITIVE 158 57 27 181 153 aact:AA00432|5end_conserved_hypothetical_protein;_possible_methyltransferase_293897..294127_POSITIVE 154 55 15 67 26 aact:AA02331|5end_ABC_transporter,_ATP-binding_protein;_possible_leukotoxin_secretion_ATP-binding_protein_1591955..1592185_POSITIVE 151 54 29 118 93 aact:AA01479|5end_spermidine/putrescine-binding_periplasmic_protein_994705..994935_POSITIVE 149 75 36 224 179 aact:AA01400|5end_phosphopantetheine_adenylyltransferase_945679..945909_POSITIVE 148 70 27 153 106 aact:AA02906|5end_hypothetical_protein_2033304..2033534_POSITIVE 147 43 6 153 106 aact:AA03014|5end_conserved_hypothetical_protein_2104815..2105045_POSITIVE 142 59 25 77 41 aact:AA00886|5end_hypothetical_protein_616584..616814_POSITIVE 141 56 25 160 125 aact:AA01292|5end_single-stranded_DNA_binding_protein_875827..876057_POSITIVE 141 53 11 162 99 aact:AA00608|5end_protective_surface_antigen_D-15,_85_-kilodalton_outermembrane_protein_413161..413391_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 177 44 8 68 29 aact:AA00129|3end_hypothetical_protein_94552..94702_POSITIVE 177 44 4 83 43 aact:AA00099|3end_conserved_hypothetical_protein_73837..73987_POSITIVE 174 52 29 146 119 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 169 55 21 126 88 aact:AA01757|3end_cobalt_transport_ATP-binding_protein_1187837..1187987_POSITIVE 168 50 33 135 114 aact:AA01556|3end_ABC_transporter_ATP-binding_protein_1044759..1044909_POSITIVE 164 44 4 58 17 aact:AA01988|3end_dihydrolipoamide_dehydrogenase;_lipoamide_dehydrogenase_1352018..1352168_POSITIVE 160 56 26 72 43 aact:AA02303|3end_ATP-dependent_DNA_helicase_1574854..1575004_POSITIVE 158 58 15 116 71 aact:AA01661|3end_molybdopterin_biosynthesis_protein_1126192..1126342_POSITIVE 154 72 35 138 98 aact:AA00716|3end_conserved_hypothetical_protein_491244..491394_POSITIVE 151 54 29 113 88 aact:AA01478|3end_hypothetical_protein_994780..994930_POSITIVE 148 53 12 123 69 aact:AA00811|3end_conserved_hypothetical_protein_558027..558177_POSITIVE 147 43 6 104 57 aact:AA03013|3end_ribonuclease_P_protein_component_2104846..2104996_POSITIVE 145 79 31 63 13 aact:AA02199|3end_hypothetical_protein_1497901..1498051_POSITIVE 144 52 27 151 127 aact:AA02905|3end_conserved_hypothetical_protein_2033405..2033555_POSITIVE 142 43 6 131 90 aact:AA00940|3end_probable_transcriptional_regulator_(LysR_family)_651828..651978_POSITIVE 142 59 25 72 36 aact:AA00885|3end_ribosomal_large_subunit_pseudouridine_synthase_c_616659..616809_POSITIVE 141 53 11 140 77 aact:AA00606|3end_membrane-associated_zinc_metalloprotease_413219..413369_POSITIVE 140 52 36 40 25 aact:AA01067|3end_30S_ribosomal_protein_S2_739697..739847_POSITIVE 139 57 19 147 113 aact:AA02743|3end_outer_membrane_lipoprotein_precursor/glutamyl-tRNA_reductase_subunit_1923356..1923506_POSITIVE 137 61 41 113 92 aact:AA01564|3end_transaldolase_B_1050129..1050279_POSITIVE