Origin IGS: taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtgttttccttttattacattacgttgaacaaaattctaagcaaacgttttcgctacctataaacgacaacaccacgaacgcgtcgtggtgtcgtcaaaaaacgtcgttaaatcataccgcacttta Mask Tandem Repeat Region ================================================ taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Find is-nt database================================================ Query_seq: AA00486:AA00488|AA00486:AA00488:nucleoside transporter:purine nucleoside phosphorylase:->->:338087..338214 128 taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00486:AA00488|AA00486:AA00488:nucleoside transporter:purine nucleoside phosphorylase:->->:338087..338214 128 taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00486:AA00488|AA00486:AA00488:nucleoside transporter:purine nucleoside phosphorylase:->->:338087..338214 128 taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Predict ORF larger than 30AA ================================================ taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -15.0 Tail_Score: -3.91897 Start: 31 End: 63 Full_Region: TTTAACGACGTTTTT TGACGACACCACGA CGCGT TCGTGGTGTTGTCG TTTATAGGTAGCGAA ..............................TGACGACACCACGACGCGTTCGTGGTGTTGTCG................................................................. TransTerm Strand: + Conf: 100 HP_score: -16.3 Tail_Score: -2.94924 Start: 32 End: 62 Full_Region: TTAACGACGTTTTTT GACGACACCACGA CGCGT TCGTGGTGTTGTC GTTTATAGGTAGCGA ...............................GACGACACCACGACGCGTTCGTGGTGTTGTC.................................................................. TransTerm Strand: - Conf: 62 HP_score: -8.0 Tail_Score: -2.78384 Start: 39 End: 55 Full_Region: ctataaacgacaaca ccacga acgcg tcgtgg tgtcgtcaaaaaacg ......................................ccacgaacgcgtcgtgg......................................................................... Find igs database================================================ Query_seq: AA00486:AA00488|AA00486:AA00488:nucleoside transporter:purine nucleoside phosphorylase:->->:338087..338214 128 taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: aact_AA00486_AA00488|nucleoside transporter:purine nucleoside phosphorylase|POSITIVE:POSITIVE|[338087,338214]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact taaagtgcggtatgatttaacgacgttttttgacgacaccacgacgcgttcgtggtgttgtcgtttataggtagcgaaaacgtttgcttagaattttgttcaacgtaatgtaataaaaggaaaacact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGUGCGGUAUGAUUUAACGACGUUUUUUGACGACACCACGACGCGUUCGUGGUGUUGUCGUUUAUAGGUAGCGAAAACGUUUGCUUAGAAUUUUGUUCAACGUAAUGUAAUAAAAGGAAAACACU .....((((.((((.((.(((((.((((...(((((((((((((.....))))))))))))).....(((((((((....)).))))))))))).))))).)))))).))))................ (-35.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGUUUUCCUUUUAUUACAUUACGUUGAACAAAAUUCUAAGCAAACGUUUUCGCUACCUAUAAACGACAACACCACGAACGCGUCGUGGUGUCGUCAAAAAACGUCGUUAAAUCAUACCGCACUUUA (((((......((((....((.(((((.............(((..........)))..........(((.(((((((((.....))))))))).)))....))))).)))))).......)))))... (-26.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 383 89 41 220 180 aact:AA00414|5end_possible_histidinol-phosphatase_280899..281129_POSITIVE 376 90 42 123 79 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 357 57 13 200 152 aact:AA01202|5end_integrase/recombinase_823240..823470_POSITIVE 355 77 31 137 92 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 351 77 34 212 161 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 346 88 41 127 82 aact:AA00718|5end_ABC-related_ATP-binding_protein;_MRP_protein_493534..493764_POSITIVE 343 76 34 215 175 aact:AA02300|5end_glutamate_racemase_1571348..1571578_POSITIVE 343 76 34 220 180 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 341 50 1 194 148 aact:AA02177|5end_conserved_hypothetical_protein_1488963..1489193_POSITIVE 340 48 1 141 92 aact:AA02034|5end_ferredoxin_NADP+_reductase_1385198..1385428_POSITIVE 338 47 1 97 44 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 337 60 14 171 121 aact:AA00179|5end_conserved_hypothetical_protein_(possible_outer_membrane_protein)_126260..126490_POSITIVE 335 60 16 197 150 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 334 65 21 49 4 aact:AA02190|5end_hydrogenase-2_component_protein_1492919..1493149_POSITIVE 329 76 34 231 181 aact:AA02439|5end_integral_membrane_protein;_possible_ABC_transporter_permease_1683902..1684132_POSITIVE 329 77 37 202 159 aact:AA00612|5end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_417840..418070_POSITIVE 328 63 21 231 191 aact:AA02670|5end_hypothetical_protein_1858049..1858279_POSITIVE 327 89 41 57 6 aact:AA01523|5end_conserved_hypothetical_protein_1023205..1023435_POSITIVE 318 62 21 162 117 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 314 77 33 225 183 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 490 62 24 59 15 aact:AA00486|3end_nucleoside_transporter_338026..338176_POSITIVE 376 90 42 69 25 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 355 77 31 134 89 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 341 50 1 117 71 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 338 47 1 109 56 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 337 60 14 144 94 aact:AA00177|3end_conserved_hypothetical_protein_126313..126463_POSITIVE 314 66 21 115 83 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 313 49 2 109 55 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 311 50 1 84 40 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 311 89 42 51 1 aact:AA01522|3end_conserved_hypothetical_protein_1023279..1023429_POSITIVE 310 65 23 144 104 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 306 63 26 111 69 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 303 50 2 64 10 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 303 63 21 74 38 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 303 90 45 95 48 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 303 41 2 107 62 aact:AA01013|3end_phosphomannomutase_709470..709620_POSITIVE 303 47 2 86 44 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 302 50 2 61 10 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 300 50 2 80 37 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 300 90 46 151 104 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE