Origin IGS: taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttatcgccttacggttattagagaataaaacagcaatccctaacagcacaaaaataccgataatgctaattaaaatgtccataagaacctcaaagtagattaaaattctgcctattctattgtttcccataaaaaaatgggaacgaatttta Mask Tandem Repeat Region ================================================ taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Find is-nt database================================================ Query_seq: AA00485:AA00486|AA00485:AA00486:hypothetical protein:nucleoside transporter:->->:336769..336922 154 taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00485:AA00486|AA00485:AA00486:hypothetical protein:nucleoside transporter:->->:336769..336922 154 taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00485:AA00486|AA00485:AA00486:hypothetical protein:nucleoside transporter:->->:336769..336922 154 taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: + Start: 5 End: 154 .... M R S H F F M G N N R I G R I L I Y F E V L M D I L I S I I G I F V L L G I A V L F S N N R K A I N Protein_Len: 31 Strand: - Start: 58 End: 150 ......................................................... K S T R I S M K I L M I P I K T S N P I A T K N E L L R L A M .... taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00485:AA00486|AA00485:AA00486:hypothetical protein:nucleoside transporter:->->:336769..336922 154 taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Intra-Species Hit: Count: 1 Min: 1 Max: 154 Len: 154 Subject: aact_AA00485_AA00486|hypothetical protein:nucleoside transporter|POSITIVE:POSITIVE|[336769,336922]|154 HSP 1 e-value: 8.0E-70 bit: 264.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 154 taaaattcgttcccannnnnnnatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat taaaattcgttcccatttttttatgggaaacaatagaataggcagaattttaatctactttgaggttcttatggacattttaattagcattatcggtatttttgtgctgttagggattgctgttttattctctaataaccgtaaggcgataaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUCGUUCCCAUUUUUUUAUGGGAAACAAUAGAAUAGGCAGAAUUUUAAUCUACUUUGAGGUUCUUAUGGACAUUUUAAUUAGCAUUAUCGGUAUUUUUGUGCUGUUAGGGAUUGCUGUUUUAUUCUCUAAUAACCGUAAGGCGAUAAAU ((((((((.(((((((......)))))))..............((((((((((......))))))))))....)).)))))).......((((((............(((((((((.((......)).))))))))).((....)))))))).. (-32.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUAUCGCCUUACGGUUAUUAGAGAAUAAAACAGCAAUCCCUAACAGCACAAAAAUACCGAUAAUGCUAAUUAAAAUGUCCAUAAGAACCUCAAAGUAGAUUAAAAUUCUGCCUAUUCUAUUGUUUCCCAUAAAAAAAUGGGAACGAAUUUUA .......(((....)))......((((((....(((((((....................)))..))))...........................(((((.......))))).)))))).(((((.(((((......))))))))))...... (-20.85) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 124 83 154 115 aact:AA02226|5end_ABC_transporter_ATP-binding_protein_1514502..1514732_POSITIVE 290 127 87 59 21 aact:AA01738|5end_enoyl-(acyl-carrier-protein)_reductase_1174699..1174929_POSITIVE 285 141 101 230 179 aact:AA01259|5end_protoporphyrinogen_oxidase_854354..854584_POSITIVE 280 132 91 42 3 aact:AA01028|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_E_718923..719153_POSITIVE 265 122 87 160 119 aact:AA00381|5end_ornithine_carbamoyltransferase_257470..257700_POSITIVE 255 123 95 184 153 aact:AA02832|5end_outer_membrane_integrity_protein_1990044..1990274_POSITIVE 254 119 86 164 130 aact:AA01525|5end_amino_acid_ABC_transporter,_permease_component_1024277..1024507_POSITIVE 253 149 101 171 120 aact:AA02375|5end_D-3-phosphoglycerate_dehydrogenase_1626514..1626744_POSITIVE 253 122 82 100 46 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 251 149 105 162 115 aact:AA01089|5end_hypothetical_protein_753392..753622_POSITIVE 250 135 93 165 115 aact:AA01846|5end_conserved_hypothetical_protein_1241843..1242073_POSITIVE 247 143 105 35 1 aact:AA00434|5end_VgrG-like_protein_(Rhs_accessory_genetic_element)_294825..295055_POSITIVE 243 124 92 213 184 aact:AA00544|5end_glutathionylspermidine_synthetase;amidase_372448..372678_POSITIVE 239 148 101 104 50 aact:AA01111|5end_conserved_hypothetical_protein_764765..764995_POSITIVE 238 127 87 93 54 aact:AA02263|5end_conserved_hypothetical_protein_1539092..1539322_POSITIVE 237 148 104 49 6 aact:AA00709|5end_metallocluster_assembly_(NifU_protein)_487378..487608_POSITIVE 235 147 105 194 146 aact:AA02670|5end_hypothetical_protein_1858049..1858279_POSITIVE 233 150 105 57 3 aact:AA00900|5end_conserved_hypothetical_protein_623442..623672_POSITIVE 233 150 103 51 4 aact:AA00737|5end_citrate_lyase,_alpha_chain_(citrase)_503564..503794_POSITIVE 233 148 101 175 115 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 265 122 87 147 106 aact:AA00380|3end_carbamate_kinase_257537..257687_POSITIVE 255 123 95 146 115 aact:AA02830|3end_colicin_tolerance_protein_1990086..1990236_POSITIVE 254 119 86 151 117 aact:AA01524|3end_amino_acid_ABC_transporter,_ATP-binding_protein_1024344..1024494_POSITIVE 253 149 101 149 98 aact:AA02374|3end_ribose_5-phosphate_isomerase_A_1626572..1626722_POSITIVE 252 130 81 144 101 aact:AA02097|3end_DedA-family_integral_membrane_protein_1429505..1429655_POSITIVE 243 124 92 92 63 aact:AA00543|3end_ADP-heptose--LPS_heptosyltransferase_II_372407..372557_POSITIVE 243 149 102 137 80 aact:AA00087|3end_possible_hydrolase_(alpha/beta_fold_family)_66022..66172_POSITIVE 239 126 84 121 72 aact:AA01371|3end_rod_shape-determining_protein_927711..927861_POSITIVE 237 149 102 76 27 aact:AA03012|3end_conserved_hypothetical_protein_2104556..2104706_POSITIVE 236 121 100 67 44 aact:AA01331|3end_50S_ribosomal_protein_L31_898721..898871_POSITIVE 226 143 104 56 23 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 225 134 94 49 5 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 224 148 104 112 51 aact:AA00993|3end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694999..695149_POSITIVE 222 27 1 73 48 aact:AA02249|3end_thiamine_biosynthesis_protein_1529899..1530049_POSITIVE 221 150 106 147 104 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 220 116 72 143 99 aact:AA01303|3end_competence_protein_A_882882..883032_POSITIVE 219 118 74 150 106 aact:AA01818|3end_high-affinity_zinc_uptake_system_membrane_protein_1225078..1225228_POSITIVE 219 125 88 41 10 aact:AA01747|3end_single-stranded-DNA-specific_exonuclease_1183130..1183280_POSITIVE 218 123 93 149 116 aact:AA01393|3end_ABC_transporter,_ATP-binding_protein_942029..942179_POSITIVE 217 149 113 60 22 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE