Origin IGS: taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcaaaacctcctttaagttattaaaaattagacgtttgaatcgtttcagcgaaaaattcaaaacggtcgcaaacgtttgcatgatggctaagtgtattcatttgatgaaagtcgtcaaatagagagttcaaaaatcgtgatcacgatcaacaagtacgatgagttgatagcattaaaaacaacatcttggcgctgaaatgttattttatgtgggggaaatttaagaatttgttatgaaaatcttggtgaaagaggttgcgtttacagcgtcgcctattaaacccgttta Mask Tandem Repeat Region ================================================ taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Find is-nt database================================================ Query_seq: AA00475:AA00478|AA00475:AA00478:conserved hypothetical protein (possible N6-adenine-specific methylase):mannose-specific phosphotransferase element:->->:332343..332631 289 taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00475:AA00478|AA00475:AA00478:conserved hypothetical protein (possible N6-adenine-specific methylase):mannose-specific phosphotransferase element:->->:332343..332631 289 taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00475:AA00478|AA00475:AA00478:conserved hypothetical protein (possible N6-adenine-specific methylase):mannose-specific phosphotransferase element:->->:332343..332631 289 taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 62 End: 172 ............................................................. M L K F P P H K I T F Q R Q D V V F N A I N S S Y L L I V I T I F E L S I ..................................................................................................................... Protein_Len: 36 Strand: + Start: 159 End: 266 .............................................................................................................................................................. M N S L F D D F H Q M N T L S H H A N V C D R F E F F A E T I Q T S N F ....................... Protein_Len: 32 Strand: + Start: 193 End: 288 ................................................................................................................................................................................................ M H L A I M Q T F A T V L N F S L K R F K R L I F N N L K E V L . Protein_Len: 31 Strand: - Start: 171 End: 263 .......................................................................................................................................................................... K V V K M L H I C K A M M C V N A V T K F K E S F R N L R R M .......................... Protein_Len: 34 Strand: - Start: 97 End: 198 ................................................................................................ R W S T T K L A I L E D Y K N I T I V I K S S E I Q R S E D F S Y M ........................................................................................... taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00475:AA00478|AA00475:AA00478:conserved hypothetical protein (possible N6-adenine-specific methylase):mannose-specific phosphotransferase element:->->:332343..332631 289 taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Intra-Species Hit: Count: 1 Min: 1 Max: 289 Len: 289 Subject: aact_AA00475_AA00478|conserved hypothetical protein (possible N6-adenine-specific methylase):mannose-specific phosphotransferase element|POSITIVE:POSITIVE|[332343,332631]|289 HSP 1 e-value: 1.0E-162 bit: 573.0 Len: 289 Query Start:1 Query End:289 Subject Strand: POSITIVE Subject Start: 1 Subject End: 289 taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga taaacgggtttaataggcgacgctgtaaacgcaacctctttcaccaagattttcataacaaattcttaaatttcccccacataaaataacatttcagcgccaagatgttgtttttaatgctatcaactcatcgtacttgttgatcgtgatcacgatttttgaactctctatttgacgactttcatcaaatgaatacacttagccatcatgcaaacgtttgcgaccgttttgaatttttcgctgaaacgattcaaacgtctaatttttaataacttaaaggaggttttga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACGGGUUUAAUAGGCGACGCUGUAAACGCAACCUCUUUCACCAAGAUUUUCAUAACAAAUUCUUAAAUUUCCCCCACAUAAAAUAACAUUUCAGCGCCAAGAUGUUGUUUUUAAUGCUAUCAACUCAUCGUACUUGUUGAUCGUGAUCACGAUUUUUGAACUCUCUAUUUGACGACUUUCAUCAAAUGAAUACACUUAGCCAUCAUGCAAACGUUUGCGACCGUUUUGAAUUUUUCGCUGAAACGAUUCAAACGUCUAAUUUUUAAUAACUUAAAGGAGGUUUUGA .....(.(((((...(((...))).))))).).((((((((....((((.(((......))).))))..........(((..((((((((((((........)))))))))))).......((((((...........)))))).))).....(((.((((((.((..(((((((.(.....).))))))).......(((((.......((((((........)).))))........)))))...)))))))).))).................))))))))..... (-48.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAAAACCUCCUUUAAGUUAUUAAAAAUUAGACGUUUGAAUCGUUUCAGCGAAAAAUUCAAAACGGUCGCAAACGUUUGCAUGAUGGCUAAGUGUAUUCAUUUGAUGAAAGUCGUCAAAUAGAGAGUUCAAAAAUCGUGAUCACGAUCAACAAGUACGAUGAGUUGAUAGCAUUAAAAACAACAUCUUGGCGCUGAAAUGUUAUUUUAUGUGGGGGAAAUUUAAGAAUUUGUUAUGAAAAUCUUGGUGAAAGAGGUUGCGUUUACAGCGUCGCCUAUUAAACCCGUUUA ......(((((.((((....)))).......((((..((..(((((((((.......((((((((........)))))...)))..(((((((((.(((((((((((.....)))))))).))).((((((..((((((...............))))))...))))..))..........))).)))))))))))))))...))..)))))))))......((((.(((......))).))))(((.....((((.((((.....)))).)))).....)))...... (-62.46) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 306 258 215 220 181 aact:AA02427|5end_hypothetical_protein_1674185..1674415_POSITIVE 292 260 214 172 124 aact:AA00540|5end_hypothetical_protein_370050..370280_POSITIVE 288 240 202 70 34 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 284 225 196 140 107 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 280 216 174 124 77 aact:AA01189|5end_conserved_hypothetical_protein_(possible_glycosyl_transferase)_814355..814585_POSITIVE 279 42 3 80 45 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 277 46 5 143 101 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 277 244 201 148 101 aact:AA01341|5end_acriflavine_resistance_protein_906884..907114_POSITIVE 276 249 207 70 20 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 273 149 110 208 169 aact:AA01914|5end_conserved_hypothetical_protein_1290126..1290356_POSITIVE 270 148 102 219 159 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 268 247 203 44 1 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 267 250 201 194 153 aact:AA02579|5end_aminotransferase_nifS_protein_1795773..1796003_POSITIVE 267 57 14 49 1 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 267 50 5 203 154 aact:AA00342|5end_hypothetical_protein_229695..229925_POSITIVE 266 259 215 219 181 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 261 248 205 215 172 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 261 37 5 217 180 aact:AA02899|5end_oligopeptide_ABC_transporter,_ATP-binding_protein_2030063..2030293_POSITIVE 260 226 181 81 39 aact:AA01932|5end_tRNA_pseudouridine_synthase_B_1305098..1305328_POSITIVE 259 216 173 65 10 aact:AA02250|5end_possible_cytochrome_c-type_protein_1530125..1530355_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 248 201 133 85 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 307 47 6 81 33 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 292 260 214 89 41 aact:AA00539|3end_ADP-L-glycero-D-manno-heptose-6-epimerase_370047..370197_POSITIVE 289 46 3 110 60 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 280 216 174 124 77 aact:AA01188|3end_conserved_hypothetical_protein_814435..814585_POSITIVE 277 46 5 140 98 aact:AA01759|3end_hydrogenase_isoenzymes_formation_protein_1189864..1190014_POSITIVE 270 148 102 148 88 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 268 241 201 60 24 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 261 248 205 118 75 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 260 246 201 138 82 aact:AA02052|3end_probable_exodeoxyribonuclease_VII_small_subunit_1398909..1399059_POSITIVE 260 226 181 78 36 aact:AA01930|3end_ribosome-binding_factor_A_1305175..1305325_POSITIVE 256 76 31 46 5 aact:AA01857|3end_glutamate-ammonia-ligase_adenylyltransferase_1250357..1250507_POSITIVE 256 248 202 151 98 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 252 139 91 141 102 aact:AA01790|3end_riboflavin_bifunctional_biosynthesis_protein_1208135..1208285_POSITIVE 249 162 122 113 77 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 249 40 3 72 26 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 249 114 77 128 93 aact:AA00415|3end_D-methionine_ABC_transporter,_ATP-binding_protein_282764..282914_POSITIVE 248 170 129 140 103 aact:AA00799|3end_iron(III)_dicitrate_transport_ATP-binding_protein_548502..548652_POSITIVE 247 242 205 122 88 aact:AA01794|3end_glycine_cleavage_system_transcription_activator_1210690..1210840_POSITIVE 247 248 209 124 84 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE