Origin IGS: ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggcttgtcactttttttatctgcctaatcaaccactttctttcccctgtgaatggaaaaaagcaaaatatttttgaaatatcaatgtattaaaatgtttcgaacaactgaggttgagaaacgcgcgccatcgcgagttatcaataacaagatttaaaaaaattaaggatgtaaaa Mask Tandem Repeat Region ================================================ ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Find is-nt database================================================ Query_seq: AA00473:AA00474|AA00473:AA00474:tryptophanyl-tRNA synthetase:cell division protein Y (GTP-binding signal recognition particle):->->:330138..330314 177 ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00473:AA00474|AA00473:AA00474:tryptophanyl-tRNA synthetase:cell division protein Y (GTP-binding signal recognition particle):->->:330138..330314 177 ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00473:AA00474|AA00473:AA00474:tryptophanyl-tRNA synthetase:cell division protein Y (GTP-binding signal recognition particle):->->:330138..330314 177 ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 14 End: 145 ............. M F L N L V I D N S R W R A F L N L S C S K H F N T L I F Q K Y F A F F H S Q G K E S G ................................ Protein_Len: 34 Strand: - Start: 23 End: 124 ...................... I K N N I V R S P A R K E V E T T R F M K I C Q Y K L F I K S K E M ..................................................... Protein_Len: 32 Strand: - Start: 1 End: 96 K V D K I K K F R T I S L E R H R A N R L R L Q E F C K L V N M ................................................................................. ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00473:AA00474|AA00473:AA00474:tryptophanyl-tRNA synthetase:cell division protein Y (GTP-binding signal recognition particle):->->:330138..330314 177 ttttacatccttaatttttttaaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagataaaaaaagtgacaagccta Intra-Species Hit: Count: 1 Min: 22 Max: 158 Len: 137 Subject: aact_AA00473_AA00474|tryptophanyl-tRNA synthetase:cell division protein Y (GTP-binding signal recognition particle)|POSITIVE:POSITIVE|[330138,330314]|177 HSP 1 e-value: 4.0E-72 bit: 272.0 Len: 137 Query Start:22 Query End:158 Subject Strand: POSITIVE Subject Start: 22 Subject End: 158 .....................aaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagat................... .....................aaatcttgttattgataactcgcgatggcgcgcgtttctcaacctcagttgttcgaaacattttaatacattgatatttcaaaaatattttgcttttttccattcacaggggaaagaaagtggttgattaggcagat................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUCUUGUUAUUGAUAACUCGCGAUGGCGCGCGUUUCUCAACCUCAGUUGUUCGAAACAUUUUAAUACAUUGAUAUUUCAAAAAUAUUUUGCUUUUUUCCAUUCACAGGGGAAAGAAAGUGGUUGAUUAGGCAGAU ..((((.(((((..((....((((....)))).(((((.((((....))))...)))))..........))..)))..((((...((((((.(((((..(........)..))))))))))).))))....)))))) (-26.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCUGCCUAAUCAACCACUUUCUUUCCCCUGUGAAUGGAAAAAAGCAAAAUAUUUUUGAAAUAUCAAUGUAUUAAAAUGUUUCGAACAACUGAGGUUGAGAAACGCGCGCCAUCGCGAGUUAUCAAUAACAAGAUUU ((((......((((((.((((.(((((.........))))))))).........((((((((((............)))))))))).......))))))..(((.((((....)))).)))..........)))).. (-25.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 592 70 21 195 142 aact:AA00729|5end_possible_hemolysin_(TlyC-like)_500204..500434_POSITIVE 561 59 19 144 104 aact:AA02453|5end_conserved_hypothetical_protein_1688947..1689177_POSITIVE 389 100 51 182 134 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 336 59 21 173 132 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 317 60 19 176 125 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 313 60 17 169 120 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 308 54 15 210 162 aact:AA01441|5end_tRNA/rRNA_methylase_966490..966720_POSITIVE 307 34 1 38 3 aact:AA01637|5end_ribonucleoside_diphosphate_reductase_1106657..1106887_POSITIVE 306 47 3 212 174 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 298 48 5 189 155 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 298 48 5 51 17 aact:AA01773|5end_hypothetical_protein_1194877..1195107_POSITIVE 283 63 21 93 55 aact:AA01325|5end_formamidopyrimidine-DNA_glycosylase_894747..894977_POSITIVE 280 56 23 82 40 aact:AA02964|5end_opacity_associated_protein_A_2072738..2072968_POSITIVE 280 44 1 228 166 aact:AA01634|5end_hypothetical_protein_1104324..1104554_POSITIVE 277 54 20 178 141 aact:AA02045|5end_PTS_system,_galactitol-specific_IIB_component_1391956..1392186_POSITIVE 277 54 13 64 13 aact:AA01889|5end_conserved_hypothetical_protein_(possible_permease)_1273182..1273412_POSITIVE 277 55 13 216 171 aact:AA01018|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_711663..711893_POSITIVE 276 38 20 112 95 aact:AA02742|5end_tRNA_nucleotidyltransferase_1921308..1921538_POSITIVE 275 43 24 201 182 aact:AA02573|5end_ABC_transporter,_ATP-binding_protein_1790409..1790639_POSITIVE 275 39 10 130 104 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 561 59 19 43 3 aact:AA02451|3end_conserved_hypothetical_protein_1688926..1689076_POSITIVE 313 60 17 56 7 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 308 54 15 138 90 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 306 47 3 72 34 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 301 44 11 79 43 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 298 48 5 36 2 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 280 61 22 143 101 aact:AA01296|3end_shikimate_kinase_(shikimic_acid_kinase_I)_878952..879102_POSITIVE 277 54 13 88 37 aact:AA01888|3end_hypothetical_protein_1273286..1273436_POSITIVE 276 38 20 118 101 aact:AA02740|3end_conserved_hypothetical_protein_1921394..1921544_POSITIVE 275 39 10 127 101 aact:AA01759|3end_hydrogenase_isoenzymes_formation_protein_1189864..1190014_POSITIVE 273 60 14 73 27 aact:AA00085|3end_menaquinone-specific_isochorismate;_isochorismate_mutase_63523..63673_POSITIVE 269 57 12 46 2 aact:AA00889|3end_conserved_hypothetical_protein_617110..617260_POSITIVE 268 52 20 91 70 aact:AA00886|3end_hypothetical_protein_616855..617005_POSITIVE 265 40 5 119 76 aact:AA00738|3end_hypothetical_protein_505256..505406_POSITIVE 261 49 1 112 73 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE 260 51 17 128 83 aact:AA01853|3end_hypothetical_protein_1246645..1246795_POSITIVE 260 60 22 85 43 aact:AA01192|3end_conserved_hypothetical_protein_(possible_membrane_protein)_817977..818127_POSITIVE 259 65 22 69 22 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 259 56 19 80 46 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 258 50 1 64 5 aact:AA02563|3end_transketolase_C-terminal_section_1786260..1786410_POSITIVE