Origin IGS: ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatttgttaaaaaacactgtgttcattttcaatttaccataaatgagtacatttgttttgtccccaaaatttatgtttttttagagaaattaaccttttatgcaataggtaatacgtctggaccgcgctatggtacattattgttcgtgtggttgaccttatgagcgttgttttagcag Mask Tandem Repeat Region ================================================ ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Find is-nt database================================================ Query_seq: AA00464:AA00465|AA00464:AA00465:conserved hypothetical protein:hypothetical protein:->->:324166..324345 180 ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00464:AA00465|AA00464:AA00465:conserved hypothetical protein:hypothetical protein:->->:324166..324345 180 ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00464:AA00465|AA00464:AA00465:conserved hypothetical protein:hypothetical protein:->->:324166..324345 180 ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 18 End: 143 ................. M R S T T R T I M Y H S A V Q T Y Y L L H K R L I S L K K H K F W G Q N K C T H L W ..................................... Protein_Len: 41 Strand: - Start: 19 End: 141 .................. L T L W V F L L T G Y R P G S T N G I A Y F T L K E L F V Y I K P V F C I Y E N M ....................................... ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00464:AA00465|AA00464:AA00465:conserved hypothetical protein:hypothetical protein:->->:324166..324345 180 ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 180 Len: 180 Subject: aact_AA00464_AA00465|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[324166,324345]|180 HSP 1 e-value: 3.0E-85 bit: 315.0 Len: 180 Query Start:1 Query End:180 Subject Strand: POSITIVE Subject Start: 1 Subject End: 180 ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctnnnnnnncataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta ctgctaaaacaacgctcataaggtcaaccacacgaacaataatgtaccatagcgcggtccagacgtattacctattgcataaaaggttaatttctctaaaaaaacataaattttggggacaaaacaaatgtactcatttatggtaaattgaaaatgaacacagtgttttttaacaaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGCUAAAACAACGCUCAUAAGGUCAACCACACGAACAAUAAUGUACCAUAGCGCGGUCCAGACGUAUUACCUAUUGCAUAAAAGGUUAAUUUCUCUAAAAAAACAUAAAUUUUGGGGACAAAACAAAUGUACUCAUUUAUGGUAAAUUGAAAAUGAACACAGUGUUUUUUAACAAAUUA .((.(((((.((((((.....((....)).......((((....((((((((........(((...(((((((..........))).))))...)))......((((...(((((....)))))...))))......)))))))).))))...........))))))))))).))..... (-28.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUGUUAAAAAACACUGUGUUCAUUUUCAAUUUACCAUAAAUGAGUACAUUUGUUUUGUCCCCAAAAUUUAUGUUUUUUUAGAGAAAUUAACCUUUUAUGCAAUAGGUAAUACGUCUGGACCGCGCUAUGGUACAUUAUUGUUCGUGUGGUUGACCUUAUGAGCGUUGUUUUAGCAG .....(((((((....((..(((((((.......(((((......(((.((((..((((((....))))))..)))).)))..((((........)))).........)))))...(((..((((((((.(..(((...)))..)..)))))))))))...)))))))..))))))))). (-35.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 315 65 24 78 37 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 283 59 13 47 7 aact:AA01238|5end_50S_ribosomal_protein_L36_844248..844478_POSITIVE 277 38 1 209 175 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 270 86 41 49 5 aact:AA02552|5end_iron_(chelated)_ABC_transporter,_permease_protein_1778495..1778725_POSITIVE 269 88 43 121 63 aact:AA02747|5end_ribose-phosphate_pyrophosphokinase;_phosphoribosylpyrophosphate_synthase_1924324..1924554_POSITIVE 269 79 31 212 168 aact:AA01807|5end_electron_transport_complex_protein_1216696..1216926_POSITIVE 269 58 13 210 163 aact:AA01757|5end_cobalt_transport_ATP-binding_protein_1187137..1187367_POSITIVE 265 87 43 215 158 aact:AA01750|5end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1183750..1183980_POSITIVE 263 60 11 168 122 aact:AA02742|5end_tRNA_nucleotidyltransferase_1921308..1921538_POSITIVE 263 57 14 226 185 aact:AA01708|5end_glutaredoxin_1158136..1158366_POSITIVE 263 58 13 59 16 aact:AA00807|5end_fumarate_reductase_13_kD_hydrophobic_protein_552767..552997_POSITIVE 258 63 21 64 18 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 257 71 37 198 163 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 251 65 21 52 4 aact:AA01843|5end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1238377..1238607_POSITIVE 250 58 11 169 112 aact:AA01941|5end_hypothetical_protein_1312480..1312710_POSITIVE 250 57 17 165 123 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 246 77 37 69 21 aact:AA01688|5end_conserved_hypothetical_protein;_possible_membrane_protein_1139635..1139865_POSITIVE 246 79 32 226 172 aact:AA01202|5end_integrase/recombinase_823240..823470_POSITIVE 244 90 42 185 134 aact:AA02928|5end_conserved_hypothetical_protein_2048361..2048591_POSITIVE 244 90 42 57 6 aact:AA02927|5end_hypothetical_protein_2048233..2048463_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 274 56 13 147 117 aact:AA01238|3end_50S_ribosomal_protein_L36_844438..844588_POSITIVE 270 86 41 49 5 aact:AA02551|3end_iron_(chelated)_ABC_transporter,_permease_protein_1778575..1778725_POSITIVE 263 58 13 46 3 aact:AA00806|3end_fumarate_reductase_15_kD_hydrophobic_protein_552834..552984_POSITIVE 258 63 21 52 6 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 255 64 22 65 12 aact:AA01839|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_1237291..1237441_POSITIVE 251 65 21 62 14 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 250 58 11 149 92 aact:AA01939|3end_NAD(P)_transhydrogenase_subunit_beta_1312540..1312690_POSITIVE 244 90 42 149 98 aact:AA02927|3end_hypothetical_protein_2048405..2048555_POSITIVE 244 90 42 91 40 aact:AA02926|3end_hypothetical_protein_2048347..2048497_POSITIVE 243 58 13 106 63 aact:AA01626|3end_N_utilization_substance_protein_B_1100946..1101096_POSITIVE 241 57 13 134 85 aact:AA00087|3end_possible_hydrolase_(alpha/beta_fold_family)_66022..66172_POSITIVE 240 88 47 124 87 aact:AA00777|3end_soluble_lytic_murein_transglycosylase_536612..536762_POSITIVE 235 60 13 118 75 aact:AA02315|3end_conserved_hypothetical_protein_1581752..1581902_POSITIVE 234 57 11 149 101 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 231 87 42 96 44 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 229 106 61 115 76 aact:AA00568|3end_hypothetical_protein_391276..391426_POSITIVE 228 24 2 76 54 aact:AA00991|3end_conserved_hypothetical_protein_693741..693891_POSITIVE 227 78 38 120 63 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 227 78 33 78 41 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 226 97 51 53 1 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE