Origin IGS: catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtaactcctgagtgcgttgttaattgacggtagttatcgtacctgttctattccattgtgcaatttaagcaatcgtttgctatgtaaaattctgatcatggtcacactttgtcgggataattggcggagaaataatcagtgatcttttctcttgaattctgtgaattcaccacaatatagcccacatcttttcttagtaaaaaaggacaaatg Mask Tandem Repeat Region ================================================ catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Find is-nt database================================================ Query_seq: AA00461:AA00463|AA00461:AA00463:heat shock protein; protease:class B acid phosphatase:->->:323040..323253 214 catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00461:AA00463|AA00461:AA00463:heat shock protein; protease:class B acid phosphatase:->->:323040..323253 214 catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00461:AA00463|AA00461:AA00463:heat shock protein; protease:class B acid phosphatase:->->:323040..323253 214 catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 2 End: 109 . M C P F L L R K D V G Y I V V N S Q N S R E K I T D Y F S A N Y P D K V ......................................................................................................... Protein_Len: 44 Strand: - Start: 75 End: 206 .......................................................................... Q N N R R W N D R C L T V M I L I K C L L R N S L N C L P I S C T R Y S G D I L L A S M ........ catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00461:AA00463|AA00461:AA00463:heat shock protein; protease:class B acid phosphatase:->->:323040..323253 214 catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Intra-Species Hit: Count: 1 Min: 1 Max: 214 Len: 214 Subject: aact_AA00461_AA00463|heat shock protein; protease:class B acid phosphatase|POSITIVE:POSITIVE|[323040,323253]|214 HSP 1 e-value: 1.0E-118 bit: 424.0 Len: 214 Query Start:1 Query End:214 Subject Strand: POSITIVE Subject Start: 1 Subject End: 214 catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc catttgtccttttttactaagaaaagatgtgggctatattgtggtgaattcacagaattcaagagaaaagatcactgattatttctccgccaattatcccgacaaagtgtgaccatgatcagaattttacatagcaaacgattgcttaaattgcacaatggaatagaacaggtacgataactaccgtcaattaacaacgcactcaggagttacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUUUGUCCUUUUUUACUAAGAAAAGAUGUGGGCUAUAUUGUGGUGAAUUCACAGAAUUCAAGAGAAAAGAUCACUGAUUAUUUCUCCGCCAAUUAUCCCGACAAAGUGUGACCAUGAUCAGAAUUUUACAUAGCAAACGAUUGCUUAAAUUGCACAAUGGAAUAGAACAGGUACGAUAACUACCGUCAAUUAACAACGCACUCAGGAGUUACC ......((((.................((((((..(((...((((((((((...))))))..((((((.((((...)))).)))))).))))..)))))).))).((((((.((((......(((((.....(((((....))))).))))).....))))....((...((((.......)))).)).........)))))).))))...... (-42.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUAACUCCUGAGUGCGUUGUUAAUUGACGGUAGUUAUCGUACCUGUUCUAUUCCAUUGUGCAAUUUAAGCAAUCGUUUGCUAUGUAAAAUUCUGAUCAUGGUCACACUUUGUCGGGAUAAUUGGCGGAGAAAUAAUCAGUGAUCUUUUCUCUUGAAUUCUGUGAAUUCACCACAAUAUAGCCCACAUCUUUUCUUAGUAAAAAAGGACAAAUG ((((((((((.(((.........))).).)).))))))).....((((((.........(((.......)))...((..(((((((...(((((((.((.((.....)).)))))))))...(((.(((((((..(((...)))..))))))).((((((...)))))).)))..)))))))..))..................)))))).... (-43.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 46 3 177 136 aact:AA00419|5end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284337..284567_POSITIVE 302 140 96 65 21 aact:AA02940|5end_conserved_hypothetical_protein_2057922..2058152_POSITIVE 284 110 63 206 157 aact:AA02087|5end_1,4-alpha-glucan_branching_enzyme_1418934..1419164_POSITIVE 280 119 71 70 33 aact:AA01863|5end_hypothetical_protein_1252011..1252241_POSITIVE 271 120 73 152 91 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 265 112 74 43 7 aact:AA00093|5end_L-serine_dehydratase_(L-serine_deaminase)_69732..69962_POSITIVE 260 119 74 133 83 aact:AA02109|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436563..1436793_POSITIVE 257 118 72 94 49 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 256 140 91 215 175 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 254 209 166 82 30 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 254 130 81 227 178 aact:AA01450|5end_hypothetical_protein_971860..972090_POSITIVE 254 200 153 220 167 aact:AA00327|5end_conserved_hypothetical_protein_222339..222569_POSITIVE 250 130 86 192 143 aact:AA01623|5end_alanine_racemase_1_1097077..1097307_POSITIVE 250 121 82 38 7 aact:AA00311|5end_L-fuculose-phosphate_aldolase_211434..211664_POSITIVE 248 77 40 146 100 aact:AA01737|5end_exoribonuclease_II_1172647..1172877_POSITIVE 244 117 88 51 20 aact:AA01386|5end_30S_ribosomal_protein_S1_935974..936204_POSITIVE 244 120 76 102 31 aact:AA01195|5end_acyl_carrier_protein_819849..820079_POSITIVE 243 130 81 120 68 aact:AA00895|5end_hypothetical_protein_621285..621515_POSITIVE 243 114 73 53 8 aact:AA00475|5end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_331621..331851_POSITIVE 242 124 88 75 45 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 46 3 68 27 aact:AA00417|3end_outer_membrane_lipoprotein_precursor_(probable_D-methionine-binding_lipoprotein)_284308..284458_POSITIVE 280 119 71 86 49 aact:AA01861|3end_phosphomethylpyrimidine_kinase_1252107..1252257_POSITIVE 272 177 133 144 104 aact:AA02296|3end_conserved_hypothetical_protein_(possible_cell_wall-associated_hydrolase)_1566581..1566731_POSITIVE 272 177 133 144 104 aact:AA00620|3end_hydrolase_425693..425843_POSITIVE 260 119 74 133 83 aact:AA02108|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436643..1436793_POSITIVE 250 121 82 38 7 aact:AA00310|3end_conserved_hypothetical_protein_211514..211664_POSITIVE 248 77 40 133 87 aact:AA01735|3end_hypothetical_protein_1172714..1172864_POSITIVE 244 117 88 32 1 aact:AA01385|3end_50S_ribosomal_protein_L9_936035..936185_POSITIVE 244 120 76 96 25 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 243 130 81 105 53 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 243 114 73 76 31 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 242 124 88 75 45 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 238 112 71 57 13 aact:AA00691|3end_conserved_hypothetical_protein_(possible_multidrug/sugar_efflux_transporter)_473155..473305_POSITIVE 237 47 2 73 33 aact:AA00277|3end_biotin_synthesis_protein_194690..194840_POSITIVE 235 67 25 122 76 aact:AA01853|3end_hypothetical_protein_1246645..1246795_POSITIVE 234 189 143 115 56 aact:AA02939|3end_phosphoserine_phosphatase_2057902..2058052_POSITIVE 234 111 71 53 9 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 233 46 24 108 86 aact:AA02317|3end_conserved_hypothetical_protein_1583887..1584037_POSITIVE 233 177 133 121 81 aact:AA00411|3end_hydrolase_276288..276438_POSITIVE 233 120 71 150 104 aact:AA00377|3end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_254300..254450_POSITIVE