Origin IGS: actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aataagtctcctaagattaattgaaaaaactgcgtcagttatagcaaaaaaacgctttttttgaaaggaaaaacgccgaaaatttttaccgcactttaggcgtcaaaagagtgattaatttgatttttttatcggattttgacgtaataaaatttgtatgaaaaccatgtaatcgattgccttacgtcgaatctgtgaaatagatcaaagtgtcgcaaattaatcggaaataatcattttacttttcgtggtttttcgataggctaggggaagatttgtacattgactttagggaaggagagagt Mask Tandem Repeat Region ================================================ actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Find is-nt database================================================ Query_seq: AA00452:AA00453|AA00452:AA00453:phosphomannomutase; phosphoglucomutase:phosphoglyceromutase:->->:317084..317388 305 actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00452:AA00453|AA00452:AA00453:phosphomannomutase; phosphoglucomutase:phosphoglyceromutase:->->:317084..317388 305 actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00452:AA00453|AA00452:AA00453:phosphomannomutase; phosphoglucomutase:phosphoglyceromutase:->->:317084..317388 305 actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 82 End: 174 ................................................................................. M N L R H F D L F H R F D V R Q S I T W F S Y K F Y Y V K I R ................................................................................................................................... actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 239 End: 268 ..............................................................................................................................................................................................................................................CTTTCAAAAAAAGCGTTTTTTTGCTATAAC..................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00452:AA00453|AA00452:AA00453:phosphomannomutase; phosphoglucomutase:phosphoglyceromutase:->->:317084..317388 305 actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Intra-Species Hit: Count: 1 Min: 1 Max: 305 Len: 305 Subject: aact_AA00452_AA00453|phosphomannomutase; phosphoglucomutase:phosphoglyceromutase|POSITIVE:POSITIV E|[317084,317388]|305 HSP 1 e-value: 1.0E-134 bit: 480.0 Len: 305 Query Start:1 Query End:305 Subject Strand: POSITIVE Subject Start: 1 Subject End: 305 actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgatnnnnnnntcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcnnnnnnngcgnnnnnnngctataactgacgcagttttttcaattaatcttaggagacttatt actctctccttccctaaagtcaatgtacaaatcttcccctagcctatcgaaaaaccacgaaaagtaaaatgattatttccgattaatttgcgacactttgatctatttcacagattcgacgtaaggcaatcgattacatggttttcatacaaattttattacgtcaaaatccgataaaaaaatcaaattaatcactcttttgacgcctaaagtgcggtaaaaattttcggcgtttttcctttcaaaaaaagcgtttttttgctataactgacgcagttttttcaattaatcttaggagacttatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUCUCUCCUUCCCUAAAGUCAAUGUACAAAUCUUCCCCUAGCCUAUCGAAAAACCACGAAAAGUAAAAUGAUUAUUUCCGAUUAAUUUGCGACACUUUGAUCUAUUUCACAGAUUCGACGUAAGGCAAUCGAUUACAUGGUUUUCAUACAAAUUUUAUUACGUCAAAAUCCGAUAAAAAAAUCAAAUUAAUCACUCUUUUGACGCCUAAAGUGCGGUAAAAAUUUUCGGCGUUUUUCCUUUCAAAAAAAGCGUUUUUUUGCUAUAACUGACGCAGUUUUUUCAAUUAAUCUUAGGAGACUUAUU ....((((((......................................(((((((..((((((.................((((((((((.....((.(((......))).))....(((((((((.(((((......))))).))............)))))))..................))))))))))....(((((.((((....).))).))))).))))))..))))))).....(((((..(((((..((......))..)))))..)))))............))))))...... (-49.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAGUCUCCUAAGAUUAAUUGAAAAAACUGCGUCAGUUAUAGCAAAAAAACGCUUUUUUUGAAAGGAAAAACGCCGAAAAUUUUUACCGCACUUUAGGCGUCAAAAGAGUGAUUAAUUUGAUUUUUUUAUCGGAUUUUGACGUAAUAAAAUUUGUAUGAAAACCAUGUAAUCGAUUGCCUUACGUCGAAUCUGUGAAAUAGAUCAAAGUGUCGCAAAUUAAUCGGAAAUAAUCAUUUUACUUUUCGUGGUUUUUCGAUAGGCUAGGGGAAGAUUUGUACAUUGACUUUAGGGAAGGAGAGAGU ......((((((........((....)).....((((((....(((((....((.(((((((....)))))))))((((...(((((..(((.(....))))..))))).(((((...(((((((((((((.(((((((.(((((((...((((((((((.....))))))...))))...))))))))))))))))))).))))))))..))))).......))))..............(((..(.((((((......)))))).)..))).)))))..))))))........)))))).... (-70.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 403 243 204 133 88 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 387 246 204 119 73 aact:AA02662|5end_hypothetical_protein_1853283..1853513_POSITIVE 373 238 194 178 130 aact:AA02177|5end_conserved_hypothetical_protein_1488963..1489193_POSITIVE 365 237 191 168 123 aact:AA01298|5end_competence_protein_D_880554..880784_POSITIVE 358 240 193 167 117 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 357 244 205 90 53 aact:AA01753|5end_conserved_hypothetical_protein_1185322..1185552_POSITIVE 353 238 193 213 171 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 353 238 193 83 41 aact:AA02475|5end_hypothetical_protein_1711480..1711710_POSITIVE 352 253 211 76 25 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 348 238 191 164 112 aact:AA01308|5end_cyclic_AMP_receptor_protein_888044..888274_POSITIVE 346 238 195 216 173 aact:AA02053|5end_acetyl-CoA_carboxylase_carboxyltransferase,_beta_1398928..1399158_POSITIVE 346 239 191 174 117 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 338 256 211 61 8 aact:AA00313|5end_hypothetical_protein_212822..213052_POSITIVE 336 239 191 74 15 aact:AA02239|5end_molybdopterin_biosynthesis_protein_D_1523216..1523446_POSITIVE 332 237 195 184 129 aact:AA02915|5end_thioredoxin_m_2040652..2040882_POSITIVE 331 238 201 166 129 aact:AA02762|5end_ribonuclease_H_1933164..1933394_POSITIVE 330 240 194 76 25 aact:AA01538|5end_conserved_hypothetical_protein_1031527..1031757_POSITIVE 327 249 202 150 98 aact:AA02215|5end_conserved_hypothetical_protein_1508800..1509030_POSITIVE 323 239 192 151 104 aact:AA00194|5end_oxygen-independent_coproporphyrinogen_III_oxidase_135545..135775_POSITIVE 321 246 201 159 109 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 387 246 204 103 57 aact:AA02659|3end_conserved_hypothetical_protein_1853347..1853497_POSITIVE 373 238 194 101 53 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 365 237 191 145 100 aact:AA01297|3end_competence_protein_E_precursor_880611..880761_POSITIVE 358 240 193 83 33 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 357 244 205 80 43 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 353 238 193 71 29 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 350 236 193 117 71 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 350 236 193 133 87 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE 346 238 195 117 74 aact:AA02052|3end_probable_exodeoxyribonuclease_VII_small_subunit_1398909..1399059_POSITIVE 346 239 191 134 77 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 338 256 211 81 28 aact:AA00312|3end_conserved_hypothetical_protein_(possible_hydroxypyruvate_isomerase)_212922..213072_POSITIVE 336 239 191 70 11 aact:AA02237|3end_molybdopterin_biosynthesis_protein_E_chain_1523292..1523442_POSITIVE 331 238 201 72 35 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 330 240 194 76 25 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 328 240 199 92 50 aact:AA02172|3end_conserved_hypothetical_protein_1486650..1486800_POSITIVE 323 239 192 71 24 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 321 246 201 142 92 aact:AA02824|3end_conserved_hypothetical_protein_1986399..1986549_POSITIVE 315 235 193 101 55 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 311 237 202 107 72 aact:AA02926|3end_hypothetical_protein_2048347..2048497_POSITIVE 309 218 171 56 2 aact:AA01600|3end_phosphotransferase_system_enzyme_I_1082285..1082435_POSITIVE