Origin IGS: tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagagtttttgacttgaaaaatatcatttttgcgatctgaatcacaaaattacttcctaaacctatatagagagaaaattaccgataagctaatctctattattagcacataaatagaggtttattatgcaaaaattacagttatcgcttaaagaacagttcagattatggcttgaaacagagcaggaaatagatgaaatactagctatagtaaagataccttgtattttatctaagttagatagtaacttggctgttgtgtgtgggaataggctagaaaaacgttacttatataaatggattaagtattttcgtatagaccgtgtaattggaaacgttttttaagatctgctaaattattatattattatttttaatttctaattgaaaaggctttttcaaggttgtttaaatttttagtttctgttttagaatgaaataagtgactttattttgatagggaactctcgttccctacttcta Mask Tandem Repeat Region ================================================ tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Find is-nt database================================================ Query_seq: AA00440:AA00442|AA00440:AA00442:hypothetical protein:conserved hypothetical protein (possible zinc finger protein):->->:305846..306328 483 tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00440:AA00442|AA00440:AA00442:hypothetical protein:conserved hypothetical protein (possible zinc finger protein):->->:305846..306328 483 tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00440:AA00442|AA00440:AA00442:hypothetical protein:conserved hypothetical protein (possible zinc finger protein):->->:305846..306328 483 tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 257 End: 391 ................................................................................................................................................................................................................................................................ M Q G I F T I A S I S S I S C S V S S H N L N C S L S D N C N F C I I N L Y L C A N N R D ............................................................................................ Protein_Len: 30 Strand: - Start: 374 End: 463 ..................................................................................................................................................................................................................................................................................................................................................................................... T S I I S I L K D T I K E R Y L N L F Y N Q S E S R L F S M .................... Protein_Len: 60 Strand: - Start: 142 End: 360 ............................................................................................................................................. I W K Y L Y R K E L R N G C V V A L N S D L K S L I C P I K V I A L I E D I E Q E T E L W L R F Q E K L S L Q L K Q M M ........................................................................................................................... tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 50 HP_score: -4.8 Tail_Score: -2.61997 Start: 231 End: 251 Full_Region: taccttgtattttat ctaagtta gatag taacttgg ctgttgtgtgtggga ......................................................................................................................................................................................................................................ctaagttagatagtaacttgg........................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 43 HP_score: -3.5 Tail_Score: -2.55544 Start: 251 End: 291 Full_Region: acagagcaggaaata gatgaaatactagctat agtaaag ataccttgtattttatc taagttagatagtaa ..........................................................................................................................................................................................................................................................gatgaaatactagctatagtaaagataccttgtattttatc................................................................................................................................................................................................ TransTerm Strand: + Conf: 47 HP_score: -3.2 Tail_Score: -3.42124 Start: 377 End: 395 Full_Region: AACCTCTATTTATGT GCTAAT AATAGAG ATTAGC TTATCGGTAATTTTC ........................................................................................................................................................................................................................................................................................................................................................................................GCTAATAATAGAGATTAGC........................................................................................ Find igs database================================================ Query_seq: AA00440:AA00442|AA00440:AA00442:hypothetical protein:conserved hypothetical protein (possible zinc finger protein):->->:305846..306328 483 tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattctaaaacagaaactaaaaatttaaacaaccttgaaaaagccttttcaattagaaattaaaaataataatataataatttagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta Intra-Species Hit: Count: 1 Min: 1 Max: 483 Len: 483 Subject: aact_AA00440_AA00442|hypothetical protein:conserved hypothetical protein (possible zinc finger protein)|POSITIVE:POSITIVE|[305846,306328]|483 HSP 1 e-value: 0.0 bit: 702.0 Len: 354 Query Start:130 Query End:483 Subject Strand: POSITIVE Subject Start: 130 Subject End: 483 .................................................................................................................................tagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta .................................................................................................................................tagcagatcttaaaaaacgtttccaattacacggtctatacgaaaatacttaatccatttatataagtaacgtttttctagcctattcccacacacaacagccaagttactatctaacttagataaaatacaaggtatctttactatagctagtatttcatctatttcctgctctgtttcaagccataatctgaactgttctttaagcgataactgtaatttttgcataataaacctctatttatgtgctaataatagagattagcttatcggtaattttctctctatataggtttaggaagtaattttgtgattcagatcgcaaaaatgatatttttcaagtcaaaaactcta HSP 2 e-value: 6.0E-21 bit: 103.0 Len: 52 Query Start:1 Query End:52 Subject Strand: POSITIVE Subject Start: 1 Subject End: 52 tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattc............................................................................................................................................................................................................................................................................................................................................................................................................................................... tagaagtagggaacgagagttccctatcaaaataaagtcacttatttcattc............................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAGUAGGGAACGAGAGUUCCCUAUCAAAAUAAAGUCACUUAUUUCAUUCUAAAACAGAAACUAAAAAUUUAAACAACCUUGAAAAAGCCUUUUCAAUUAGAAAUUAAAAAUAAUAAUAUAAUAAUUUAGCAGAUCUUAAAAAACGUUUCCAAUUACACGGUCUAUACGAAAAUACUUAAUCCAUUUAUAUAAGUAACGUUUUUCUAGCCUAUUCCCACACACAACAGCCAAGUUACUAUCUAACUUAGAUAAAAUACAAGGUAUCUUUACUAUAGCUAGUAUUUCAUCUAUUUCCUGCUCUGUUUCAAGCCAUAAUCUGAACUGUUCUUUAAGCGAUAACUGUAAUUUUUGCAUAAUAAACCUCUAUUUAUGUGCUAAUAAUAGAGAUUAGCUUAUCGGUAAUUUUCUCUCUAUAUAGGUUUAGGAAGUAAUUUUGUGAUUCAGAUCGCAAAAAUGAUAUUUUUCAAGUCAAAAACUCUA ((((((((((((((....)))))))))..((((((......))))))..)))))..........................(((((((((....((((.....)))).........................((.(((((.......(((..........))).....(((((((.((((((...........))))))....(((((((((((((..............(((((((((..........(((((.((((((.((.(((.......))).)).)))))).))))).......(((((((...((((((((...((...(((..(((.((((............)))).)))..))).))...))))).)))...)))))))..))))))...)))..............))))).))))))))...)))))))....)))))))..........)))))))))............ (-85.49) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAGUUUUUGACUUGAAAAAUAUCAUUUUUGCGAUCUGAAUCACAAAAUUACUUCCUAAACCUAUAUAGAGAGAAAAUUACCGAUAAGCUAAUCUCUAUUAUUAGCACAUAAAUAGAGGUUUAUUAUGCAAAAAUUACAGUUAUCGCUUAAAGAACAGUUCAGAUUAUGGCUUGAAACAGAGCAGGAAAUAGAUGAAAUACUAGCUAUAGUAAAGAUACCUUGUAUUUUAUCUAAGUUAGAUAGUAACUUGGCUGUUGUGUGUGGGAAUAGGCUAGAAAAACGUUACUUAUAUAAAUGGAUUAAGUAUUUUCGUAUAGACCGUGUAAUUGGAAACGUUUUUUAAGAUCUGCUAAAUUAUUAUAUUAUUAUUUUUAAUUUCUAAUUGAAAAGGCUUUUUCAAGGUUGUUUAAAUUUUUAGUUUCUGUUUUAGAAUGAAAUAAGUGACUUUAUUUUGAUAGGGAACUCUCGUUCCCUACUUCUA (((((((.....(((((((((....(((((((((...((((((.........((..(((........)))..))..............((((((.......))))))...........))))))...)))))))))..((((((...((((((((((..(((.((((((..(((...(((.(((((....((((((((((((.((.(((.......))).)).)))))))))))).((((.....))))....))))).)))..)))....(((((.....(((.((((((...........))))))....))).))).))..)))))).)))..)))))))))).......((((((..(((....)))...)))))).)))))).......))))))))).((((((....((((((........))))))..))))))..)))))))......((((((((....))))))))...... (-97.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 281 360 312 94 53 aact:AA02246|5end_peptidase_E_1527574..1527804_POSITIVE 276 338 296 228 191 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 272 380 332 218 170 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 269 316 278 204 172 aact:AA00813|5end_hypothetical_protein_558807..559037_POSITIVE 266 338 291 79 16 aact:AA02035|5end_translation_initiation_factor_IF-3_1386349..1386579_POSITIVE 264 249 210 88 39 aact:AA02940|5end_conserved_hypothetical_protein_2057922..2058152_POSITIVE 254 335 291 208 164 aact:AA01721|5end_hypothetical_protein_1163312..1163542_POSITIVE 254 335 291 174 130 aact:AA01720|5end_conserved_hypothetical_protein_1163278..1163508_POSITIVE 254 330 281 103 63 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 253 242 204 195 153 aact:AA02266|5end_exodeoxyribonuclease_V_beta_chain_1541680..1541910_POSITIVE 253 331 298 42 12 aact:AA01679|5end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1134356..1134586_POSITIVE 249 329 282 100 56 aact:AA00313|5end_hypothetical_protein_212822..213052_POSITIVE 247 39 4 228 191 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 246 320 279 145 101 aact:AA01963|5end_dnaK_suppressor_protein_1328924..1329154_POSITIVE 236 376 335 168 126 aact:AA00861|5end_transcription_repair_coupling_factor_592552..592782_POSITIVE 233 233 199 229 196 aact:AA02947|5end_riboflavin_biosynthesis_protein_2060871..2061101_POSITIVE 233 307 263 224 180 aact:AA02305|5end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1574778..1575008_POSITIVE 232 402 364 122 86 aact:AA02078|5end_hypothetical_protein_1412612..1412842_POSITIVE 232 358 311 92 56 aact:AA01642|5end_possible_iron(III)_ABC_transporter_1110970..1111200_POSITIVE 230 348 304 119 75 aact:AA01919|5end_conserved_hypothetical_protein_1292719..1292949_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 281 360 312 61 20 aact:AA02245|3end_hypothetical_protein_1527621..1527771_POSITIVE 276 338 296 133 96 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 272 380 332 127 79 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 254 335 291 132 88 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 249 329 282 120 76 aact:AA00312|3end_conserved_hypothetical_protein_(possible_hydroxypyruvate_isomerase)_212922..213072_POSITIVE 247 315 271 135 89 aact:AA02254|3end_2-oxoglutarate/malate_translocator_1536380..1536530_POSITIVE 247 39 4 88 51 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 241 314 277 142 95 aact:AA01279|3end_phage_exclusion-related_protein_868153..868303_POSITIVE 236 40 6 146 111 aact:AA02023|3end_NADH_dehydrogenase_1378115..1378265_POSITIVE 236 376 335 63 21 aact:AA00860|3end_phosphoheptose_isomerase_592527..592677_POSITIVE 233 233 199 148 115 aact:AA02946|3end_virulence_factor_protein_2060870..2061020_POSITIVE 232 402 364 138 102 aact:AA02077|3end_probable_outer_membrane_protein_1412708..1412858_POSITIVE 232 358 311 78 42 aact:AA01641|3end_hypothetical_protein_1111036..1111186_POSITIVE 232 235 192 63 7 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 231 338 300 126 80 aact:AA00370|3end_30S_ribosomal_protein_S10_249008..249158_POSITIVE 227 325 285 125 76 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 224 165 137 76 50 aact:AA02443|3end_cytochrome_C_related_protein_1686590..1686740_POSITIVE 224 165 137 121 95 aact:AA02444|3end_hypothetical_protein_1686635..1686785_POSITIVE 223 366 328 88 43 aact:AA02699|3end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1883159..1883309_POSITIVE 223 454 411 86 36 aact:AA00489|3end_hypothetical_protein_339032..339182_POSITIVE