Origin IGS: tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaggcagccatggtaaaagagtctgttttttactaggccttttactatggctgggatgtactttaaaatagtgatttatcaactagttggtgtctattgacgttaacttatgtttcttttcta Mask Tandem Repeat Region ================================================ tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Find is-nt database================================================ Query_seq: AA00436:AA00437|AA00436:AA00437:hypothetical protein:conserved hypothetical protein; possible parB-like nuclease:->->:302160..302283 124 tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00436:AA00437|AA00436:AA00437:hypothetical protein:conserved hypothetical protein; possible parB-like nuclease:->->:302160..302283 124 tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00436:AA00437|AA00436:AA00437:hypothetical protein:conserved hypothetical protein; possible parB-like nuclease:->->:302160..302283 124 tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Predict ORF larger than 30AA ================================================ tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 55 End: 84 ......................................................TTTTACTATGGCTGGGATGTACTTTAAAAT........................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00436:AA00437|AA00436:AA00437:hypothetical protein:conserved hypothetical protein; possible parB-like nuclease:->->:302160..302283 124 tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Intra-Species Hit: Count: 1 Min: 1 Max: 124 Len: 124 Subject: aact_AA00436_AA00437|hypothetical protein:conserved hypothetical protein; possible parB-like nuclease|POSITIVE:POSITIVE|[302160,302283]|124 HSP 1 e-value: 1.0E-64 bit: 246.0 Len: 124 Query Start:1 Query End:124 Subject Strand: POSITIVE Subject Start: 1 Subject End: 124 tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta tagaaaagaaacataagttaacgtcaatagacaccaactagttgataaatcactattttaaagtacatcccagccatagtaaaaggcctagtaaaaaacagactcttttaccatggctgcctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAAAGAAACAUAAGUUAACGUCAAUAGACACCAACUAGUUGAUAAAUCACUAUUUUAAAGUACAUCCCAGCCAUAGUAAAAGGCCUAGUAAAAAACAGACUCUUUUACCAUGGCUGCCUUA (((((.((..............(((....)))..(((....)))........)).)))))..........(((((((.((((((((.((.((.....)))).).))))))).)))))))..... (-23.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGCAGCCAUGGUAAAAGAGUCUGUUUUUUACUAGGCCUUUUACUAUGGCUGGGAUGUACUUUAAAAUAGUGAUUUAUCAACUAGUUGGUGUCUAUUGACGUUAACUUAUGUUUCUUUUCUA .....(((((((((((((((.(((((........)))))))))))))))))))).((((((((.......))))...))))....((((..((((....))))..))))............... (-39.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 255 120 73 192 142 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 250 120 73 174 124 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 232 120 71 134 85 aact:AA00895|5end_hypothetical_protein_621285..621515_POSITIVE 230 120 75 194 144 aact:AA01294|5end_DNA_adenine_methylase_876415..876645_POSITIVE 227 117 71 133 82 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 226 87 48 231 192 aact:AA01686|5end_hypothetical_protein_1137913..1138143_POSITIVE 224 69 23 99 42 aact:AA01140|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_781610..781840_POSITIVE 224 50 14 208 170 aact:AA00559|5end_conserved_hypothetical_protein_383167..383397_POSITIVE 220 119 72 207 161 aact:AA02052|5end_probable_exodeoxyribonuclease_VII_small_subunit_1398461..1398691_POSITIVE 218 117 73 43 5 aact:AA02325|5end_membrane_protein;_possible_ABC_transporter,_permease_1587309..1587539_POSITIVE 215 103 61 184 140 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 212 120 71 149 113 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 211 55 23 45 15 aact:AA02495|5end_conserved_hypothetical_protein_1731562..1731792_POSITIVE 209 117 73 204 165 aact:AA00086|5end_2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy_late_synthase_63437..63667_POSITIVE 208 120 71 44 7 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 206 120 72 178 134 aact:AA00974|5end_glucosamine-fructose-6-phosphate_679215..679445_POSITIVE 202 119 82 59 14 aact:AA00565|5end_30S_ribosomal_protein_S7_389972..390202_POSITIVE 201 52 17 188 155 aact:AA01143|5end_hypothetical_protein_783111..783341_POSITIVE 199 69 22 180 134 aact:AA01933|5end_hypothetical_protein_1306060..1306290_POSITIVE 196 119 85 54 17 aact:AA00615|5end_cation_transport_protein_420529..420759_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 120 71 116 66 aact:AA01858|3end_conserved_hypothetical_protein_1251537..1251687_POSITIVE 250 119 74 143 97 aact:AA00512|3end_conserved_hypothetical_protein_354649..354799_POSITIVE 244 100 53 145 103 aact:AA01608|3end_conserved_hypothetical_protein_1087977..1088127_POSITIVE 237 80 34 119 74 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 232 120 71 119 70 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 230 120 75 82 32 aact:AA01292|3end_single-stranded_DNA_binding_protein_876383..876533_POSITIVE 227 117 71 62 11 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 224 69 23 94 37 aact:AA01138|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_781685..781835_POSITIVE 218 117 73 46 8 aact:AA02324|3end_ABC_transporter_ATP_binding_protein_1587392..1587542_POSITIVE 212 120 71 118 82 aact:AA01546|3end_deoxyribose_operon_repressor_1036320..1036470_POSITIVE 206 120 72 120 76 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 205 118 72 129 81 aact:AA00976|3end_conserved_hypothetical_protein_682278..682428_POSITIVE 199 69 22 132 86 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 193 103 69 139 101 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 193 106 68 145 101 aact:AA01132|3end_possible_cell_filamentation_protein_775907..776057_POSITIVE 191 89 41 104 62 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 191 88 50 72 18 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 191 119 72 59 7 aact:AA00574|3end_regulatory_protein_392381..392531_POSITIVE 189 108 61 64 11 aact:AA02519|3end_DNA_gyrase_A_1752612..1752762_POSITIVE 186 120 72 125 71 aact:AA01654|3end_periplasmic_maltose-binding_protein_precursor_1121163..1121313_POSITIVE