Origin IGS: taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagaatacctttttagtttacaaatggctcaacgggcaccattgcaggagttcattgatgtggtttttaatacatacttttgtttaatccaaaaacatttacaatttggataaagcgcttgatttgactcaatttaaatatattatggtgagaaccatatactccatgctttgttttatcatcaggaataacatcaagaggacgatatcctattgttttaggaggaactatttgtacactccatatattgtagaaatctaccatctatgatccctacacagaatttgcagtaatctgcaaatcaacttcttagcttaacggttttgaagattttgtttatcctgaagataagtgagttgattaacttagtaggaaccttcgacaacctagttattaacttaattaataggaaataatatta Mask Tandem Repeat Region ================================================ taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Find is-nt database================================================ Query_seq: AA00435:AA00436|AA00435:AA00436:hypothetical protein:hypothetical protein:->->:301457..301877 421 taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00435:AA00436|AA00435:AA00436:hypothetical protein:hypothetical protein:->->:301457..301877 421 taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00435:AA00436|AA00435:AA00436:hypothetical protein:hypothetical protein:->->:301457..301877 421 taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 19 End: 117 .................. M K L I T R L S K V P T K L I N S L I F R I N K I F K T V K L R S ................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 158 End: 352 ............................................................................................................................................................. M V D F Y N I W S V Q I V P P K T I G Y R P L D V I P D D K T K H G V Y G S H H N I F K L S Q I K R F I Q I V N V F G L ..................................................................... Protein_Len: 34 Strand: + Start: 289 End: 390 ................................................................................................................................................................................................................................................................................................ M E S N Q A L Y P N C K C F W I K Q K Y V L K T T S M N S C N G A R ............................... Protein_Len: 57 Strand: - Start: 155 End: 325 .......................................................................................................................................................... L H Y I E V I Y P T Y L Y N R R F C Y S I T R K I N N R I I F C L M S Y I T R V M I Y K F Q T L D L A K D L N Y M ................................................................................................ Protein_Len: 30 Strand: - Start: 9 End: 98 ........ K R N I L N I V L N D F T G V L N I L E S I K L I F L I K M ................................................................................................................................................................................................................................................................................................................................... taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 77 HP_score: -8.5 Tail_Score: -3.59202 Start: 121 End: 138 Full_Region: AAGCTAAGAAGTTGA TTTGCAG ATTA CTGCAAA TTCTGTGTAGGGATC ........................................................................................................................TTTGCAGATTACTGCAAA........................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 58 HP_score: -4.0 Tail_Score: -4.07885 Start: 207 End: 227 Full_Region: catcaggaataacat caagagga cgata tcctattg ttttaggaggaacta ..............................................................................................................................................................................................................caagaggacgatatcctattg.................................................................................................................................................................................................. Find igs database================================================ Query_seq: AA00435:AA00436|AA00435:AA00436:hypothetical protein:hypothetical protein:->->:301457..301877 421 taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Intra-Species Hit: Count: 1 Min: 1 Max: 421 Len: 421 Subject: aact_AA00435_AA00436|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[301457,301877]|421 HSP 1 e-value: 0.0 bit: 835.0 Len: 421 Query Start:1 Query End:421 Subject Strand: POSITIVE Subject Start: 1 Subject End: 421 taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc taatattatttcctattaattaagttaataactaggttgtcgaaggttcctactaagttaatcaactcacttatcttcaggataaacaaaatcttcaaaaccgttaagctaagaagttgatttgcagattactgcaaattctgtgtagggatcatagatggtagatttctacaatatatggagtgtacaaatagttcctcctaaaacaataggatatcgtcctcttgatgttattcctgatgataaaacaaagcatggagtatatggttctcaccataatatatttaaattgagtcaaatcaagcgctttatccaaattgtaaatgtttttggattaaacaaaagtatgtattaaaaaccacatcaatgaactcctgcaatggtgcccgttgagccatttgtaaactaaaaaggtattctc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUUAUUUCCUAUUAAUUAAGUUAAUAACUAGGUUGUCGAAGGUUCCUACUAAGUUAAUCAACUCACUUAUCUUCAGGAUAAACAAAAUCUUCAAAACCGUUAAGCUAAGAAGUUGAUUUGCAGAUUACUGCAAAUUCUGUGUAGGGAUCAUAGAUGGUAGAUUUCUACAAUAUAUGGAGUGUACAAAUAGUUCCUCCUAAAACAAUAGGAUAUCGUCCUCUUGAUGUUAUUCCUGAUGAUAAAACAAAGCAUGGAGUAUAUGGUUCUCACCAUAAUAUAUUUAAAUUGAGUCAAAUCAAGCGCUUUAUCCAAAUUGUAAAUGUUUUUGGAUUAAACAAAAGUAUGUAUUAAAAACCACAUCAAUGAACUCCUGCAAUGGUGCCCGUUGAGCCAUUUGUAAACUAAAAAGGUAUUCUC .((((((......(((((((...)))))))....((((..(((((((((..((..((((....))))((((((((.((((((..((((....((((................))))..(((((((((....)))))))))...(((((..(((..........)))..)))))......((((.(.((.....)).))))).....(((.(((((...))))).))).))))..)))))).)))).......(((.(((((.((((((....))))))((((((((...((((......))))..(.((.(((((((...........))))))).)).)..))))))))....................))))))))...))))...)).))))).))))..)))).....))))))... (-86.09) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAAUACCUUUUUAGUUUACAAAUGGCUCAACGGGCACCAUUGCAGGAGUUCAUUGAUGUGGUUUUUAAUACAUACUUUUGUUUAAUCCAAAAACAUUUACAAUUUGGAUAAAGCGCUUGAUUUGACUCAAUUUAAAUAUAUUAUGGUGAGAACCAUAUACUCCAUGCUUUGUUUUAUCAUCAGGAAUAACAUCAAGAGGACGAUAUCCUAUUGUUUUAGGAGGAACUAUUUGUACACUCCAUAUAUUGUAGAAAUCUACCAUCUAUGAUCCCUACACAGAAUUUGCAGUAAUCUGCAAAUCAACUUCUUAGCUUAACGGUUUUGAAGAUUUUGUUUAUCCUGAAGAUAAGUGAGUUGAUUAACUUAGUAGGAACCUUCGACAACCUAGUUAUUAACUUAAUUAAUAGGAAAUAAUAUUA .......(((..((((((........(((.....)))..((..((((((((......((((((((((((...((((.....((((.(((((((...........))))))).)))).....((((((......))))))....)))).))))))))))))))))).)))..))..(((((.((((((..((((.(((.((((.....)))).))).....(((((.((.....)).).))))......(((((..(((..........)))..)))))..((.(((((((....)))))))))..((((..((((....))))..))))....))))..)))))).)))))..((((((((.((((..((.(..........).))..)))))))))))))))))).)))........... (-96.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 349 397 354 48 1 aact:AA01637|5end_ribonucleoside_diphosphate_reductase_1106657..1106887_POSITIVE 300 415 376 85 40 aact:AA01195|5end_acyl_carrier_protein_819849..820079_POSITIVE 287 398 358 82 42 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 281 149 101 119 62 aact:AA01728|5end_conserved_hypothetical_protein(related_to_HipA_protein)_1168337..1168567_POSITIVE 280 389 358 214 186 aact:AA00682|5end_S-adenosylmethionine-dependent_methyltransferase_466322..466552_POSITIVE 277 149 101 171 118 aact:AA01316|5end_hypothetical_protein_891262..891492_POSITIVE 275 404 361 149 115 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 271 149 101 219 171 aact:AA00064|5end_ribosomal_small_subunit_pseudouridine_synthase_48087..48317_POSITIVE 270 420 377 45 3 aact:AA00166|5end_conserved_hypothetical_protein_(possible_acetyltransferase)_117555..117785_POSITIVE 267 396 359 46 3 aact:AA00202|5end_conserved_hypothetical_protein_(possible_membrane_protein)_140546..140776_POSITIVE 267 405 369 195 165 aact:AA00002|5end_DNA_polymerase_III,_beta_subunit_1233..1463_POSITIVE 265 169 123 224 175 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 264 395 372 178 154 aact:AA00695|5end_uridine_kinase_475033..475263_POSITIVE 260 401 362 176 130 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 259 402 361 74 38 aact:AA02153|5end_iron(III)_ABC_transporter,_permease_protein_1472530..1472760_POSITIVE 259 395 359 40 4 aact:AA01165|5end_conserved_hypothetical_protein_798133..798363_POSITIVE 258 248 201 152 102 aact:AA01596|5end_conserved_hypothetical_protein;_possible_membrane_protein_1076200..1076430_POSITIVE 255 137 93 188 151 aact:AA02999|5end_conserved_hypothetical_protein_2094275..2094505_POSITIVE 251 221 181 158 112 aact:AA02595|5end_cytochrome_c_peroxidase_1805741..1805971_POSITIVE 251 404 364 228 187 aact:AA02050|5end_1-deoxy-D-xylulose_5-phosphate_synthase_1395625..1395855_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 312 395 355 149 107 aact:AA02541|3end_conserved_hypothetical_protein_1772644..1772794_POSITIVE 300 415 376 79 34 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 291 396 359 146 111 aact:AA02982|3end_pyruvate_formate-lyase_activating_enzyme_2085127..2085277_POSITIVE 284 394 358 145 100 aact:AA00486|3end_nucleoside_transporter_338026..338176_POSITIVE 281 149 101 122 65 aact:AA01727|3end_conserved_hypothetical_protein_(possible_HipA_protein)_1168420..1168570_POSITIVE 273 400 356 146 98 aact:AA01477|3end_type_I_restriction_enzyme_R_protein_994479..994629_POSITIVE 271 149 101 82 34 aact:AA00062|3end_bicyclomycin_resistance_protein_(Sulfonamide_resistance_protein)_48030..48180_POSITIVE 270 420 377 57 15 aact:AA00165|3end_conserved_hypothetical_protein_117647..117797_POSITIVE 259 402 361 74 38 aact:AA02152|3end_ferric_enterobactin_transport;_ABC_transporter_ATP-binding_protein_1472610..1472760_POSITIVE 258 248 201 120 70 aact:AA01595|3end_conserved_hypothetical_protein_1076248..1076398_POSITIVE 254 396 359 141 102 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 251 404 364 102 61 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 251 395 365 128 89 aact:AA00647|3end_protoporphyrinogen_oxidase_443314..443464_POSITIVE 250 237 192 145 94 aact:AA02257|3end_conserved_hypothetical_protein_1536849..1536999_POSITIVE 250 403 368 74 35 aact:AA01644|3end_ferric_transport_system_permease_protein_1114160..1114310_POSITIVE 249 395 361 71 30 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 248 399 372 35 1 aact:AA02038|3end_50S_ribosomal_protein_L35_1387326..1387476_POSITIVE 248 420 374 89 42 aact:AA01128|3end_hypothetical_protein_774202..774352_POSITIVE 246 398 363 120 81 aact:AA01726|3end_conserved_hypothetical_protein_(possible_HipA-like_protein_1168304..1168454_POSITIVE 246 259 212 78 39 aact:AA00527|3end_ATP-dependent_DNA_helicase_361463..361613_POSITIVE