Origin IGS: taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatccacttccttttcagccacttaaataggtaagtagattataaatgatttgggttataaggcgacaattatttgtatcgtttgacagttatttgtaatttta Mask Tandem Repeat Region ================================================ taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Find is-nt database================================================ Query_seq: AA00426:AA00427|AA00426:AA00427:conserved hypothetical protein:hypothetical protein:->->:288266..288369 104 taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00426:AA00427|AA00426:AA00427:conserved hypothetical protein:hypothetical protein:->->:288266..288369 104 taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00426:AA00427|AA00426:AA00427:conserved hypothetical protein:hypothetical protein:->->:288266..288369 104 taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Predict ORF larger than 30AA ================================================ taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00426:AA00427|AA00426:AA00427:conserved hypothetical protein:hypothetical protein:->->:288266..288369 104 taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Intra-Species Hit: Count: 1 Min: 1 Max: 104 Len: 104 Subject: aact_AA00426_AA00427|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[288266,288369]|104 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 104 Query Start:1 Query End:104 Subject Strand: POSITIVE Subject Start: 1 Subject End: 104 taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt taaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUACAAAUAACUGUCAAACGAUACAAAUAAUUGUCGCCUUAUAACCCAAAUCAUUUAUAAUCUACUUACCUAUUUAAGUGGCUGAAAAGGAAGUGGAUU .......................(((((........)))))......................(((((((((.(((.((((......)))).)))))))))))) (-15.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCCACUUCCUUUUCAGCCACUUAAAUAGGUAAGUAGAUUAUAAAUGAUUUGGGUUAUAAGGCGACAAUUAUUUGUAUCGUUUGACAGUUAUUUGUAAUUUUA .....(((((((................))).)))).(((((((((((((....((((...((((((((....)))..))))))))).)))))))))))))... (-17.09) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 244 57 16 180 139 aact:AA02779|5end_conserved_hypothetical_protein_1942742..1942972_POSITIVE 231 90 41 122 70 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 224 100 57 52 1 aact:AA01538|5end_conserved_hypothetical_protein_1031527..1031757_POSITIVE 220 53 16 207 167 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 214 60 16 217 171 aact:AA00616|5end_phosphatidate_cytidylyltransferase_421965..422195_POSITIVE 209 55 34 189 163 aact:AA02418|5end_peptidyl-tRNA_hydrolase_1661090..1661320_POSITIVE 197 100 71 173 145 aact:AA01794|5end_glycine_cleavage_system_transcription_activator_1209708..1209938_POSITIVE 194 51 22 89 62 aact:AA00549|5end_ferredoxin-type_protein_375563..375793_POSITIVE 192 52 23 60 18 aact:AA01891|5end_pyridoxine_kinase_1275922..1276152_POSITIVE 190 90 41 152 99 aact:AA00245|5end_hypothetical_protein_172834..173064_POSITIVE 189 75 37 200 166 aact:AA01295|5end_3-dehydroquinate_synthase_877272..877502_POSITIVE 187 100 74 127 99 aact:AA00794|5end_phosphohydrolase_543653..543883_POSITIVE 183 99 51 128 82 aact:AA00110|5end_ATPase_involved_in_DNA_replication_79085..79315_POSITIVE 182 100 84 19 3 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 181 43 7 189 145 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 179 75 34 96 56 aact:AA01645|5end_ferric_transport_ABC-related_ATP-binding_protein_1114016..1114246_POSITIVE 179 78 40 168 134 aact:AA00702|5end_conserved_hypothetical_protein_481660..481890_POSITIVE 178 100 74 221 189 aact:AA02241|5end_molybdenum_cofactor_biosynthesis_protein_C_1523464..1523694_POSITIVE 178 57 34 24 4 aact:AA02158|5end_TonB-dependent_outer_membrane_receptor_1476155..1476385_POSITIVE 177 53 35 224 206 aact:AA00422|5end_LapB_membrane_protein_285528..285758_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 57 17 94 59 aact:AA02157|3end_TonB-dependent_outer_membrane_receptor_1476305..1476455_POSITIVE 231 90 41 149 97 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 224 75 36 129 88 aact:AA02254|3end_2-oxoglutarate/malate_translocator_1536380..1536530_POSITIVE 224 100 57 52 1 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 220 53 16 116 76 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 194 51 22 48 21 aact:AA00547|3end_diheme_cytochrome_c_precursor_(periplasmic_nitrate_reductase)_375602..375752_POSITIVE 193 92 64 47 13 aact:AA00809|3end_possible_oxidoreductase_555435..555585_POSITIVE 192 100 64 41 1 aact:AA00615|3end_cation_transport_protein_422042..422192_POSITIVE 187 100 74 56 28 aact:AA00792|3end_conserved_hypothetical_protein_543662..543812_POSITIVE 183 99 51 116 70 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 182 100 52 93 44 aact:AA01984|3end_tellurite_resistance_protein;_hemagglutinin_homolog_1345754..1345904_POSITIVE 181 43 7 129 85 aact:AA01640|3end_restriction_enzyme_type_I_helicase_subunit_1110839..1110989_POSITIVE 181 100 51 133 92 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 181 100 51 133 92 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 179 95 51 47 10 aact:AA01162|3end_conserved_hypothetical_protein_(possible_transmembrane_protein)_795776..795926_POSITIVE 178 44 16 145 119 aact:AA02778|3end_hypothetical_protein_1942802..1942952_POSITIVE 177 53 35 122 104 aact:AA00420|3end_tRNA/rRNA_methyltransferase_285506..285656_POSITIVE 175 89 64 137 113 aact:AA01877|3end_conserved_hypothetical_protein_1264890..1265040_POSITIVE 175 52 17 133 99 aact:AA01255|3end_hypothetical_protein_852873..853023_POSITIVE 175 60 22 125 78 aact:AA01168|3end_6-phosphogluconolactonase_802018..802168_POSITIVE