Origin IGS: gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggttttctcgtgttcaacaatgataatttggcggctagaatactttatcgacacgaaaaaaacgataaaaaaatgcaaaaaagaagggattatgcgaaaatacgccaattttttattctgtaaaaactaaac Mask Tandem Repeat Region ================================================ gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Find is-nt database================================================ Query_seq: AA00422:AA00423|AA00422:AA00423:LapB membrane protein:GTP-binding protein:->->:286541..286672 132 gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00422:AA00423|AA00422:AA00423:LapB membrane protein:GTP-binding protein:->->:286541..286672 132 gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00422:AA00423|AA00422:AA00423:LapB membrane protein:GTP-binding protein:->->:286541..286672 132 gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 25 End: 132 ........................ M A Y F R I I P S F L H F F I V F F V S I K Y S S R Q I I I V E H E K T Protein_Len: 37 Strand: - Start: 13 End: 123 ............ L I F F Q R I K A Y D R R K Q M K K D N K E H R Y L I R A A L N D N N F M ......... Protein_Len: 37 Strand: - Start: 6 End: 113 ..... * N K C F L F N A Y K R M I G E K K C K K I T K K T D I F Y E L R W I I M ................... gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00422:AA00423|AA00422:AA00423:LapB membrane protein:GTP-binding protein:->->:286541..286672 132 gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttcttttttgcatttttttatcgtttttttcgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc Intra-Species Hit: Count: 1 Min: 1 Max: 132 Len: 132 Subject: aact_AA00422_AA00423|LapB membrane protein:GTP-binding protein|POSITIVE:POSITIVE|[286541,286672]|132 HSP 1 e-value: 2.0E-23 bit: 109.0 Len: 55 Query Start:78 Query End:132 Subject Strand: POSITIVE Subject Start: 78 Subject End: 132 .............................................................................cgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc .............................................................................cgtgtcgataaagtattctagccgccaaattatcattgttgaacacgagaaaacc HSP 2 e-value: 2.0E-20 bit: 99.6 Len: 50 Query Start:1 Query End:50 Subject Strand: POSITIVE Subject Start: 1 Subject End: 50 gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttc.................................................................................. gtttagtttttacagaataaaaaattggcgtattttcgcataatcccttc.................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUAGUUUUUACAGAAUAAAAAAUUGGCGUAUUUUCGCAUAAUCCCUUCUUUUUUGCAUUUUUUUAUCGUUUUUUUCGUGUCGAUAAAGUAUUCUAGCCGCCAAAUUAUCAUUGUUGAACACGAGAAAACC .....((((((.(...........((((((.......(((...............)))....((((((((............)))))))).........))))))....(((....)))....).)))))). (-19.46) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUUCUCGUGUUCAACAAUGAUAAUUUGGCGGCUAGAAUACUUUAUCGACACGAAAAAAACGAUAAAAAAAUGCAAAAAAGAAGGGAUUAUGCGAAAAUACGCCAAUUUUUUAUUCUGUAAAAACUAAAC ((((((.((((........))))....((((((..........(((((((............)))))))....((((...............))))......))))))..............)))))).... (-17.86) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 254 46 12 82 49 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 252 70 27 180 135 aact:AA00680|5end_sodium/proline_symporter_(proline_permease)_464899..465129_POSITIVE 245 62 32 230 199 aact:AA01746|5end_thiol:disulfide_interchange_protein_1180636..1180866_POSITIVE 245 102 67 214 173 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 245 51 25 119 99 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 243 125 83 205 157 aact:AA01634|5end_hypothetical_protein_1104324..1104554_POSITIVE 240 58 21 162 124 aact:AA00166|5end_conserved_hypothetical_protein_(possible_acetyltransferase)_117555..117785_POSITIVE 238 102 68 167 120 aact:AA01070|5end_DNA_repair_protein_740920..741150_POSITIVE 235 118 76 86 42 aact:AA02273|5end_cold_shock-like_protein_1549313..1549543_POSITIVE 234 119 81 223 173 aact:AA02131|5end_conserved_hypothetical_protein_(possible_TrpH_protein)_1454239..1454469_POSITIVE 234 89 45 76 31 aact:AA00072|5end_conserved_hypothetical_protein_54427..54657_POSITIVE 234 118 74 49 12 aact:AA00059|5end_heme_exporter_protein_B_(cytochrome_c-type_biogenesis_protein)_45412..45642_POSITIVE 233 117 76 74 34 aact:AA02228|5end_regulatory_protein,_sorC_family_1514981..1515211_POSITIVE 232 84 43 84 37 aact:AA00813|5end_hypothetical_protein_558807..559037_POSITIVE 231 59 19 139 96 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 227 46 15 76 38 aact:AA02879|5end_octaprenyl-diphosphate_synthase_2019523..2019753_POSITIVE 227 104 69 90 61 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 227 90 45 168 112 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 227 118 73 132 74 aact:AA00909.1|5end_hypothetical_protein_625970..626200_POSITIVE 226 105 69 209 171 aact:AA02177|5end_conserved_hypothetical_protein_1488963..1489193_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 266 117 73 60 18 aact:AA02043|3end_tagatose_6-phosphate_kinase_1391587..1391737_POSITIVE 260 104 63 118 75 aact:AA00663|3end_cystathionine_beta-lyase_455934..456084_POSITIVE 254 46 12 72 39 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 251 70 21 105 55 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE 245 48 12 47 13 aact:AA02944|3end_30S_ribosomal_protein_S20_2059028..2059178_POSITIVE 245 46 6 125 81 aact:AA02794|3end_myo-inositol_catabolism_protein_1962949..1963099_POSITIVE 245 62 32 146 115 aact:AA01745|3end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180632..1180782_POSITIVE 245 51 25 146 126 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 243 125 83 134 86 aact:AA01633|3end_probable_ferredoxin-like_protein_1104333..1104483_POSITIVE 240 70 26 133 92 aact:AA01818|3end_high-affinity_zinc_uptake_system_membrane_protein_1225078..1225228_POSITIVE 231 59 19 139 96 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 227 46 15 71 33 aact:AA02878|3end_conserved_hypothetical_protein_2019598..2019748_POSITIVE 227 104 69 109 80 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 227 90 45 121 65 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 226 105 69 132 94 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 226 119 76 71 35 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE 225 100 56 141 92 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 222 129 83 125 83 aact:AA00179|3end_conserved_hypothetical_protein_(possible_outer_membrane_protein)_127725..127875_POSITIVE 221 101 68 147 112 aact:AA02561|3end_conserved_hypothetical_protein_1784136..1784286_POSITIVE 220 103 73 144 112 aact:AA02243|3end_conserved_hypothetical_protein_1526411..1526561_POSITIVE