Origin IGS: taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggcatttgagttaaaaaacatcgtaaaaaaacaccgcacttttaaagacatttaagcccatcacaggatgggctttttgctatcagtttaaatgtgtta Mask Tandem Repeat Region ================================================ taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Find is-nt database================================================ Query_seq: AA00420:AA00422|AA00420:AA00422:tRNA/rRNA methyltransferase:LapB membrane protein:->->:285567..285667 101 taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00420:AA00422|AA00420:AA00422:tRNA/rRNA methyltransferase:LapB membrane protein:->->:285567..285667 101 taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00420:AA00422|AA00420:AA00422:tRNA/rRNA methyltransferase:LapB membrane protein:->->:285567..285667 101 taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Predict ORF larger than 30AA ================================================ taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -11.3 Tail_Score: -4.82154 Start: 27 End: 44 Full_Region: tttaaagacatttaa gcccatc acag gatgggc tttttgctatcagtt ..........................gcccatcacaggatgggc......................................................... Find igs database================================================ Query_seq: AA00420:AA00422|AA00420:AA00422:tRNA/rRNA methyltransferase:LapB membrane protein:->->:285567..285667 101 taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA00420_AA00422|tRNA/rRNA methyltransferase:LapB membrane protein|POSITIVE:POSITIVE|[285567,285667]|101 HSP 1 e-value: 2.0E-38 bit: 159.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgnnnnnnnacgatgttttttaactcaaatgccta taacacatttaaactgatagcaaaaagcccatcctgtgatgggcttaaatgtctttaaaagtgcggtgtttttttacgatgttttttaactcaaatgccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACAUUUAAACUGAUAGCAAAAAGCCCAUCCUGUGAUGGGCUUAAAUGUCUUUAAAAGUGCGGUGUUUUUUUACGAUGUUUUUUAACUCAAAUGCCUA ...(((.((((((..((((.....(((((((((....)))))))))...)))))))))).))).(((((((......((.(((....)))))))))))).. (-24.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGCAUUUGAGUUAAAAAACAUCGUAAAAAAACACCGCACUUUUAAAGACAUUUAAGCCCAUCACAGGAUGGGCUUUUUGCUAUCAGUUUAAAUGUGUUA ...(((((((((............((......)).............((.((...(((((((((....)))))))))..)))).....))))))))).... (-22.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 385 90 41 186 138 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 383 86 52 183 149 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 383 86 52 41 7 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 377 99 57 226 183 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 377 99 57 55 12 aact:AA02773|5end_hypothetical_protein_1936699..1936929_POSITIVE 367 98 57 144 100 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 351 67 27 88 45 aact:AA00618|5end_molybdopterin_biosynthesis_protein_422984..423214_POSITIVE 338 99 57 147 102 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 337 87 41 162 111 aact:AA01220|5end_competence_protein_F;_amidophosphoribosyltransferase_832775..833005_POSITIVE 334 80 35 131 85 aact:AA02465|5end_glucosamine-6-phosphate_isomerase_1704005..1704235_POSITIVE 328 89 41 230 191 aact:AA02193|5end_oxaloacetate_decarboxylase_gamma_chain_1494219..1494449_POSITIVE 326 99 51 144 89 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 322 69 27 185 134 aact:AA00864|5end_conserved_hypothetical_protein_598605..598835_POSITIVE 318 87 41 205 164 aact:AA02147|5end_hypothetical_protein_1469537..1469767_POSITIVE 318 87 41 105 64 aact:AA02148|5end_hypothetical_protein_1469437..1469667_POSITIVE 316 88 41 132 87 aact:AA00260|5end_conserved_hypothetical_protein_183315..183545_POSITIVE 313 81 44 134 90 aact:AA02249|5end_thiamine_biosynthesis_protein_1528365..1528595_POSITIVE 309 75 32 112 56 aact:AA02574|5end_dGTP_triphosphohydrolase_1792395..1792625_POSITIVE 306 90 50 184 140 aact:AA01020|5end_enonolase;_2-phosphoglycerate_dehydratase_712741..712971_POSITIVE 305 88 56 124 92 aact:AA02231|5end_probable_dethiobiotin_synthetase_1_1517650..1517880_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 385 90 41 103 55 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 383 86 52 89 55 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 377 99 57 52 9 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 347 67 25 95 45 aact:AA00420|3end_tRNA/rRNA_methyltransferase_285506..285656_POSITIVE 334 80 35 140 94 aact:AA02464|3end_hypothetical_protein_1704094..1704244_POSITIVE 318 87 41 65 24 aact:AA02146|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469477..1469627_POSITIVE 316 88 41 69 24 aact:AA00259|3end_conserved_hypothetical_protein_183332..183482_POSITIVE 309 75 32 66 10 aact:AA02573|3end_ABC_transporter,_ATP-binding_protein_1792429..1792579_POSITIVE 307 81 48 44 3 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 306 90 50 82 38 aact:AA01019|3end_conserved_hypothetical_protein_712719..712869_POSITIVE 300 78 32 140 106 aact:AA00003|3end_DNA_replication_and_repair_protein_3490..3640_POSITIVE 296 86 43 116 74 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 295 44 14 46 9 aact:AA02324|3end_ABC_transporter_ATP_binding_protein_1587392..1587542_POSITIVE 293 99 57 103 57 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 292 68 26 77 28 aact:AA02619|3end_GTP_cyclohydrolase_II_1826193..1826343_POSITIVE 290 67 30 60 23 aact:AA00865|3end_conserved_hypothetical_protein_600014..600164_POSITIVE 289 67 22 146 98 aact:AA02049|3end_hypothetical_protein_1395695..1395845_POSITIVE 287 98 57 104 58 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 287 66 27 53 30 aact:AA00417|3end_outer_membrane_lipoprotein_precursor_(probable_D-methionine-binding_lipoprotein)_284308..284458_POSITIVE 286 80 31 131 85 aact:AA02080|3end_ABC_transporter,_ATP-binding/permease_1414794..1414944_POSITIVE