Origin IGS: taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatgttctcggtggaataaaaataaacggctaattataagcataaaccttgccggctaaaagcgctcataaaaaatcctcagcacaacgcgaggatttgatggctgattttgggcggatta Mask Tandem Repeat Region ================================================ taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Find is-nt database================================================ Query_seq: AA00419:AA00420|AA00419:AA00420:conserved hypothetical protein (possible phosphatidylethanolamine-binding protein):tRNA/rRNA methyltransferase:->->:284972..285092 121 taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00419:AA00420|AA00419:AA00420:conserved hypothetical protein (possible phosphatidylethanolamine-binding protein):tRNA/rRNA methyltransferase:->->:284972..285092 121 taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00419:AA00420|AA00419:AA00420:conserved hypothetical protein (possible phosphatidylethanolamine-binding protein):tRNA/rRNA methyltransferase:->->:284972..285092 121 taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Predict ORF larger than 30AA ================================================ taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00419:AA00420|AA00419:AA00420:conserved hypothetical protein (possible phosphatidylethanolamine-binding protein):tRNA/rRNA methyltransferase:->->:284972..285092 121 taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Intra-Species Hit: Count: 1 Min: 1 Max: 121 Len: 121 Subject: aact_AA00419_AA00420|conserved hypothetical protein (possible phosphatidylethanolamine-binding protein):tRNA/rRNA methyltransferase|POSITIVE:POSITIVE|[284972,285092]|121 HSP 1 e-value: 8.0E-63 bit: 240.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc taatccgcccaaaatcagccatcaaatcctcgcgttgtgctgaggattttttatgagcgcttttagccggcaaggtttatgcttataattagccgtttatttttattccaccgagaacatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCGCCCAAAAUCAGCCAUCAAAUCCUCGCGUUGUGCUGAGGAUUUUUUAUGAGCGCUUUUAGCCGGCAAGGUUUAUGCUUAUAAUUAGCCGUUUAUUUUUAUUCCACCGAGAACAUC ..........(((((.(((....((((((((((.....)).)))))))).(((((((((.....((((.....))))..)))))))))......))).))))).................. (-23.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGUUCUCGGUGGAAUAAAAAUAAACGGCUAAUUAUAAGCAUAAACCUUGCCGGCUAAAAGCGCUCAUAAAAAAUCCUCAGCACAACGCGAGGAUUUGAUGGCUGAUUUUGGGCGGAUUA ..((((((....))))))..........(((.......)))..........(((.((((((..((.(((...((((((((.((.....)))))))))).)))))...)))))).))).... (-32.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 361 72 31 162 107 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 354 77 32 206 149 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 343 68 27 230 191 aact:AA02146|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1468828..1469058_POSITIVE 337 75 31 89 40 aact:AA00688|5end_curved_DNA-binding_protein_469165..469395_POSITIVE 335 70 28 75 24 aact:AA00711|5end_conserved_hypothetical_protein_487893..488123_POSITIVE 323 74 32 44 7 aact:AA02307|5end_5'-guanylate_kinase_1577271..1577501_POSITIVE 312 71 39 154 123 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 311 40 4 139 102 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 309 98 51 77 22 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 305 75 31 62 17 aact:AA01106|5end_conserved_hypothetical_protein_761675..761905_POSITIVE 301 42 5 177 131 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 301 75 33 50 10 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 300 45 7 198 157 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 299 67 23 167 126 aact:AA02827|5end_conserved_hypothetical_protein_1987021..1987251_POSITIVE 298 45 3 44 5 aact:AA01402|5end_beta_1-4_glucosyltransferase_947544..947774_POSITIVE 298 49 5 201 158 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 297 42 5 226 188 aact:AA01107|5end_adenylosuccinate_lyase_762310..762540_POSITIVE 296 76 37 41 3 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 296 68 24 179 132 aact:AA00275|5end_conserved_hypothetical_protein_192636..192866_POSITIVE 294 70 27 212 168 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 75 31 63 14 aact:AA00686|3end_hypothetical_protein_469219..469369_POSITIVE 312 71 39 148 117 aact:AA00990|3end_conserved_hypothetical_protein_693265..693415_POSITIVE 304 50 6 143 96 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 296 76 37 70 32 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 296 98 61 66 14 aact:AA01149|3end_glycyl-tRNA_synthetase_beta_subunit_788185..788335_POSITIVE 294 70 27 139 95 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 290 39 3 149 103 aact:AA02076|3end_conserved_hypothetical_protein_1411094..1411244_POSITIVE 287 45 3 139 95 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 286 68 22 56 6 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 284 76 32 143 83 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 280 74 31 150 93 aact:AA02747|3end_ribose-phosphate_pyrophosphokinase;_phosphoribosylpyrophosphate_synthase_1925258..1925408_POSITIVE 280 47 2 68 23 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 280 100 56 91 52 aact:AA01235.1|3end_50S_ribosomal_protein_L30_842535..842685_POSITIVE 279 76 31 68 29 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 278 47 6 46 9 aact:AA01808|3end_electron_transport_complex_protein_1219316..1219466_POSITIVE 278 60 19 87 51 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 278 80 31 129 84 aact:AA00448|3end_C-terminal_region_of_competence_protein_M_313058..313208_POSITIVE 276 80 32 81 31 aact:AA00647|3end_protoporphyrinogen_oxidase_443314..443464_POSITIVE 276 86 44 143 101 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 275 98 55 115 60 aact:AA02703|3end_hypothetical_protein_1885582..1885732_POSITIVE