Origin IGS: ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataagccctttttggtgtttaattttttatcgaaaggagctttgtattctagacgtctagaatgctatgtcaacatttatcagctatattcttataattatttgtttccattttaggacaaaatggcagaaggtttgcacgacataaaaataaagcagataatcagcaaaataatttatggacaggatgaataaaa Mask Tandem Repeat Region ================================================ ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Find is-nt database================================================ Query_seq: AA00414:AA00415|AA00414:AA00415:possible histidinol-phosphatase:D-methionine ABC transporter, ATP-binding protein:->->:281594..281789 196 ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00414:AA00415|AA00414:AA00415:possible histidinol-phosphatase:D-methionine ABC transporter, ATP-binding protein:->->:281594..281789 196 ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00414:AA00415|AA00414:AA00415:possible histidinol-phosphatase:D-methionine ABC transporter, ATP-binding protein:->->:281594..281789 196 ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 9 End: 110 ........ M L S I N Y F A D Y L L Y F Y V V Q T F C H F V L K W K Q I I I R I ...................................................................................... Protein_Len: 34 Strand: - Start: 84 End: 185 ................................................................................... F P F L Y N Y S Y L Q Y I N V Y C E L R R S Y L A G K S L F N F V M ........... ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 99 End: 128 ..................................................................................................GTCAACATTTATCAGCTATATTCTTATAAT.................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ PromScan Matrix: RpoN Strand: - Score: 85 Start: 58 End: 74 .........................................................ATGGCAGAAGGTTTGCA.......................................................................................................................... ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00414:AA00415|AA00414:AA00415:possible histidinol-phosphatase:D-methionine ABC transporter, ATP-binding protein:->->:281594..281789 196 ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Intra-Species Hit: Count: 1 Min: 1 Max: 196 Len: 196 Subject: aact_AA00414_AA00415|possible histidinol-phosphatase:D-methionine ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[281594,281789]|196 HSP 1 e-value: 1.0E-107 bit: 389.0 Len: 196 Query Start:1 Query End:196 Subject Strand: POSITIVE Subject Start: 1 Subject End: 196 ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat ttttattcatcctgtccataaattattttgctgattatctgctttatttttatgtcgtgcaaaccttctgccattttgtcctaaaatggaaacaaataattataagaatatagctgataaatgttgacatagcattctagacgtctagaatacaaagctcctttcgataaaaaattaaacaccaaaaagggcttat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUUCAUCCUGUCCAUAAAUUAUUUUGCUGAUUAUCUGCUUUAUUUUUAUGUCGUGCAAACCUUCUGCCAUUUUGUCCUAAAAUGGAAACAAAUAAUUAUAAGAAUAUAGCUGAUAAAUGUUGACAUAGCAUUCUAGACGUCUAGAAUACAAAGCUCCUUUCGAUAAAAAAUUAAACACCAAAAAGGGCUUAU .............((((.................(((((.(((.((((((((((..(.(((.......))))..(((((((......)))..))))....)))))))))).))).))))).((((((...((((((((((....))))))).....)))....))))))...................)))).... (-36.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAGCCCUUUUUGGUGUUUAAUUUUUUAUCGAAAGGAGCUUUGUAUUCUAGACGUCUAGAAUGCUAUGUCAACAUUUAUCAGCUAUAUUCUUAUAAUUAUUUGUUUCCAUUUUAGGACAAAAUGGCAGAAGGUUUGCACGACAUAAAAAUAAAGCAGAUAAUCAGCAAAAUAAUUUAUGGACAGGAUGAAUAAAA ..((((.((((((((((..........)))))))))).)))).(((((((((....))))))))).........(((((((..(((((.......((((((((....(((((((.....)))))))..((..((((((................))))))..))....)))))))))))))....))))))).... (-43.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 90 53 152 117 aact:AA02723|5end_hypothetical_protein_1906263..1906493_POSITIVE 291 90 41 136 74 aact:AA00596|5end_uridylate_kinase_407496..407726_POSITIVE 268 80 39 196 162 aact:AA00451|5end_hypothetical_protein_315068..315298_POSITIVE 264 80 31 139 93 aact:AA01628|5end_thiamin-monophosphate_kinase_1100888..1101118_POSITIVE 259 89 41 56 12 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 255 80 31 171 115 aact:AA00450|5end_outer_membrane_antigenic_lipoprotein_b_precursor_313820..314050_POSITIVE 253 93 52 58 11 aact:AA02671|5end_gluconokinase_1858322..1858552_POSITIVE 248 63 25 229 187 aact:AA02952|5end_lipoprotein_signal_peptidase_2065116..2065346_POSITIVE 247 71 31 159 117 aact:AA01743|5end_lysyl-tRNA_synthetase_1177808..1178038_POSITIVE 245 73 45 73 42 aact:AA00541|5end_conserved_hypothetical_protein_370140..370370_POSITIVE 243 72 31 138 91 aact:AA02218|5end_deoxyribose-phosphate_aldolase_(fragment?)_1509758..1509988_POSITIVE 243 89 52 151 115 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 239 77 33 149 112 aact:AA00202|5end_conserved_hypothetical_protein_(possible_membrane_protein)_140546..140776_POSITIVE 237 75 31 221 180 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 237 161 123 45 4 aact:AA02561|5end_conserved_hypothetical_protein_1783553..1783783_POSITIVE 233 78 31 192 135 aact:AA02978|5end_sufI_protein_precursor_homolog_2081813..2082043_POSITIVE 233 167 121 71 12 aact:AA00816|5end_lacZ_expression_regulator_560900..561130_POSITIVE 232 74 32 91 48 aact:AA01067|5end_30S_ribosomal_protein_S2_738898..739128_POSITIVE 232 89 51 230 194 aact:AA00540|5end_hypothetical_protein_370050..370280_POSITIVE 231 80 34 229 182 aact:AA00813|5end_hypothetical_protein_558807..559037_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 89 43 141 87 aact:AA02913|3end_ribosomal_large_subunit_pseudouridine_synthase_2040527..2040677_POSITIVE 264 80 31 117 71 aact:AA01626|3end_N_utilization_substance_protein_B_1100946..1101096_POSITIVE 251 60 15 99 50 aact:AA01556|3end_ABC_transporter_ATP-binding_protein_1044759..1044909_POSITIVE 248 63 25 146 104 aact:AA02950|3end_isoleucyl-tRNA_synthetase_2065113..2065263_POSITIVE 245 73 45 114 83 aact:AA00540|3end_hypothetical_protein_370261..370411_POSITIVE 243 72 31 135 88 aact:AA02217|3end_deoxyribose-phosphate_aldolase_1509835..1509985_POSITIVE 243 89 52 148 112 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 239 77 33 137 100 aact:AA00201|3end_prolipoprotein_diacylglyceryl_transferase_140614..140764_POSITIVE 237 161 123 42 1 aact:AA02560|3end_integral_membrane_protein_1783630..1783780_POSITIVE 237 89 49 58 14 aact:AA01286|3end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_871243..871393_POSITIVE 232 89 51 147 111 aact:AA00539|3end_ADP-L-glycero-D-manno-heptose-6-epimerase_370047..370197_POSITIVE 230 58 11 144 96 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 230 58 12 113 70 aact:AA00580|3end_conserved_hypothetical_protein_(possible_membrane_protein)_397628..397778_POSITIVE 229 80 31 102 39 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 228 80 31 105 47 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 225 61 30 80 41 aact:AA00062|3end_bicyclomycin_resistance_protein_(Sulfonamide_resistance_protein)_48030..48180_POSITIVE 223 88 51 133 106 aact:AA00396|3end_conserved_hypothetical_protein_265952..266102_POSITIVE 222 81 43 131 77 aact:AA01615|3end_lipoprotein_releasing_system_transmembrane_1092447..1092597_POSITIVE 221 74 53 147 126 aact:AA02722|3end_cytidine_deaminase_1906352..1906502_POSITIVE 220 166 121 150 94 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE